Fan Y.-J.,Beijing Jishuitan Hospital |
Liu B.,Capital Medical University |
Wang L.-D.,Henan Key Laboratory for Esophageal Cancer Research |
Li L.,Capital Medical University |
Lan Y.,Beijing Jishuitan Hospital
World Chinese Journal of Digestology | Year: 2011
AIM: To investigate the methylation status of the promoter region of the RASSF1A gene in gastric cardiac adenocarcinoma (GCA) and esophageal squamous cell carcinoma (ESCC) in the distal esophagus and to analyze their clinical significance. METHODS: Thirty-three GCA patients and 36 ESCC patients who came from a high-incidence region of ESCC in Linzhou, Henan and were treated at Yaocun Esophageal Cancer Hospital and Linzhou Center Hospital were enrolled in this study. No statistical differences were found in sex, age, and tumor differentiation between GCA and ESCC patients. No patients received chemotherapy or radiotherapy before operation. Methylation-specific polymerase chain reaction (MSP) was used to investigate the methylation status of the promoter region of the RASSF1A gene in the two groups of patients. RESULTS: For GCA patients, the frequencies of RASSF1A promoter methylation in cancer tissue (CA), matched dysplasia tissue (DYS) and normal tissue (NOR) were 63.6%, 20% and 4.2%, respectively. For ESCC patients, the frequencies of RASSF1A promoter methylation in tumor tissue, matched dysplasia tissue and normal tissue were 66.7%, 25% and 16.7%, respectively. High methylation frequency was found in both types of cancer tissue. With the evolution of lesions (NOR-DYS-CA), the frequency of RASSF1A promoter methylation showed an increasing tendency in both GCA (χ2 = 22.173, P < 0.001) and ESCC patients (χ2 = 19.324, P < 0.001). The frequency of RASSF1A promoter methylation in normal tissue from GCA patients was lower than that from ESCC patients. CONCLUSION: RASSF1A promoter hypermethylation is a molecular event that occurs in both GCA and ESCC patients. RASSF1A is a potential candidate biomarker for early detection of carcinoma of the esophagogastric junction.