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Gwacheon, South Korea

Schwarz B.C.,Equine Hospital | Hoven R.V.D.,Equine Hospital | Schwendenwein I.,Central Laboratory
Veterinary Journal | Year: 2012

The myeloperoxidase index (MPXI) was investigated as a diagnostic indicator of systemic inflammation in a retrospective study using data from 859 hospitalised horses. A reference interval of 8.5-10.4 for the MPXI was established. In horses with systemic inflammatory response syndrome (SIRS), the MPXI was significantly lower than in healthy horses, those with localised inflammation and those with sepsis. The MPXI in horses with sepsis was also significantly lower than in healthy animals and those with localised inflammation. Horses in the SIRS group with leucopenia, white blood cell (WBC) count within the reference interval (WRI) or leucocytosis had significantly lower MPXIs than healthy horses, those with localised inflammation and those with sepsis in the same WBC count subgroups. In horses with sepsis and WBC count WRI, the MPXI was significantly lower than in healthy horses or those with localised inflammation. MPXI is a useful complementary tool to identify horses with systemic inflammation, especially if they have WBC counts WRI. © 2010 Elsevier Ltd.

Marycz K.,Wroclaw University of Environmental and Life Sciences | Toker N.Y.,Istanbul University | Grzesiak J.,Wroclaw University of Environmental and Life Sciences | Wrzeszcz K.,Wroclaw University of Environmental and Life Sciences | Golonka P.,Equine Hospital
Journal of Animal and Veterinary Advances | Year: 2012

Naturally, occurring tendons injuries including superficial digital flexor tendinopathy are the most frequent musculoskeletal disorders in performance horses. Conventional methods of treatment with non steroidal and steroidal anti-inflammatory medicaments in majority of cases lead to scar formation, reducing the quality and efficiency of tissue regeneration. Novel approach is aimed to use cells naturally present in an organism as regeneration enhancing factor. In conducted research, the intralesional injections of autologous adipose derived stem cells combined with autologous platelet concentrate therapeutic potential was investigated in horses with 8-12 weeks duration superficial digital flexor injury with severe scaring. Collected by clinical examinations data showed positive effects of autologous, adipose-derived mesenchymal stem cells combined with autologous platelet rich plasma injections on regeneration processes in the course of superficial flexor tendon injures in horses. On the basis of ultrasound examination, it was proved that the quality of healed tissue was significantly higher in experimental group, comparing to control group. Obtained results confirmed the beneficial pro-regeneration effects of stem cells/platelet concentrate combined injections. The obtained data may also serve as valuable source of information about morphology and behaviour of fat stem cells in culture or platelets appearance. © Medwell Journals, 2012.

Ho E.N.M.,Racing Laboratory Hong Kong Jockey Club | Kwok W.H.,Racing Laboratory Hong Kong Jockey Club | Leung D.K.K.,Racing Laboratory Hong Kong Jockey Club | Riggs C.M.,Equine Hospital | And 4 more authors.
Drug Testing and Analysis | Year: 2015

Testosterone is an endogenous steroid produced primarily in the testes. Trace levels of testosterone are found in urine samples from geldings, as testosterone is also secreted by the adrenal. An international threshold of free and conjugated testosterone in urine (20ng/mL) was adopted by the International Federation of Horseracing Authorities (IFHA) in 1996 for controlling testosterone misuse in geldings. In view of the recent popularity of using blood in doping control testing, it is necessary to establish a threshold for testosterone in gelding plasma. A liquid chromatography-mass spectrometry (LC/MS) method was developed for quantifying low levels of free testosterone in gelding plasma. Based on a population study of 152 post-race plasma samples, the mean±SD concentration of plasma testosterone was determined to be 14.7±6.8pg/mL. Normal distribution could be obtained after square-root or cube-root transformation, resulting in respective tentative thresholds of 49 or 55pg/mL (corresponding to a risk factor of less than 1 in 10 000). A rounded-up threshold of 100pg/mL of free testosterone in plasma was proposed. Based on the administration of Testosterone Suspension 100 to six geldings, the same average detection time of 14days was observed in either plasma or urine using the proposed plasma threshold and the existing international urine threshold. The maximum detection time was 18days in plasma and 20days in urine. The results demonstrated the proposed plasma threshold is effective in controlling the misuse of testosterone in geldings. Similar results were subsequently obtained in Europe, and this proposed threshold was adopted by IFHA in October 2013. © 2014 John Wiley & Sons, Ltd.

Yeh J.-Y.,National Veterinary Research and Quarantine Service | Lee J.-H.,National Veterinary Research and Quarantine Service | Park J.-Y.,National Veterinary Research and Quarantine Service | Seo H.-J.,National Veterinary Research and Quarantine Service | And 8 more authors.
Vector-Borne and Zoonotic Diseases | Year: 2012

The detection of West Nile virus (WNV) in areas endemic for Japanese encephalitis virus (JEV) is complicated by the extensive serological cross-reactivity between the two viruses. A testing algorithm was developed and employed for the detection of anti-WNV antibody in areas endemic for JEV. Using this differentiation algorithm, a serological survey of poultry (2004 through 2009) and horses (2007 through 2009) was performed. Among 2681 poultry sera, 125 samples were interpreted as being positive for antibodies against JEV, and 14 were suspected to be positive for antibodies against undetermined flaviviruses other than WNV and JEV. Of the 2601 horse sera tested, a total of 1914 (73.6%) were positive to the initial screening test. Of these positive sera, 132 sera (5.1%) had been collected from horses that had been imported from the United States, where WNV is endemic. These horses had WNV vaccination records, and no significant pattern of increasing titer was observed in paired sera tests. Of the remaining 1782 positive sera 1468 sera (56.4%) were also found to contain anti-JEV antibodies, and were interpreted to be JEV-specific antibodies by the differentiation algorithm developed in this study. The remaining 314 horses (12.1%) for which a fourfold difference in neutralizing antibody titer could not be demonstrated, were determined to contain an antibody against an unknown (unidentified or undetermined) flavivirus. No evidence of WNV infections were found during the period of this study. © Copyright 2012, Mary Ann Liebert, Inc. 2012.

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