Ethiraj College for Women Autonomous

Chennai, India

Ethiraj College for Women Autonomous

Chennai, India
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Kothai S.,Ethiraj College for Women Autonomous | Jayanthi B.,Amjain College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: Propolis is a mixture of plant resins and bee secretions. This study is to evaluate the antioxidant and antimicrobial activity of stingless bee propolis (Tetragonulairidipennis), rearedfromPudukottai region of Tamilnadu, India and also to determine the total phenol and total flavonoid contents of the sample responsible for these properties. Methods: Stingless bee propolis was extracted by ultra sonication method and was characterized by UV-Visible, FT-IR and SEM analysis. Total Phenolic and flavonoid contents were determined by using Folin-Ciocalteu spectrophotometric method and Aluminium chloride colorimetric method respectively. DPPH radical scavenging assay was used to find the antioxidant activity of the sample. Antibacterial activity was determined by using standard agar well diffusion method. Results: Total polyphenol content of the sample was 150μg/ml of Gallic acid equivalent (GAE) and the flavonoid content was 6mg/g of Quercetin equivalent (QE). The antioxidant potential of stingless bee propolis is found to be 83%. The sample showed significant antimicrobial activity against various human pathogens. Conclusion: Stingless bee propolis (Tetragonulairidipennis) collected from Pudukottairegion, Tamilnadu is a potential natural antioxidant source and is a promising antimicrobial drug for various bacterial infections. © 2014, IJPPS. All rights reserved.


Ranjitham A.M.,Ethiraj college for women Autonomous | Suja R.,Ethiraj college for women Autonomous | Caroling G.,Ethiraj college for women Autonomous | Tiwari S.,Ethiraj college for women Autonomous
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objective: Vegetable mediated synthesis of nanoparticles is a green chemistry approach that links Nanotechnology and Biotechnology. The present study is focussed on the biosynthesis of silver nanoparticles using aqueous fresh Cauliflower floret extract and to investigate the free radical scavenging potential, antimicrobial activity of the nanoparticles against different human pathogens and its cytotoxic activity against MCF-7 breast cancer cell line. Methods: It was found that aqueous silver ions when treated with aqueous extract of fresh Cauliflower floret are reduced in solution there by leading to the formation of silver nanoparticles under optimum conditions at pH 6. The formation of silver nanoparticles was indicated by the colour change from colourless to reddish brown. Biosynthesized nanoparticles was characterised using several techniques, viz- UV-Vis spectroscopy, FT-IR, XRD, TEM, SEM and EDAX analysis. The free radical scavenging potential was measured by DPPH assay, antimicrobial activity against four microorganisms was tested using disc diffusion method and cytotoxcity of the nanoparticles was determined against MCF-7 cell line at different concentrations by MTT assay. Results: Water soluble antioxidant constituents present in the Cauliflower floret extract were mainly responsible for the reduction of silver ions to nanosized Ag particles. UV-Vis spectral analysis showed silver Surface Plasmon Resonance band at 425nm. The presence of active proteins and phenolic groups present in the biomass before and after reduction was identified by FT-IR. The presence of elemental silver was characterised by EDS. The crystalline morphology and size of the nanoparticles were determined by TEM, SEM, and X-ray diffraction studies which showed the average size of the nanoparticles in the range 40 -50nm, as well as revealed their FCC structure. The biologically synthesized nanoparticles efficiently inhibited pathogenic organisms such as Klebsiella Pneumonia, Staphylococcus Saprophyticus and E. Coli. The biosynthesized nanoparticles might serve as a potent antioxidant as revealed by DPPH assay. Further these nanoparticles efficiently showed reduced viability and increased cytotoxicity on MCF-7 breast cancer cell line in a dose dependent manner. Conclusion: The present investigation revealed that the fresh aqueous Cauliflower floret extract are capable of producing silver nanoparticles extra cellularly through green synthesis and the reduction process was proved to be good, competent, convenient, easy to handle and considered ecofriendly as an alternative route to physical and chemical methods. The biosynthesized nanoparticles are quite stable in aqueous solution for a month without any sign of precipitation.


