Enzyme and Bioconversion Unit

Sfax, Tunisia

Enzyme and Bioconversion Unit

Sfax, Tunisia
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Ben Abdallah Kolsi R.,University of Sfax | Ben Salah H.,University of Sfax | Jardak N.,University of Sfax | Chaaben R.,Biochemistry Laboratory | And 6 more authors.
Biomedicine and Pharmacotherapy | Year: 2017

This new study aimed to evaluate for the first time the effect of Cymodocea nodosa extract (CNE) on α-amylase activity, hyperglycemia and diabetes complications in the alloxan-induced diabetic rats. The in vitro evaluation and oral administration of CNE to surviving diabetic rats inhibited key enzyme related to hyperglycemia as α-amylase, helped to protect the β cells of the rats from death and damage confirmed by oral glucose test tolerance (OGTT), which leads to decrease in blood glucose level by 49% as compared to untreated diabetic rats. The CNE also decreased the triglyceride, low density lipoprotein (LDL) cholesterol and total cholesterol rates in the plasma of diabetic rats by 46%, 35%, and 21%, respectively, and increased the high density lipoprotein (HDL) cholesterol level by 36%, which helped maintain the homeostasis of blood lipid. When compared to those of the untreated diabetic rats, the superoxide dismutase, catalase, and glutathione peroxidase levels in the pancreas, liver and kidney of the rats treated with this supplement were also enhanced significantly. Moreover, a significant decrease was observed in the lipid peroxidation level in the tested organs of diabetic rats after CNE administration. This positive effect of CNE was confirmed by histological study. Overall, the findings presented in this study demonstrate that CNE has both a promising potential with a valuable hypoglycemic and hypolipidemic functions. © 2017 Elsevier Masson SAS


Kolsi R.B.A.,University of Sfax | Salah H.B.,University of Sfax | Jardak N.,Research Unit of anatomy | Chaaben R.,Biochemistry Laboratory | And 8 more authors.
Carbohydrate Polymers | Year: 2017

A sulphated polysaccharide from brown algae Sargassum vulgare (SVSP) was extracted and examined with respect to chemical, structural characterization and hypolipidemic effects. SVSP consisted mainly of sulphate and total sugars with low levels of lipids and proteins. Its structure was studied by nuclear magnetic resonance (RMN), gas chromatography-mass spectrometry (GC–MS), infra-red spectroscopic, differential scanning calorimetry and X-ray diffraction analysis. Allowing us therefore to revealed that SVSP was composed of glucose, rhamnose, xylose, galactose, mannose and arabinose with XRD pattern that was typical for a semi-crystalline polymer and complexities of the spectra reflected its homogeneous structure. The administration of SVSP to obese rats is effective in lowering the body weight and inhibiting the lipase activity leading to notable regulation of lipid profile, increasing the activities of antioxidant enzymes, limiting lipid peroxidation; and protects liver-kidney functions proved by a decrease in the levels of toxicity parameters in blood, confirmed by histological study. © 2017


Abdelmalek B.E.,Enzyme and Bioconversion Unit | Abdelmalek B.E.,University of Sfax | Sila A.,Enzyme and Bioconversion Unit | Krichen F.,Enzyme and Bioconversion Unit | And 5 more authors.
International Journal of Biological Macromolecules | Year: 2015

The characteristics, biological properties, and purification of sulfated polysaccharides extracted from squid (Loligo vulgaris) skin were investigated. Their chemical and physical characteristics were determined using X-ray diffraction and infrared spectroscopic analysis. Sulfated polysaccharides from squid skin (SPSS) contained 85.06% sugar, 2.54% protein, 1.87% ash, 8.07% sulfate, and 1.72% uronic acid. The antioxidant properties of SPSS were investigated based on DPPH radical-scavenging capacity (IC50=19.42mgmL-1), hydrogen peroxide-scavenging activity (IC50=0.91mgmL-1), and β-carotene bleaching inhibition (IC50=2.79mgmL-1) assays. ACE-inhibitory activity of SPSS was also investigated (IC50=0.14mgmL-1). Further antimicrobial activity assays indicated that SPSS exhibited marked inhibitory activity against the bacterial and fungal strains tested. Those polysaccharides did not display hemolytic activity towards bovine erythrocytes. Fractionation by DEAE-cellulose column chromatography showed three major absorbance peaks. Results of this study suggest that sulfated polysaccharides from squid skin are attractive sources of polysaccharides and promising candidates for future application as dietary ingredients. © 2014 Elsevier B.V.

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