Mohanty N.,University of Veterinary and Animal Sciences |
Gulati B.R.,National Research Center on Equines |
Kumar R.,National Research Center on Equines |
Gera S.,University of Veterinary and Animal Sciences |
And 3 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2014
Mesenchymal stem cells (MSCs) isolated from umbilical cord blood (UCB) in equines have not been well characterized with respect to the expression of pluripotency and mesenchymal markers and for tenogenic differentiation potential in vitro. The plastic adherent fibroblast-like cells isolated from 13 out of 20 UCB samples could proliferate till passage 20. The cells expressed pluripotency markers (OCT4, NANOG, and SOX2) and MSC surface markers (CD90, CD73, and CD105) by RT-PCR, but did not express CD34, CD45, and CD14. On immunocytochemistry, the isolated cells showed expression of CD90 and CD73 proteins, but tested negative for CD34 and CD45. In flow cytometry, CD29, CD44, CD73, and CD90 were expressed by 96.36 ± 1.28%, 93.40 ±0.70%, 73.23 ±1.29% and 46.75 ±3.95% cells, respectively. The UCB-MSCs could be differentiated to tenocytes by culturing in growth medium supplemented with 50 ng/ml of BMP-12 by day 10. The differentiated cells showed the expression of mohawk homeobox (Mkx), collagen type I alpha 1 (Col1α1), scleraxis (Scx), tenomodulin (Tnmd) and decorin (Dcn) by RT-PCR. In addition, flow cytometry detected tenomodulin and decorin protein in 95.65±2.15% and 96.30±1.00% of differentiated cells in comparison to 11.30±0.10% and 19.45±0.55% cells, respectively in undifferentiated control cells. The findings support the observation that these cells may be suitable for therapeutic applications, including ruptured tendons in racehorses. © The Society for In Vitro Biology 2014.
Kumar D.,Equine Breeding Stud |
Jhamb D.,Equine Breeding Stud |
Jhamb D.,Government of Rajasthan |
Kumar N.,Equine Breeding Stud |
Parsad D.M.,Equine Breeding Stud
Indian Journal of Animal Sciences | Year: 2010
This study was conducted for 3 years. A total of 81 standardbred mares were used as embryo donor. Single embryo collection regime was attempted in 117 times using 3 liters of Gold Flush, Line, Euro Flush, and 95 embryos were recovered with recovery rate of 95/117=81.19% (embryo /flush). 48 embryos were transferred into synchronized recipients (+1 to -2 days) and the overall pregnancy rate at day 24 achieved was 50% (24/48). The birth of foal on 6 February 2006 through embryo transfer was reported first time in South Asia.