Mercy Ranjitham A.,Ethiraj College for Women Autonomous | Selva Ranjani G.,Ethiraj College for Women Autonomous | Caroling G.,Ethiraj College for Women Autonomous
International Journal of PharmTech Research | Year: 2015

The synthesis of metal nanoparticles is a growing area for research due to its potentiality in the application and development of advanced technologies. In general, nanoparticles are synthesized by using chemical methods which are not eco-friendly. In this study we used the fast, convenient, eco-friendly method for synthesis of copper nano particles by using thefresh aqueous pineapple extract. The fresh aqueous pineapple extract was mixed with copper sulphate solution by heating to a temperature of 60-80˚C and under optimum condition at ph 11. The reduction reaction was studied by observing the color change from blue to reddish brown. Water soluble antioxidant constituents present in the fresh aqueous pineapple extract were mainly responsible for the reduction of copper ions to nano sized Cu particles. The resulting copper nanoparticles were characterized by UV Visible Absorption Spectrometer, powder X-Ray Diffraction (PXRD), Fourier Transform Infrared (FTIR), Scanning Electron Microscopy (SEM) and EDAX analysis. UV-Vis spectral analysis showed Copper nanoparticles exhibit an absorption peak at around 576 nm. X-ray diffraction analysis shows that the crystalline morphology of nanoparticles are FCC in nature. The FTIR spectrum analysis has confirmed the presence of functional groups and active proteins in the biomass before and after reduction. SEM results display the formation of copper nanoparticles with an average size of 30-50 nm. The presence of elemental copper was characterized by EDS. The antibacterial and antifungal activity of these nanoparticles was studied against E-Coil, Staphylococcus aureus, Aspergillus niger and Trichoderma viride. The biosynthesized nanoparticles are quite stable in aqueous solution for a month without any sign of precipitation. © 2015, Sphinx Knowledge House. All rights reserved.


Chathurdevi G.,Ethiraj College for Women Autonomous | Uma Gowrie S.,Ethiraj College for Women Autonomous
International Journal of Current Pharmaceutical Review and Research | Year: 2016

Objective: Medicinal plants are gaining worldwide attention owing to the fact that the herbal drugs are cost effective, easily available and with negligible side effects. Medicinal plants harbour endophytic microflora and they are valuable source of Bioprospecting endophytes. Endophytes are the microorganisms that inhabit interior of plant tissues that shows no apparent harm to host. Endophytic fungi from medicinal plants are known as "Promising Source" of bioactive novel metabolites which has significant role in pharmacology, agriculture and in industries. Methods: In our present research work, endophytic fungi were isolated from the leaves of medicinal plant Cardiospermum halicacabum. The frequency of the presence of endophytic fungi was studied based on seasonal variation. Seven endophytic fungi were isolated, of which two isolates were sporulating and identified based on colony morphology, sporulating structures and taken for further studies. The ethyl acetate extract of the isolates were screened for phytochemical studies and also assayed for invitro antibacterial activity. Further, the fungal isolates were checked for their ability to produce extracellular enzymes by qualitative assay and were used to study the presence of chitosan on their cell wall. Results: The isolates were also screened for extracellular biosynthesis of Silver nanoparticles for which UV-VIS absorption was recorded. FT-IR analysis was also performed to confirm the presence of various functional groups. Conclusion: The endophytes from Cardiospermum halicacabum can be a potent natural source of bioactive compounds which can be considered to be prolific resource for drugs and as Bioprotectants. Further investigation will focus on the strain improvement and genomic variation among these isolates. © 2016 The Authors.


