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Bagchi S.N.,Rani Durgavati University | Sondhia S.,Directorate of Weed Science Research | Agrawal M.K.,Excellent Bio Research Solutions Pvt. Ltd | Banerjee S.,Rani Durgavati University
Journal of Applied Phycology | Year: 2015

Whole cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of Anabaena fertilissima CCC597 revealed the presence of a microginin (Mr = 565 Da), which was purified using ODS C18 solid-phase extraction followed by quaternary methyl ammonium (QMA)-ion exchange and reverse-phase liquid chromatography. Its partial structure was deduced upon LC-MS/MS as β-amino-α-hydroxy-decanoic acid-Ala-MeLeu-Tyr. Microginin-565 inhibited rabbit angiotensin-converting enzyme activity at an apparent IC50 = 9.8 μM. © 2015 Springer Science+Business Media Dordrecht Source

Chaturvedi P.,Rani Durgavati University | Kumar Agrawal M.,Excellent Bio Research Solutions Pvt. Ltd | Nath Bagchi S.,Rani Durgavati University
Ecotoxicology and Environmental Safety | Year: 2015

On the basis of relative abundance, frequency and biovolume, the important value index ranks were assigned to individual cyanobacteria in phytoplankton samples collected from fourteen water resources of Central India. The mcyABDE genes were detected in all the blooms with Microcystis (-aeruginosa, -viridis, -panniformis, -botrys) as being the major constituent morphospecies. On the other hand, blooms composed of primarily Oscillatoria (-limosa,-agardhii, -laetevirens) along with Anabaena, Nostoc, Phormidium and Spirulina as sub-dominant forms exhibited quite a patchy distribution of one or the other mcy genes. Fifty percent of Microcystis- but none of the Oscillatoria dominant blooms produced microcystins-RR and desmethyl-RR at 0.03-0.41mgg-1 bloom dry mass. Traces of dissolved microcystin was detected in lake water, which is well below the WHO guideline. Irrespective of cyanobacterial composition and microcystin production ability, during the study period 43-64% of the cyanobacterial bloom samples exhibited association of viable but nonculturable forms of Vibrio cholerae O1 and O139, as evident from amplification of the antigen genes. We believe that spread of endemic cholera is the major threat associated with harmful algal blooms. © 2015 Elsevier Inc. Source

Patel C.,Excellent Bio Research Solutions Pvt. Ltd | Agrawal M.,Excellent Bio Research Solutions Pvt. Ltd | Agrawal M.K.,Excellent Bio Research Solutions Pvt. Ltd
International Aquatic Research | Year: 2014

Protease inhibitors are produced by a large number of cyanobacterial genera and their motive and mode of production are largely unknown. The present study showcases a comparative study of three isolates: DL02, DL08 and DL018 of Oscillatoria species collected from Central India, which possessed the same degree of protease inhibitory activities during isolation. These strains were grown under the same nutritional and environmental conditions in the laboratory for many generations, cultured biomasses were extracted, and screened for their inhibitory activity against three major proteases, i.e., trypsin, chymotrypsin and papain using an endpoint assay with casein and kinetic assay using synthetic chromogenic substrates. When cultured under the same conditions, three Oscillatoria strains showed different degrees of protease inhibition against all tested proteases in both the assay systems. Oscillatoria sp. DL08 showed highest inhibition (least IC50 value) against all the enzymes in both endpoint as well as kinetic assays while Oscillatoria sp. DL18 almost lost its protease inhibitory function against tested proteases. Further, papain inhibitory function of Oscillatoria sp. is shown for the first time. The study suggests that production of protease inhibitor(s) may not be entirely dependent on abiotic factors such as nutrient availability, light and temperature, but rather a more complex process having several biotic and abiotic factors working in tandem. © 2014, The Author(s). Source

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