European Foundation of Environmental science

Athens, Greece

European Foundation of Environmental science

Athens, Greece
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Deogenov V.A.,European Foundation of Environmental science | Kakuris K.K.,European Foundation of Environmental science | Zorbas Y.G.,Hypokinetic Biochemistry Institute | Luzhkov A.G.,Hypokinetic Biochemistry Institute
Tokai Journal of Experimental and Clinical Medicine | Year: 2010

Objectives: Microelement supplementation during Hypokinesia (HK; diminished movement) affects differently microelement metabolism from that of normal muscular activity. In view of the effect of trace element supplementation and HK upon microelement metabolism we investigated the effect of vanadium (V) supplements on tissue V content and V loss during HK. Methods: Studies were performed on 240 male Wistar rats during a pre-experimental period of 9 days and an experimental period of 98 days. Rats were equally divided into four groups: unsupplemented control rats (UCR), unsupplemented experimental rats (UER), supplemented control rats (SCR) and supplemented experimental rats (SER). A daily supplementation of 0.8 μmol vanadium sulfate was given to the rats in the SCR and SER groups. Muscle V content, plasma V level and V loss was measured in the experimental and control groups of rats. Results: The gastrocnemius muscle and right femur bone V content decreased (p < 0.05), and plasma V level and urinary and fecal V loss increased (p < 0.05) in the SER and UER groups compared to their preexperimental values and their respective control groups (SCR) and UCR). However, the tissue V content decreased more (p < 0.05) and plasma V level and V loss increased more (p < 0.05) in the SER group than in the UER group. The tissue V content and plasma V level and V loss did not change in the control groups of rats compared to the pre-experimental values. Conclusions: It is concluded that during HK V supplementation decreases more tissue V content and increases more V loss and plasma V level in V deficient tissue indicating lower V utilization.


Zorbas Y.G.,Institute of Hypokinetic Biochemistry | Kakuris K.K.,Institute of Hypokinetic Biochemistry | Federenko Y.F.,Institute of Hypokinetic Biochemistry | Deogenov V.A.,European Foundation of Environmental science
Nutrition | Year: 2010

Objective: The incompleteness of electrolyte utilization during hypokinesia and electrolyte supplementation is the defining factor of electrolyte metabolic changes, yet the effect of electrolyte supplementation and HK upon electrolyte utilization is poorly understood. To determine the influence of magnesium (Mg2+) supplementation and hypokinesia (diminished movement) on magnesium utilization, we investigated the use of Mg2+ supplementation to establish its effect upon muscle Mg2+ content and Mg22+ losses. Methods: This study was conducted in 40 physically healthy male volunteers during a pre-experimental period of 30 d and an experimental period of 364 d. Subjects were equally divided into four groups: unsupplemented control subjects (UCSs), unsupplemented experimental subjects (UESs), supplemented control subjects (SCSs), and supplemented experimental subjects (SESs). A daily supplementation of 3.0 mmol of magnesium-chloride per kilogram of body weight was given to subjects in the SCS and SES groups. Results: Muscle Mg2+ content decreased (P < 0.05) and plasma Mg2+ concentration and Mg2+ loss in urine and feces increased (P < 0.05) in the SES and UES groups compared with their pre-experimental levels and values in their respective control groups (SCS and UCS). Muscle Mg2+ content decreased more (P < 0.05) and plasma Mg2+ concentration and Mg2+ loss in urine and feces increased more (P < 0.05) in the SES group than in the UES group. The muscle Mg2+ content and plasma Mg2+ level and Mg2+ losses did not change in the control groups. Conclusion: Daily Mg2+ supplementation during prolonged hypokinesia decreases more muscle Mg2+ content and Mg2+-deficient muscle increases more Mg2+ loss in healthy subjects indicating lower Mg2+ utilization with than without Mg2+ supplementation. © 2010 Elsevier Inc.