Caroling G.,Ethiraj College for Women Autonomous | Tiwari S.K.,Ethiraj College for Women Autonomous | Mercy Ranjitham A.,Ethiraj College for Women Autonomous | Suja R.,Ethiraj College for Women Autonomous
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

OBJECTIVE- The synthesis of metal nanoparticles is a growing area of research due to its potentiality in the application and development of advanced technologies. In general, nanoparticles are synthesized by using chemical methods which are not eco-friendly. Vegetable mediated synthesis of nanoparticles is a green chemistry approach that connects the nanotechnology and biotechnology. In the present investigation we report a green chemistry approach, for the biological synthesis of silver nanoparticles using Broccoli floret aqueous extract under optimum conditions METHOD- Here we have used a fast, convenient and environment friendly method for the synthesis of silver nanoparticles by biologically reducing AgNO3 with aqueous extract of Broccoli florets (Brassica Oleracea L. var. Italica) under optimum conditions (pH-6-7). The formation of silver nanoparticles was indicated by the colour change from colourless to reddish brown. Biosynthesized nanoparticles were characterized by UV-VIS, FT-IR, XRD, SEM, TEM and EDAX analysis. The free radical scavenging activity was assessed by DPPH assay. These biologically synthesised Ag nanoparticles were tested for antimicrobial activity against four human pathogens viz. Klebsiella Pneumonia, Staphylococcus Saprophyticus, Bacillus Cereus and Escheria Coli. These nanoparticles were assessed further for cytotoxic activity on MCF-7 cell line. RESULT- The reduction process was simple and convenient to handle and monitored by UV-Vis spectroscopy which showed surface plasmon resonance at 425nm. The presence of active proteins and phenolic groups present in biomass before and after reduction was identified by FT-IR. The crystalline morphology and size of the nanoparticles were determined by TEM, SEM AND X-ray diffraction studies, which showed the average particle size of silver nanoparticles was in the range 40- 50nm as well as revealed their FCC structure. Presence of elemental silver was revealed by EDAX analysis. These biologically synthesised Ag nanoparticles were found to be effective in controlling growth of human pathogens viz. Klebsiella Pneumonia, Staphylococcus Saprophyticus and Escheria Coli. These nanoparticles showed high % toxicity against MCF-7 cell line. The reducing property of aqueous extract is due to the presence of antioxidant viz. ascorbic acid, polyphenols which is confirmed by quantitative assay and scavenging effect of free radicals proved by DPPH scavenging activity. CONCLUSION- The present investigation revealed that the fresh Broccoli floret aqueous extract is capable of producing silver nanoparticles that are quiet stable for 15 days at room temperature without any sign of precipitation.


Vidyalakshmi A.,Ethiraj College for Women Autonomous | Kruthika K.,Ethiraj College for Women Autonomous
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To test efficacy of Parmelia perlata (P. perlata), which is used in traditional medicine for rapid wound healing against test bacteria that cause wound infections. Methods: Different solvents such as methanol, ethyl acetate and acetone were used for extraction of P. perlata. The sensitivity of the test bacteria to solvent extracts of P. perlata was tested by measuring the zone of inhibition on growth media and by determining the minimal inhibitory concentration and minimal bactericidal concentration. Results: Methanol, ethyl acetate and acetone extracts of P. perlata have shown inhibitory activity against Staphylococcus aureus (S. aureus). Conclusions: The results of the present study indicate that P. perlata has potential antibacterial compounds against S. aureus that causes multitude of skin infections among human beings. Development of drugs from natural compounds can help us to combat antibiotic-resistant bacteria. © 2012 Asian Pacific Tropical Biomedical Magazine.


