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Bozic N.,University of Belgrade | Puertas J.-M.,Escola dEnginyeria | Loncar N.,University of Belgrade | Duran C.S.,Escola dEnginyeria | And 2 more authors.
Process Biochemistry | Year: 2013

In this study, a new approach for extracellular production of recombinant α-amylase in Escherichia coli was investigated. A gene encoding a highly efficient raw-starch-digesting α-amylase from Bacillus licheniformis ATCC 9945a was cloned and expressed in E. coli. The gene encoding mature α-amylase was cloned into the pDAss expression vector, and secretion of the gene product was regulated by fusion to the signal peptide of DsbA, a well-characterized E. coli periplasmic protein. E. coli BL21 (DE3) carrying pDAss vector containing amylase gene had approximately 2.5-fold higher volumetric enzyme productivity than the natural system. The recombinant enzyme showed higher efficiency for digesting diverse raw starches when compared with the native enzyme and was similar to commercial α-amylase in its ability to hydrolyze raw starches. The properties of the recombinant enzyme demonstrate the potential of the DsbA signal peptide approach for the secretory production of the fully active, industrially important recombinant enzyme. © 2013 Elsevier Ltd. All rights reserved.

Santin I.,Escola dEnginyeria | Pedret C.,Escola dEnginyeria | Vilanova R.,Escola dEnginyeria
Industrial and Engineering Chemistry Research | Year: 2015

In this paper the following new control objectives for biological wastewater treatment plants (WWTPs) have been established: to eliminate violations of total nitrogen in the effluent (Ntot,e) or ammonium and ammonia nitrogen concentration (NH) in the effluent (NHe) and at the same time handle the customary requirements of improving effluent quality and reducing operational costs. The Benchmark Simulation Model No. 1 (BSM1) is used for evaluation, and the control is based on Model Predicitive Control (MPC) and fuzzy logic. To improve effluent quality and to reduce operational costs, a hierarchical control structure is implemented to regulate the dissolved oxygen (DO) on the three aerated tanks. The high level of this hierarchical structure is developed with a fuzzy controller that adapts the DO set points of the low level based on the NH concentration in the fifth tank (NH5). The low level is composed of three MPC controllers with feedforward control (MPC + FF). For avoiding violations of Ntot,e, a second fuzzy controller is used to manipulate the external carbon flow rate in the first tank (qEC1) based on nitrate nitrogen in the fifth tank (NO5) plus NH5. For avoiding violations of NHe, a third fuzzy controller is applied to manipulate the internal recirculation flow rate (Qrin) based on NH5 and NH in the influent. Simulation results show the benefit of the proposed approach. © 2015 American Chemical Society.

Rius R.M.,Escola dEnginyeria | Talavera G.,Autonomous University of Barcelona | Carrabina J.,Autonomous University of Barcelona
2012 15th International Symposium on Antenna Technology and Applied Electromagnetics, ANTEM 2012 | Year: 2012

The emergence of wearable intelligent textile systems with sensors and actuators nodes are integrated into user clothes. Emergency units can benefit from new technologies to improve their performance and security conditions when working exposed to risk situations. In firefighters case is a the system Bluetooth Low Energy (BLE) at 2.4GHz with a watch to show the thermal stress and, in case of exceed healthy level will trigger an alarm to warn the user. In this scenario the design and study of the working conditions of an antenna is a crucial issue for ensuring the communication capabilities of the the whole system. In this work paper we present the design, development and test of a miniature flexible antenna prepared to integrate in the uniforms maintaining the radiation properties although bend or warming. We will see how to minimize the size of the antenna with a antenna PIFA on a Polyethylene Terephtalate (PET) substrate. © 2012 IEEE.

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