Ethnopharmacology Unit

Tamil Nadu, India

Ethnopharmacology Unit

Tamil Nadu, India
SEARCH FILTERS
Time filter
Source Type

Shajeela P.S.,St Johns College | Kalpanadevi V.,Ethnopharmacology Unit | Mohan V.R.,Ethnopharmacology Unit
Journal of Applied Pharmaceutical Science | Year: 2012

The present study was designed to investigate the possible antidiabetic, hypolipidaemic and antioxidant effects of ethanol extract of Nymphaea pubescens tuber. Diabetes was induced in Albino rats by administration of alloxan monohydrate (150 mg/kg, body weight i.p). The ethanol extract of Nymphaea pubescens tuber at a dose of 200mg/kg and 500mg/kg body weight were administered at single dose per day to diabetes induced rats for a period of 14 days. The effect of ethanol extract of Nymphaea pubescens tuber extract on blood glucose, plasma insulin, urea creatinine, glycosylated haemoglobin, serum lipid profile (total cholesterol (TC), triglyceride (TG), low density lipoprotein- cholesterol (LDL-C), very low density lipoprotein- cholesterol (VLDL-C), high density lipoprotein- cholesterol (HDLC) and phospholipids (PL)), serum protein, albumin, globulin, serum enzymes (Serum glutamate pyruvate transaminases (SGPT), serum glutamate oxaloacetate transaminases (SGOT) and alkaline phosphate (ALP)), lipoprotein peroxidation (LPO), blood reduced glutathione (GSH), oxidative glutathione (GSSG), GSH/GSSG ratio, erythrocytes glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione S-transferase (GST) were measured in the diabetic rats. The ethanol extracts of Nymphaea pubescens tuber elicited significant (p<0.05) reductions of blood glucose, lipid parameters except HDL-C, serum enzymes and significantly increased HDL-C and antioxidant. The extract also caused significant increase in plasma insulin (p<0.05) in the diabetic rats. In conclusion, ethanol extract of Nymphaea pubescens tuber offers promising antidiabetic and hypolipidaemic effects that may be mainly attributed to its potent antioxidant potential. Further studies will be needed in future in order to determine which one or more of its active constituents have the main antidiabetic and hypolidaemic effects.


Muthulakshmi A.,Vo Chidambaram College | Jothibai Margret R.,Pope s college | Mohan V.R.,Ethnopharmacology Unit
Journal of Applied Pharmaceutical Science | Year: 2012

Feronia elephantum belongs to the family Rutaceae is well known in Indian traditional system for its traditional uses. The present investigation was carried out to determine the possible bioactive components of leaves and bark of F. elephantum using GC-MS analysis. 18 components from leaves and 14 components from bark of the above said plant were identified. The prevailing compounds in the ethanol extract of leaves of F. elephantum were 7- Norbornadienyl t-butyl ether (17.26%), 2-isopropyl-5-methyl-1- heptanol (11.40%), 1- Octanol,2-butyl (8.47%), Phenol, 4-[2-(dimethylamino)-ethyl]- (4.56%), 2,3- Dimethylquinolin-4(1H)-one (3.58%), Ethyl iso-allocholate (1.63%). The ethanol extract of F. elephantum bark contained, 2-Propenenitrile, 3-(3.4-dimethoxyphenyl)-(60.72%) was found as major component followed by phenol, 4-(3-hydroxy-I-propenyl)-2-methoxy-(9.35%), 3-(2-NAcetyl- N-methylaminoethyl)indol (1.15%), cholesta-8,24-dine-3-ol, 4-methyl-(3a'- 4a')- (0.86%) as the major components.


Murugan M.,Ethnopharmacology Unit | Mohan V.R.,Ethnopharmacology Unit
Journal of Applied Pharmaceutical Science | Year: 2011

Phytochemicals from the leaves and stem bark of Bauhinia purpurea and Hiptage benghalensis were extracted using different solvents of various polarities such as petroleum ether, chloroform, acetone, methanol and water. The antibacterial activity was carried out against Staphylococcus aureus, Klebsiella pneumoniae, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi by disc diffusion method. Among the solvent extracts, methanol extract was most effective against the tested microorganisms. Phytochemical analysis revealed the presence of alkaloids, coumarin, flavonoids, phenols, tannins and terpenoids.


