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Rio de Janeiro, Brazil

Estácio is a private educational company focused in the universities sector founded in 1970 in Rio de Janeiro, Brazil. It is named after the Portuguese knight and military officer Estácio de Sá, who was the founder of the city of Rio de Janeiro. It is Brazil's second largest university with over 311,900 students in 57 campuses around the country, 39 of which are located in the state of Rio de Janeiro. Estácio is the second largest higher education company in Brazil by number of students and revenues, after Kroton Educacional.The soccer club Universidade Estácio de Sá Futebol Clube is owned by the university. Wikipedia.

Teixeira L.A.,Estacio de Sa University
Historia, Ciencias, Saude - Manguinhos | Year: 2015

This paper discusses the knowledge and medical practices relating to cervical cancer in Brazil. It analyses the growing medical interest in the disease at the beginning of the twentieth century, the development of prevention techniques in the 1940s, and the emergence of screening programs in the 1960s. It argues that the development of knowledge on cervical cancer was related simultaneously to a number of factors: transformations in medical knowledge, the development of the idea that the disease should be treated as a public health problem, the increased concerns with women’s health, and major changes to the Brazilian healthcare system. The article concludes by identifying a number of issues that are still proving to be obstacles to control of the disease. © 2014, Fundacao Oswaldo Cruz, All right reserved. Source

Ricucci D.,Piazza Calvario | Siqueira Jr. J.F.,Estacio de Sa University
Journal of Endodontics | Year: 2013

Introduction: This article reports a case of persistent apical periodontitis lesion in a mesiobuccal root of a maxillary molar subjected to single-visit endodontic treatment. Methods: The treatment protocol followed endodontic standards including using nickel-titanium instruments with working length ending 0.5-mm short of the apex, establishment and maintenance of apical foramen patency, irrigation with 5% NaOCl, smear layer removal, a final rinse with and ultrasonic agitation of chlorhexidine, and filling by the vertical compaction technique. Even so, the lesion in the mesiobuccal root became larger in size after follow-up examination at 1 year 6 months, and periradicular surgery was performed. Radiographic control after 11 months showed that periradicular healing was almost complete. The root apex and the lesion were analyzed histologically and histobacteriologically. Results: The lesion was diagnosed as a "pocket cyst," and no bacteria were noted extraradicularly. The cause of continued disease was a heavy bacterial biofilm infection located in an intricate network of apical ramifications. Bacteria were also observed on the walls of one of the mesiobuccal canals packed between the obturation material and the root canal wall. Conclusions: This case report reinforces the need for treating the infected root canal as a complex system that possesses anatomic intricacies in which bacteria can spread and remain unaffected by treatment procedures. © 2013 American Association of Endodontists. Source

Vogel I.N.,Estacio de Sa University | Macedo A.F.,Rio de Janeiro State Federal University
Plant Cell, Tissue and Organ Culture | Year: 2011

We investigated the influence of light quality on in vitro germination and protocorm formation, and the effect of indole-3-acetic acid (IAA) and thidiazuron (TDZ) on proliferation of protocorm-like bodies (PLBs) and development of plantlets of Cyrtopodium glutiniferum Raddi. Germination was faster under white and blue light, and highest under green light. The protocorm developed more rapidly under white, blue, and green light. Continuous darkness delayed seed germination and reduced protocorm formation. Among the plant growth regulators (PGRs) tested for multiplying PLBs, shoots, and roots from protocorms, IAA proved to be superior. TDZ was effective in inducing PLB fresh weight accumulation, but not morphogenesis, unlike IAA. This study indicated that C. glutiniferum seedlings can be produced in vitro using asymbiotic seed germination techniques. High germination rate and protocorm yield can be obtained by initially cultivating C. glutiniferum seeds on medium without growth regulators under white light, or under white light supplemented with green or blue light. This culture system complies with commercial and conservation requirements for rapid and low-cost propagation. © 2010 Springer Science+Business Media B.V. Source

Siqueira Jr. J.F.,Estacio de Sa University
Methods in molecular biology (Clifton, N.J.) | Year: 2010

Application of nucleic acid technology to the analysis of the bacterial diversity in the oral cavity in conditions of health and disease has not only confirmed the findings from early culture studies but also significantly expanded the list of oral inhabitants and candidate pathogens associated with the major oral diseases. Over 800 bacterial distinct species-level taxa have been detected in the oral cavity and recent studies using high-throughput technology suggest that the breadth of bacterial diversity can be much larger. This chapter provides an overview of the diversity and taxonomy of oral bacteria. Emphasis is also given on nucleic acid technologies that have been widely used for the study of the oral microbiota. Source

Rocas I.N.,Estacio de Sa University | Siqueira J.F.,Estacio de Sa University
Anaerobe | Year: 2012

Fourty-one bacterial strains isolated from infected dental root canals and identified by 16S rRNA gene sequence were screened for the presence of 14 genes encoding resistance to beta-lactams, tetracycline and macrolides. Thirteen isolates (32%) were positive for at least one of the target antibiotic resistance genes. These strains carrying at least one antibiotic resistance gene belonged to 11 of the 26 (42%) infected root canals sampled. Two of these positive cases had two strains carrying resistance genes. Six out of 7 Fusobacterium strains harbored at least one of the target resistance genes. One Dialister invisus strain was positive for 3 resistance genes, and 4 other strains carried two of the target genes. Of the 6 antibiotic resistance genes detected in root canal strains, the most prevalent were blaTEM (17% of the strains), tetW (10%), and ermC (10%). Some as-yet-uncharacterized Fusobacterium and Prevotella isolates were positive for blaTEM, cfxA and tetM. Findings demonstrated that an unexpectedly large proportion of dental root canal isolates, including as-yet-uncharacterized strains previously regarded as uncultivated phylotypes, can carry antibiotic resistance genes. © 2012 Elsevier Ltd. Source

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