Jayanthi C.,Ethiraj College for Women Autonomous | Revathi K.,Ethiraj College for Women Autonomous
Biochemical and Cellular Archives | Year: 2016

The scope of the present work is to analyze the effect of SAAf 75% (Carbendazim 12% + Mancozeb 63%) on carbohydrate and glucose metabolism in fresh water fish Labeorohita(rohu) exposed to SAAf 75% for a period of 21 days. Preliminary acute toxicity tests were carried out to find the median lethal tolerance limit (LC50) of the fish to the pesticide mixture for 96hrs and 95% confidence limits were calculated. The 96hr LC50value for 75% was found to be 16.62 mg/L. One fortieth (0.41 mg/L)and one-twentieth (0.83 mg/L) value was taken as the two sub-lethal concentrations for the study. The fishes were maintained in two different sub-lethal concentrations for a period of 21 days anda control group was also maintained for the same period. An overall significant reduction (P < 0.01) was witnessed in the total carbohydrate and glucose content of all the tissues exposed to both the sub-lethal concentration when compared to the control group of fishes, at regular intervals of 7, 14, and 21 days.


Kothai S.,Ethiraj College for Women Autonomous | Jayanthi B.,Amjain College
International Journal of ChemTech Research | Year: 2015

Stingless beekeeping is very rare in Tamilnadu. Propolis of Stingless bees is a mixture of plant resins and bee secretions – it possesses a wide spectrum of medicinal properties. The chemical composition and the bio medical applications of stingless bee propolis vary depending on the geographicallocation of the region, itsClimate, season and the availability of the botanical sources from which the bees forage.This present study compares the impact of environment on the Total phenolic,Flavonoid contents and anti-bacterial activity of two stingless bee propolis sample, one reared from Pudukottai region of Tamilnadu and other one reared from the most commercial hub of Chennai region, namely Thyagarayar Nagar. Total polyphenol content of the Chennai sample is 10 μg/ml of Gallic acid equivalent (GAE) and the flavonoid content is 48μg /ml of Quercetin equivalent (QE). This is very low when compared to the pudukottai sample, total poly phenol of which is 150μg/ml of Gallic acid equivalent (GAE) and the flavonoid content is 6mg/g of Quercetin equivalent (QE). This is reflected in their antibacterial activity against different human pathogens also.This study shows the impact of highly polluted environment on the quality of the Chennai propolis sample and reveals that the environment is unsuitable for the existence of Stingless bees. © 2015 Sphinx Knowledge House. All Rights Reserved.


Gangadharan R.,Ethiraj College for Women Autonomous | Sethusankar K.,Rkm Vivekananda College Autonomous | Murugan G.,University of Madras | Bakthadoss M.,University of Madras
Acta Crystallographica Section E: Structure Reports Online | Year: 2011

In the title compound, C22H18N2O 2, the pyran ring of the chromene unit is fused with an isoxazole ring, which adopts an N-envelope conformation with the N atom lying 1.3291 (14) Å from the mean plane of the remaining ring atoms [maximum deviation = 0.341 (2) Å]. The dihedral angle between the isoxazole and chromene units is 43.74 (8)° and that between the iosxazole ring and the naphthalene ring system is 58.82 (8)°. In the crystal, the molecules are linked by weak C - H⋯π interactions.


Kothai S.,Ethiraj College for Women Autonomous | Jayanthi B.,Amjain College
International Journal of ChemTech Research | Year: 2014

Ultrasonic waves appear as an interesting way to improve process productivity.The present study reports a facileultrasonicsintensified greensynthesis of silver nano particles from the extract of Camellia Sinensisfortified with lemon and honey. The extract is acting both as reducing agentas well as capping agent.The synthesized silver nano particles have been characterized by UV-Vis spectroscopy, Scanning Electron Microscopy(SEM) and Energy Dispersive X-ray (EDX) Analysis. The active organic groups responsible for the reduction of Ag+ ions to elemental silver were identified by Fourier Transform Infrared spectroscopy(FT-IR).The effect of ultrasound on the reduction reaction rate was found andwas found to get enhanced with ultrasonic irradiation.Polydispersity of synthesized silver nano particles is minimized and alsocontrolled by ultrasonication method. This novel method is rapid, facile and combines the advantage of both sonochemistry and green chemistry.

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