Maruthupandian A.,Ethnopharmacology unit | Mohan V.R.,Ethnopharmacology unit
International Journal of ChemTech Research | Year: 2011

Pterocarpus marsupium Roxb. is one of the medicinally important plants belonging to the family Fabaceae, commonly known as "vengai" traditionally wood are used in the treatment of diabetes. In the present study the ethanol extract of wood and bark of Pterocarpus marsupium has been subjected to GC-MS analysis. Eight phytochemical constituents have been identified in wood and bark respectively. The major chemical constituentsare 3-0-methyl-d-glucose, n-Hexadecanoic acid, 1,2-Benzene-dicarboxylic acid, diisooctyl ester, Tetradecanoic acid and 9,12 - Octadecadienoic acid (Z,Z) in wood. D-Friedoolean -14-en-3-one and lupeal were the major constituent bark.


Nishanthini A.,Ethnopharmacology Unit | Mohan V.R.,Ethnopharmacology Unit
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To evaluate the total phenolic, flavonoid contents and in vitro antioxidant activity of methanol extract of Xanthosoma sagittifolium corm. Methods: Total phenolic content was estimated using the Folin Ciocalteu method. The flavonoid content was determined using aluminium chloride. In vitro antioxidant activities were evaluated by studying DPPH radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity, ABTS radical cation scavenging activity and reducing power capacity were determined using standard procedure. Results: Xanthosoma sagittifolium corm exhibited 0.32g100g-1 total phenolic; 0.26g100g-1 flavonoid and better scavenging activity of DPPH (78.22±0.56%), hydroxyl radical (69.11±0.21%), superoxide radical (83.27±0.08%) and ABTS radical cations(76.11±0.07%). Conclusions: The present studies confirm the methanol extracts have potential in vitro antioxidant activity. © 2012 Asian Pacific Tropical Biomedical Magazine.


Murugan M.,Ethnopharmacology Unit | Mohan V.R.,Ethnopharmacology Unit
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To evaluate the total phenolic, flavonoid contents and in vitro antioxidant activity of methanol extract of Dioscorea esculenta (Lour). Burkill. Methods: Total phenolic content was estimated using the Folin Ciocalteu method. The flavonoid content was determined using aluminium chloride. In vitro antioxidant activities and reducing power capacity were determined using standard methods. Results: Total phenolic content in methanol extract of Dioscorea esculenta was found to be 0.79g/100g and flavonoids content was found to be 0.26 g/100g. The extract was screened for its potential antioxidant activities using tests such as DPPH radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity, ABTS radical cation scavenging activity and reducing power activity. Conclusions: The present studies confirm the methanol extracts have potential in vitro antioxidant activity. The phytochemical phenols and flavonoids could be the reason for its antioxidant activity. © 2012 Asian Pacific Tropical Biomedical Magazine.


Maruthupandian A.,Ethnopharmacology unit | Mohan V.R.,Ethnopharmacology unit
International Journal of Phytomedicine | Year: 2011

Wattakaka volubilis (L.f) Stapf known to the Palliyars as "Kurinjan" is an important medicinal plant. The Palliyar tribe, inhabitants of Sirumalai hills, Western Ghats, Tamil Nadu. India, use this plant as antidiabetic. The present investigation deals with GC-MS analysis of ethnol extract of the above said plant. Fifteen compounds were identified.