Deogenov V.A.,European Foundation of Environmental science | Luzhkov A.G.,Higher Institute of Biochemistry | Kakuris K.K.,Higher Institute of Biochemistry | Federenko Y.F.,European Foundation of Environmental science
Biological Trace Element Research | Year: 2010

The incompleteness of electrolyte deposition during hypokinesia (HK; diminished movement) is the defining factor of electrolyte metabolic changes, yet the effect of prolonged HK upon electrolyte deposition is poorly understood. The objective of this investigation was to determine the effect of muscle calcium (Ca++) changes upon Ca++ losses during prolonged HK. Studies were conducted on 20 physically healthy male volunteers during a pre-experimental period of 30 days and an experimental period of 364 days. Subjects were equally divided in two groups: control subjects (CS) and experimental subjects (ES). The CS group ran average distances of 9.2 ± 1.2 km day-l, and the ES group walked average distances of 2.3 ± 0.2 km day-l. Muscle Ca++ contents, plasma Ca++ concentrations, and Ca++ losses in urine and feces were measured in the experimental and control groups of subjects. The muscle Ca++ contents decreased (p < 0.05), and plasma Ca++ levels and Ca++ losses in the urine and feces increased (p < 0.05) in the ES group compared with their pre-experimental levels and the values in their respective CS group. Muscle Ca++ contents and plasma Ca ++ levels and urinary and fecal Ca++ losses did not change in the CS group compared to their pre-experimental levels. It is concluded that prolonged HK increase plasma Ca++ concentrations and Ca++ losses in Ca++ deficient muscle indicating decreased Ca++ deposition. © 2010 Springer Science+Business Media, LLC.


Zorbas Y.G.,Institute of Hypokinetic Biochemistry | Deogenov V.A.,European Foundation of Environmental science | Kakuris K.K.,European Foundation of Environmental science | Federenko Y.F.,Institute of Hypokinetic Biochemistry
Trace Elements and Electrolytes | Year: 2011

Objective: Hypokinesia (HK) intensifies electrolyte losses in electrolyte deficient tissue, yet the mechanisms of electrolyte losses in electrolyte deficient tissue are unknown. The aim of this study was to determine muscle sodium (Na+) content and its effect on Na+ losses and the ability of the body to deposit Na+ during HK. Methods: Studies were conducted on 20 physically healthy male volunteers during a 30 day pre-experimental period and a 364 day experimental period. Subjects were equally divided into two groups: control subjects (CS) and experimental subjects (ES). The CS group ran average distances of 9.5 ± 1.2 km/day and the ES group walked average distances of 2.5 ± 0.4 km/day. Results: The muscle Na + content decreased and plasma Na+ level and Na + losses in urine and feces increased (p < 0.05) in the ES group compared with their pre-experimental levels and the values in their respective CS group. Muscle Na+ level and plasma Na+ level and urine and fecal Na+ loss did not show any changes in the CS group compared with their pre-experimental values. Conclusion: The conclusion was that muscle Na+ content decreases and Na+ losses increase in Na + deficient muscle due to the less efficient Na+ deposition attributable to many hypokinetic factors. ©2011 Dustri-Verlag Dr. K. Feistle.


Federenko Y.F.,Institute of Hypokinetic Biochemistry | Deogenov V.A.,European Foundation of Environmental science | Kakuris K.K.,European Foundation of Environmental science | Yerullis K.B.,Institute of Hypokinetic Biochemistry
Biological Trace Element Research | Year: 2011

