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Rusanov K.,Agro Bio Institute | Kovacheva N.,Essential Medical | Rusanova M.,Agro Bio Institute | Atanassov I.,Agro Bio Institute
Industrial Crops and Products | Year: 2012

The composition of volatile compounds in the rose essential oils obtained from the rose flowers has been known to be affected by a number of factors including the region of rose growing. The variations of the flower volatile composition of Rosa damascena plants growing in 11 different rose plantations along the famous Rose Valley in Bulgaria were studied following GC/MS metabolite profiling. ANOVA statistical test revealed that only 14 of a total of 90 identified compounds were significantly influenced by the geographic location of the sampled rose plantation. The hierarchical clustering analysis based on the subset of these 14 compounds revealed no clear correlation between the levels of rose flower volatiles and geographic location of the studied rose plantations. The results from the study suggest low variability of rose flower volatiles accumulation and elevated plasticity of the common R. damascena genotype industrially cultivated in the Rose Valley. © 2011 Elsevier B.V.

Rusanov K.,Agrobioinstitute Dragan Tsankov Blvd. | Kovacheva N.,Essential Medical | Rusanova M.,Agrobioinstitute Dragan Tsankov Blvd. | Atanassov I.,Agrobioinstitute Dragan Tsankov Blvd.
Food Chemistry | Year: 2011

Industrial cultivation of Rosa damascena and rose oil production is a centuries-old industry based on well-established traditional hand-picking of rose flowers. Here the traditional rose harvesting practices were evaluated through GC/MS analysis of the volatile compounds accumulation in R. damascena flowers at eight different developmental stages, flower buds with arrested development and flowers collected at six daytime points related to flower picking. The accumulation of 80 out of 100 volatile compounds was significantly influenced by the stage of flower development while 62 out of 103 compounds were significantly influenced by the daytime period of flower collection. Principal component analysis showed close clustering of the flower developmental stages and daytime periods preferable for flower harvesting and production of high quality and quantity of rose oil. Taken together the obtained results provide further support to the currently applied traditional rose flower harvesting practices. The possible applications of more precise flower harvesting for fine modulation of rose oil composition and reduction levels of the unwanted compound methyl eugenol are discussed. © 2011 Elsevier Ltd. All rights reserved.

Guyden J.C.,City College of New York | Martinez M.,Tuskegee University | Chilukuri R.V.E.,City College of New York | Reid V.,University of Iowa | And 2 more authors.
Current Molecular Medicine | Year: 2015

Thymic nurse cells (TNCs) are specialized epithelial cells that reside in the thymic cortex. The initial report of their discovery in 1980 showed TNCs to contain up to 200 thymocytes within specialized vacuoles in their cytoplasm. Much has been reported since that time to determine the function of this heterotypic internalization event that exists between TNCs and developing thymocytes. In this review, we discuss the literature reported that describes the internalization event and the role TNCs play during T cell development in the thymus as well as why these multicellular complexes may be important in inhibiting the development of autoimmune diseases. © 2015 Bentham Science Publishers.

Essential Medical | Date: 2014-03-06

A method of sealing a vascular puncture of a vessel can include the step of inserting a guidewire through a sheath and into the vessel. The guidewire can include a hollow tube and a balloon coupled to a distal end of the hollow tube. The method can further include the steps of inflating the balloon so as to temporarily occlude the vessel and guiding a closure device along the hollow tube toward the puncture such that a toggle of the closure device enters the vessel through the puncture; and sealing the puncture with the closure device.

Essential Medical | Date: 2012-09-06

A closure device for sealing a percutaneous puncture in a wall of a body passageway, the closure device including at least one of a toggle configured to engage an interior surface of the body passageway or a plug configured to engage an exterior surface of the body passageway and a guide wire configured to extend from an outside of the body to inside the body passageway, wherein at least one of the toggle and the plug is associated with the guide wire.

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