Tresina P.S.,Ethnopharmacology Unit | Mohan V.R.,Ethnopharmacology Unit
International Journal of Food Science and Technology | Year: 2011

Effect of gamma irradiation on Vigna unguiculata subsp. unguiculata seeds (maroon-coloured seed coat) at various doses (2, 5, 10, 15 and 25kGy) on the physicochemical properties, proximate composition, vitamins (niacin and ascorbic acid) and antinutritional factors were analysed. No significant changes were recorded in the physicochemical properties of irradiated seeds. Gamma irradiation resulted in a significant increase in crude protein, while the crude lipid, crude fibre and ash contents resulted in a dose-dependent decrease. Gamma-irradiated seeds presented a significant decrease in the ascorbic acid and niacin content. Irradiation processing significantly reduced the level of L-DOPA, phytic acid, hydrogen cyanide, trypsin inhibitor activity, oligosaccharides and lectins. The total free phenolics, tannins and in vitro protein digestibility on irradiation showed a significant dose-dependent increase. Gamma irradiation seems to be a good procedure to improve the quality of legume seeds from the nutritional point of view. © 2011 The Authors. International Journal of Food Science and Technology © 2011 Institute of Food Science and Technology.


Kalidass C.,Ethnopharmacology unit | Mohan V.R.,Ethnopharmacology unit
Tropical and Subtropical Agroecosystems | Year: 2011

Five accessions of the underutilized legume itching bean (Mucuna pruriens (L.) DC.) var. pruriens), collected from five different agroclimatic regions in Western Ghats, Tamil Nadu, India were analysed for their proximate and mineral composition, vitamins (niacin and ascorbic acid), fatty acid profiles, amino acid profiles of total seed proteins, in vitro protein digestibility (IVPD) and certain antinutritional factors, to determine their potential as an alternative source to alleviate protein-energy-malnutrition among the people of Tamil Nadu. The crude protein ranged from 27.5 - 31.2%, crude lipid 6.6 - 8.8%, total dietary fibre 6.5 - 9.7%, ash 4.3 - 6.0% and carbohydrates 46.1 - 52.5%. The energy level of the seed (1576 - 1620 kJ100g -1 DM) was comparable with commonly consumed Indian pulses. The investigated seeds were rich in minerals such as Na, K, Ca, Mg and P. The fatty acid profiles revealed that the seed lipids contained higher concentrations of linoleic acid and palmitic acid. The essential amino acid profile of total seed proteins compared favourably with FAO/WHO (1991) requirement pattern, except that there were deficiencies of sulphur containing amino acids in all the five accessions. The IVPD of the five accessions ranged from 70.1 - 76.5%. The range of antinutritional factors were as follows: total free phenolics, 3.4 - 4.8%, tannins, 0.14 - 0.23%, L-Dopa, 5.4 - 7.0%, phytic acid, 478 - 648 mg100g -1, hydrogen cyanide, 0.24 - 0.38 mg100g -1 and trypsin inhibitor activity, 40.4 - 48.2 TIU mg -1 protein. Verbascose was the principal oligosaccharides in all accessions. Lower levels of phytohaemagglutinating activity for human erythrocytes of 'O' blood group than for 'A' and 'B' blood groups were found. The antinutritional fatty acid, behenic acid (1.28 - 2.74%) was also detected.


Kalpanadevi V.,Ethnopharmacology Unit | Mohan V.R.,Ethnopharmacology Unit
LWT - Food Science and Technology | Year: 2013

The effects of hydration, cooking, autoclaving, germination and their combination on the reduction/elimination of antinutrients and the improvement of in vitro protein digestibility of the tribal pulse, Vigna unguiculata subsp. unguiculata were investigated. Hydration and germination processes were less effective in reducing l-Dopa and trypsin inhibitor activity. Cooking and autoclaving of presoaked seeds drastically reduced the content of total free phenolics, tannins, phytic acid, hydrogen cyanide, trypsin inhibitors and oligosaccharides. Germination (96 h) process reduced the total free phenolics, tannins, hydrogen cyanide and phytic acid. The combination of germination followed by autoclaving completely eliminated the total free phenolics, tannins, hydrogen cyanide, phytic acid, trypsin inhibitor, oligosaccharides and phytoheamagglutinating activity. The combination process (germination 96 h + autoclaving) improved the in vitro protein digestibility. We concluded that, a prolonged period of germination followed by autoclaving completely destroyed some of the antinutritional factors. © 2012 Elsevier Ltd.

Loading Ethnopharmacology Unit collaborators
Loading Ethnopharmacology Unit collaborators