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Ripoll G.,CSIC - Centro de Investigación y Tecnología Agroalimentaria | Alcalde M.J.,Escuela Tecnica Superior de Ingenieria Agronomica | Horcada A.,Escuela Tecnica Superior de Ingenieria Agronomica | Campo M.M.,University of Zaragoza | And 3 more authors.
Meat Science | Year: 2012

The effects of breed and slaughter weight on chemical composition, fatty acid groups, texture, and sensory characteristics of meat of 141 suckling male kids from 5 Spanish breeds were studied. There was a decrease in texture and lightness and hue angle with the increase of the slaughter weight. Fatty acid composition was correlated with the intramuscular fat content. All the breeds except MO had values of n-6/n-3 ratio below 4, which is the healthy limit recommended, and a low atherogenic index as well as a low intramuscular fat content. A multivariate analysis discriminated light kid, which had the most tender and juicy meat, from heavy kid which had more intense kid and milk odours. Blanca Andaluza and Pirenaica had most tender and juicy meat. The effect of slaughter weight on meat traits should be considered separately for each breed to find the most appropriate meat according to consumers preferences. © 2012 Elsevier Ltd.

Antolinos V.,Escuela Tecnica Superior de Ingenieria Agronomica | Antolinos V.,Technical University of Cartagena | Fernandez P.S.,Escuela Tecnica Superior de Ingenieria Agronomica | Fernandez P.S.,Technical University of Cartagena | And 5 more authors.
Foodborne Pathogens and Disease | Year: 2012

Strains belonging to Bacillus cereus Group include six different species, among which are Bacillus thuringiensis, Bacillus weihenstephanensis, and Bacillus cereus sensu stricto, a causative agent of food poisoning. Sequence of the panC-housekeeping gene is used for B. cereus Group affiliation to seven major phylogenetic groups (I-VII) with different ecological niches and variations in thermal growth range and spore heat resistance of B. cereus Group microorganisms varies among phylogenetic groups. We assigned a selection of B. cereus sensu stricto strains related to food poisoning from the Spanish cultivar Collection (Valencia) to Group IV strains based on panC gene sequence. Thermal inactivation assays revealed variability of spore heat resistance within these Group IV strains. Adequate food sanitizing treatments therefore require fast and reliable identification of particular strains. In the present study, feasibility of genotyping via high-resolution melting (HRM) analysis was examined. HRM analysis of amplified polymorphic 16S-23 intergenic spacer region (ISR) region proved to be discriminatory for B. cereus sensu stricto strain typing, while two other polymorphic regions within the bacterial rRNA operon allowed differentiation between Bacillus species, demonstrating its applicability for discrimination on the species and strain level within B. cereus Group. © Copyright 2012, Mary Ann Liebert, Inc. 2012.

Fernandez J.E.,Institute Recursos Naturales y Agrobiologia IRNAS CSIC | Moreno F.,Institute Recursos Naturales y Agrobiologia IRNAS CSIC | Martin-Palomo M.J.,Escuela Tecnica Superior de Ingenieria Agronomica | Cuevas M.V.,Institute Recursos Naturales y Agrobiologia IRNAS CSIC | And 2 more authors.
Environmental and Experimental Botany | Year: 2011

Sap flux (Q) and trunk diameter variation (TDV) are among the most useful plant-based measurements to detect water stress and to evaluate plant water consumption. The usefulness of both methods decreases, however, when applied to species that, like olive, have an outstanding tolerance to drought and a remarkable capacity to take up water from drying soils. Evidence shows that this problem is greater in old, big trees with heavy fruit load. Our hypothesis is that the analysis of simultaneous measurements of Q and TDV made in the same trees is more useful for assessing irrigation needs in old olive orchards than the use of any of these two methods alone. To test our hypothesis, we analysed relations between Q, TDV, midday stem water potential (Ψstem), relative extractable water and atmospheric demand in an olive orchard of 38-year-old 'Manzanilla' trees with heavy fruit load. Measurements were made during one irrigation season (May-October), in fully irrigated trees (FI, 107% of the crop evapotranspiration, ETc, supplied by irrigation), and in trees under two levels of deficit irrigation (DI60, 61% ETc; DI30, 29% ETc). Time courses of Q and TDV measured on days of contrasting weather and soil water conditions were analysed to evaluate the usefulness of both methods to assess the crop water status. We calculated the daily tree water consumption (Ep) from Q measurements. For both DI treatments we calculated a signal intensity by dividing daily Ep values of each DI tree by those of the FI tree (SI-Ep). We did the same with the maximum daily shrinkage (MDS) values (SI-MDS). Neither SI-Ep nor SI-MDS rendered useful information for assessing the crop water needs. On the contrary, the daily difference for maximum trunk diameter (MXTD) between each of the DI trees and the FI tree (DMXTD) clearly indicated the onset and severity of water stress. A similar analysis with the Ep values, from which DEp values were derived, showed the effect of water stress on the water consumption of the trees. We concluded that the simultaneous use of DMXTD and DEp values provides more detailed information to assess water needs in mature olive orchards than the use of Q or TDV records alone. © 2011 Elsevier B.V.

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