Yruela I.,Estacion Experimental de Aula Dei Consejo Superior de Investigaciones Cientificas |
Contreras-Moreira B.,Estacion Experimental de Aula Dei Consejo Superior de Investigaciones Cientificas |
Magalha es C.,University of Minho |
Magalha es C.,ICVS 3Bs PT Government Associate Laboratory |
And 6 more authors.
Genome Biology and Evolution | Year: 2016
The advent of whole-genome sequencing has provided an unprecedented detail about the evolution and genetic significance of species-specific variations across the whole Mycobacterium tuberculosis Complex. However, little attention has been focused on understanding the functional roles of these variations in the protein coding sequences. In this work, we compare the coding sequences from 74 sequenced mycobacterial species including M. africanum, M. bovis, M. canettii, M. caprae, M. orygis, and M. tuberculosis. Results show that albeit protein variations affect all functional classes, those proteins involved in lipid and intermediary metabolism and respiration have accumulated mutations during evolution. To understand the impact of these mutations on protein functionality, we explored their implications on protein ductility/disorder, a yet unexplored feature of mycobacterial proteomes. In agreement with previous studies, we found that a Gly71Ile substitution in the PhoPR virulence system severely affects the ductility of its nearby region in M. africanum and animal-adapted species. In the same line of evidence, the SmtB transcriptional regulator shows amino acid variations specific to the Beijing lineage, which affects the flexibility of the N-terminal trans-activation domain. Furthermore, despite the fact that MTBC epitopes are evolutionary hyperconserved, we identify strain- and lineagespecific amino acid mutations affecting previously known T-cell epitopes such as EsxH and FbpA (Ag85A). Interestingly, in silico studies reveal that these variations result in differential interaction of epitopes with the main HLA haplogroups. © The Author 2016.
Fernandez V.,Technical University of Madrid |
Khayet M.,Complutense University of Madrid |
Montero-Prado P.,Aragon Institute of Engineering Research |
Heredia-Guerrero J.,University of Seville |
And 8 more authors.
Plant Physiology | Year: 2011
The surface of peach (Prunus persica 'Calrico') is covered by a dense indumentum, which may serve various protective purposes. With the aim of relating structure to function, the chemical composition, morphology, and hydrophobicity of the peach skin was assessed as a model for a pubescent plant surface. Distinct physicochemical features were observed for trichomes versus isolated cuticles. Peach cuticles were composed of 53% cutan, 27% waxes, 23% cutin, and 1% hydroxycinnamic acid derivatives (mainly ferulic and p-coumaric acids). Trichomes were covered by a thin cuticular layer containing 15% waxes and 19% cutin and were filled by polysaccharide material (63%) containing hydroxycinnamic acid derivatives and flavonoids. The surface free energy, polarity, and work of adhesion of intact and shaved peach surfaces were calculated from contact angle measurements of water, glycerol, and diiodomethane. The removal of the trichomes from the surface increased polarity from 3.8% (intact surface) to 23.6% and decreased the total surface free energy chiefly due to a decrease on its nonpolar component. The extraction of waxes and the removal of trichomes led to higher fruit dehydration rates. However, trichomes were found to have a higher water sorption capacity as compared with isolated cuticles. The results show that the peach surface is composed of two different materials that establish a polarity gradient: the trichome network, which has a higher surface free energy and a higher dispersive component, and the cuticle underneath, which has a lower surface free energy and higher surface polarity. The significance of the data concerning water-plant surface interactions is discussed within a physiological context. © 2011 American Society of Plant Biologists.
Panagos P.,European Commission - Joint Research Center Ispra |
Ballabio C.,European Commission - Joint Research Center Ispra |
Borrelli P.,European Commission - Joint Research Center Ispra |
Meusburger K.,University of Basel |
And 11 more authors.
Science of the Total Environment | Year: 2015
Rainfall is one the main drivers of soil erosion. The erosive force of rainfall is expressed as rainfall erosivity. Rainfall erosivity considers the rainfall amount and intensity, and is most commonly expressed as the R-factor in the USLE model and its revised version, RUSLE. At national and continental levels, the scarce availability of data obliges soil erosion modellers to estimate this factor based on rainfall data with only low temporal resolution (daily, monthly, annual averages). The purpose of this study is to assess rainfall erosivity in Europe in the form of the RUSLE R-factor, based on the best available datasets. Data have been collected from 1541 precipitation stations in all European Union (EU) Member States and Switzerland, with temporal resolutions of 5 to 60min. The R-factor values calculated from precipitation data of different temporal resolutions were normalised to R-factor values with temporal resolutions of 30min using linear regression functions. Precipitation time series ranged from a minimum of 5years to a maximum of 40years. The average time series per precipitation station is around 17.1years, the most datasets including the first decade of the 21st century. Gaussian Process Regression (GPR) has been used to interpolate the R-factor station values to a European rainfall erosivity map at 1km resolution. The covariates used for the R-factor interpolation were climatic data (total precipitation, seasonal precipitation, precipitation of driest/wettest months, average temperature), elevation and latitude/longitude. The mean R-factor for the EU plus Switzerland is 722MJmmha-1h-1yr-1, with the highest values (>1000MJmmha-1h-1yr-1) in the Mediterranean and alpine regions and the lowest (<500MJmmha-1h-1yr-1) in the Nordic countries. The erosivity density (erosivity normalised to annual precipitation amounts) was also the highest in Mediterranean regions which implies high risk for erosive events and floods. © 2015 The Authors.
Angulo-Martinez M.,Estacion Experimental de Aula Dei Consejo Superior de Investigaciones Cientificas |
Begueria S.,Estacion Experimental de Aula Dei Consejo Superior de Investigaciones Cientificas
Hydrology and Earth System Sciences | Year: 2012
Rainfall erosivity refers to the ability of precipitation to erode soil, and depends on characteristics such as its total volume, duration, and intensity and amount of energy released by raindrops. Despite the relevance of rainfall erosivity for soil degradation prevention, very few studies have addressed its spatial and temporal variability. In this study the time variation of rainfall erosivity in the Ebro Valley (NE Spain) is assessed for the period 1955-2006. The results show a general decrease in annual and seasonal rainfall erosivity, which is explained by a decrease of very intense rainfall events whilst the frequency of moderate and low events increased. This trend is related to prevailing positive conditions of the main atmospheric teleconnection indices affecting the West Mediterranean, i.e. the North Atlantic Oscillation (NAO), the Mediterranean Oscillation (MO) and the Western Mediterranean Oscillation (WeMO). © 2012 Author(s).