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Jakarta, Indonesia

Sari P.,University of Indonesia | Luviah E.,Erpour skin care | Nugraha Y.,University of Indonesia | Pawitan J.A.,University of Indonesia | And 3 more authors.
BioTechnology: An Indian Journal | Year: 2014

Aim: To compare the growth factor contents of the various PRP processing methods to get an insight on the difference in growth factor yield from the various processing methods. Experimental: PRP before and after expiry dates was collected from Indonesian Red Cross. PRP was processed by various methods to release the growth factors, and growth factor levels were measured. Growth factor levels between various kinds of processing were compared using ANOVA and those from before and after expiry date samples were compared using t test. Results: Comparison of thrombin activation, one, two, and three freeze thaw cycle(s) of all samples revealed no significant difference in PDGF-AB, EGF, and IGF levels (p> 0.05). However, there were significant differences in TGFaβ and VEGF levels between thrombin activation and freeze thaw cycles. Comparisons between samples of before and after expiry date revealed no significant differences in all growth factor levels. Conclusion: One, two, and three freeze thaw cycle(s) yielded no significant difference in most growth factor levels. © 2014 Trade Science Inc. - INDIA.

Pawitan J.A.,University of Indonesia | Suryani D.,University of Indonesia | Lilianty J.,Erpour skin care | Purwoko R.Y.,Erpour skin care | And 2 more authors.
BioTechnology: An Indian Journal | Year: 2013

Aim: to verify the potential ofVEGF supplementedDMEM-HGin inducing chondrogenic differentiation of adipose derived MSCs Experimental: Lipoaspirate derived cellswere cultured in 10%humanAB serumcontainingDMEM-HGthatwas supplemented byVEGF. The culture was observed daily, and when the cells attached on the culture vessel, the medium was changed, and further medium changes were done every 2-3 days. Cell growth was observed, and cell characteristics were identified morphologically with and without staining.After the primary culture (P0) was 70%confluent, the cells were detached and passaged. Passages were done until passage 5. For every passage, seeding number, the day of the first appearance of clones and micromasses and results of alcian blue staining for every passage were noted. Mean and standard deviation values of the day when the cells formed the first clone and when the first micromass appeared were computed. Result: VEGF supplemented human AB serum containing DMEM-HG yielded first clones on day 4.29 ± 2.05, and first micromass on day 7.69 ± 2.62, which was faster compared to the recently commercially available chondrocyte differentiation medium. Conclusion: the VEGF supplemented mediumcan be used as an alternative simple induction media for faster chondrocyte differentiation. © 2013 Trade Science Inc. - India.

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