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Abdelmalek B.E.,Enzyme and Bioconversion Unit | Abdelmalek B.E.,University of Sfax | Sila A.,Enzyme and Bioconversion Unit | Krichen F.,Enzyme and Bioconversion Unit | And 5 more authors.
International Journal of Biological Macromolecules | Year: 2015

The characteristics, biological properties, and purification of sulfated polysaccharides extracted from squid (Loligo vulgaris) skin were investigated. Their chemical and physical characteristics were determined using X-ray diffraction and infrared spectroscopic analysis. Sulfated polysaccharides from squid skin (SPSS) contained 85.06% sugar, 2.54% protein, 1.87% ash, 8.07% sulfate, and 1.72% uronic acid. The antioxidant properties of SPSS were investigated based on DPPH radical-scavenging capacity (IC50=19.42mgmL-1), hydrogen peroxide-scavenging activity (IC50=0.91mgmL-1), and β-carotene bleaching inhibition (IC50=2.79mgmL-1) assays. ACE-inhibitory activity of SPSS was also investigated (IC50=0.14mgmL-1). Further antimicrobial activity assays indicated that SPSS exhibited marked inhibitory activity against the bacterial and fungal strains tested. Those polysaccharides did not display hemolytic activity towards bovine erythrocytes. Fractionation by DEAE-cellulose column chromatography showed three major absorbance peaks. Results of this study suggest that sulfated polysaccharides from squid skin are attractive sources of polysaccharides and promising candidates for future application as dietary ingredients. © 2014 Elsevier B.V.

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