Hypokinesia (HK) induces electrolyte losses in electrolyte-deficient tissue, yet the mechanisms of electrolyte losses in electrolyte-deficient tissue remain unknown. Mechanisms of electrolyte deposition could be involved. To determine the effect of prolonged HK on potassium (K +) deposition were measured muscle K + content and K + losses. Studies were conducted on 20 physically healthy male volunteers during 30 days pre-experimental period and 364 days experimental period. Subjects were equally divided into two groups: control subjects (CS) and experimental subjects (ES). The CS group was run average distances of 9.8±1.7 km day -1 and the ES group was walked average distances of 2.7±0.6 km day -1. Muscle K + content decreased (p<0.05) and plasma K + concentration, and K + losses in urine and feces increased (p<0.05) in the ES group compared to their pre-experimental level and the values in their respective CS group. Muscle K + content, plasma K + level, and urine and fecal K + losses did not show any changes in the CS group compared to their pre-experimental values. The conclusion was that K + losses in K +-deficient muscle of healthy subjects could have been attributable to the less efficient K + deposition inherently to prolonged HK. © 2010 Springer Science+Business Media, LLC.


Zorbas Y.G.,Hypokinetic Biochemistry Laboratory | Zorbas Y.G.,European Foundation of Environmental science | Deogenov V.A.,European Foundation of Environmental science | Fedorov M.A.,European Foundation of Environmental science | Federenko Y.F.,Hypokinetic Biochemistry Laboratory
Trace Elements and Electrolytes | Year: 2012

Objective: During hypokinesia (HK) and electrolyte supplementation occurs electrolyte deficiency, yet the mechanisms of electrolyte deficiency during HK and electrolyte supplementation remain poorly understood. Changes in electrolyte deposition may be involved. To determine the effect of magnesium (Mg 2+) supplementation and HK on Mg 2+ absorption we measured tissue Mg 2+ content and Mg 2+ losses. Methods: Studies were conducted on male Wistar rats during a pre-experimental period of 9 days and an experimental period of 98 days. Animals were equally divided into 4 groups: unsupplemented control rats (UCR), unsupplemented experimental rats (UER), supplemented control rats (SCR) and supplemented experimental rats (SER). Results: Gastrocnemius muscle and right femur bone Mg 2+content decreased, and plasma Mg 2+ concentration and urine and fecal Mg 2+ losses increased (p < 0.05) in the UER and SER compared with their pre-experimental values and the values in their respective control groups (UCR and SCR). However, muscle and bone Mg 2+ content decreased more, while plasma Mg 2+ level and urine and fecal Mg 2+ losses increased more (p < 0.05) in the SER than in UER. Conclusion: The current study indicates that tissue Mg 2+ level decreases more with than without Mg 2+ supplementation showing that during HK Mg 2+ supplementation does not improve tissue Mg 2+. ©2012 Dustri-Verlag Dr. K. Feistle.


PubMed | European Foundation of Environmental science
Type: Journal Article | Journal: The Tokai journal of experimental and clinical medicine | Year: 2011

Microelement supplementation during Hypokinesia (HK; diminished movement) affects differently microelement metabolism from that of normal muscular activity. In view of the effect of trace element supplementation and HK upon microelement metabolism we investigated the effect of vanadium (V) supplements on tissue V content and V loss during HK.Studies were performed on 240 male Wistar rats during a pre-experimental period of 9 days and an experimental period of 98 days. Rats were equally divided into four groups: unsupplemented control rats (UCR), unsupplemented experimental rats (UER), supplemented control rats (SCR) and supplemented experimental rats (SER). A daily supplementation of 0.8 mol vanadium sulfate was given to the rats in the SCR and SER groups. Muscle V content, plasma V level and V loss was measured in the experimental and control groups of rats.The gastrocnemius muscle and right femur bone V content decreased (p < 0.05), and plasma V level and urinary and fecal V loss increased (p < 0.05) in the SER and UER groups compared to their pre-experimental values and their respective control groups (SCR) and UCR). However, the tissue V content decreased more (p < 0.05) and plasma V level and V loss increased more (p < 0.05) in the SER group than in the UER group. The tissue V content and plasma V level and V loss did not change in the control groups of rats compared to the pre-experimental values.It is concluded that during HK V supplementation decreases more tissue V content and increases more V loss and plasma V level in V deficient tissue indicating lower V utilization.

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