Entity

Time filter

Source Type

Somerville, NJ, United States

Toedter G.,Centocor | Li K.,Centocor | Marano C.,Centocor | Ma K.,Centocor | And 5 more authors.
American Journal of Gastroenterology | Year: 2011

Objectives: Infliximab has been shown to induce clinical response and remission in ulcerative colitis (UC). To characterize the biological response of patients to infliximab, we analyzed the mRNA expression patterns of mucosal colonic biopsies taken from UC patients enrolled in the Active Ulcerative Colitis Trial 1 (ACT1) study. Methods: Biopsies were obtained from 48 UC patients before treatment with 5 or 10 mg/kg infliximab, and at 8 and 30 weeks after treatment (n113 biopsies). Global gene expression profiling was performed using Affimetrix GeneChip Human Genome U133 Plus 2.0 arrays. Expression profiling results for selected genes were confirmed using qPCR. Results: Infliximab had a significant effect on mRNA expression in treatment responders, with both infliximab dose and duration of treatment having an effect. Genes affected are primarily involved with inflammatory response, cell-mediated immune responses, and cell-to-cell signaling. Unlike responders, non-responders do not effectively modulate T H1, T H2, and T H17 pathways. Gene expression can differentiate placebo and infliximab responders. Conclusions: Analysis of mRNA expression in mucosal biopsies following infliximab treatment provided insight into the response to therapy and molecular mechanisms of non-response. © 2011 by the American College of Gastroenterology. Source


Desai C.,New Jersey Institute of Technology | Meng X.,New Jersey Institute of Technology | Yang D.,Ethicon Research and Development | Wang X.,New Jersey Institute of Technology | And 2 more authors.
Journal of Crystal Growth | Year: 2011

Antisolvent synthesis of micron scale Griseofulvin was carried out with simultaneous suspension stabilization under low power ultrasonic agitation. The organic solvent plays an important role because the supersaturation could be varied by using different solvents and the physicochemical characteristics of the suspension are also altered, which affects stability. In this study we present the effect of solvents on particle formation, polymorphism and stability of micron scale Griseofulvin formation in aqueous media. © 2010 Elsevier B.V. All rights reserved. Source


Meng X.,New Jersey Institute of Technology | Yang D.,Ethicon Research and Development | Keyvan G.,Rutgers University | Michniak-Kohn B.,Rutgers University | Mitra S.,New Jersey Institute of Technology
Colloids and Surfaces B: Biointerfaces | Year: 2012

The anti-solvent synthesis in presence of cyclodextrins (CDs) of the drug Griseofulvin (GF) is presented. This was followed by immobilization into cellulosic polymer films suitable for drug delivery. The results show that 72% of the GF precipitated in presence of CD, while the rest led to the formation of a water soluble GF/CD complex. The cyclodextrins were effective in inhibiting particle growth and stabilizing the drug suspensions. Among the CDs tested here, hydroxypropyl-β-cyclodextrin (HPBCD) was found to be most effective in reducing the particle size. The release profiles from the cyclodextrin stabilized GF particles showed improvement in release rate, which indicated effective drug/cyclodextrin interactions. © 2011 Elsevier B.V. Source


Meng X.,New Jersey Institute of Technology | Yang D.,Ethicon Research and Development | Mitra S.,New Jersey Institute of Technology
Journal of Applied Polymer Science | Year: 2011

The antisolvent synthesis of micrometer-scale particles, their stabilization in suspension, and their subsequent self-assembly as homogeneous polymer films suitable for drug delivery were studied. Ultrasonic agitation was used in the precipitation of the drug particulates, stabilization was carried out with hydroxypropyl methylcellulose (HPMC), and finally, drug-encapsulated films containing HPMC and polyvinylpyrrolidone were synthesized. These contained as much 28% of the drug Griseofulvin, and the particles were distributed uniformly throughout the films. Most importantly, the redispersion of the drug-loaded films in an aqueous matrix showed that the crystallinity remained unaltered, and there was no appreciable increase in the particle size distribution. © 2010 Wiley Periodicals, Inc. Source


Meng X.,New Jersey Institute of Technology | Yang D.,Ethicon Research and Development | Keyvan G.,Rutgers University | Michniak-Kohn B.,Rutgers University | Mitra S.,New Jersey Institute of Technology
Colloids and Surfaces B: Biointerfaces | Year: 2011

The anti-solvent synthesis of micron-scale particles, their stabilization, and subsequent self-assembly into polymer films suitable for drug delivery is presented. The colloidal particles were stabilized using low molecular weight hydroxypropyl methylcellulose (HPMC), while drug encapsulation was carried out with high molecular weight HPMC and polyvinylpyrrolidone (PVP). Griseofulvin (GF) was used as the model drug compound, and the polymer films were evaluated in terms of their surface morphology, mechanical properties and in vitro drug release. In general, the release rates were best described by first-order and Hixson-Crowell kinetic models, and in a typical film containing 57% HPMC, 100% of GF was released within 50. min. © 2011 Elsevier B.V. Source

Discover hidden collaborations