Farmingdale, NY, United States
Farmingdale, NY, United States

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Patent
Enzo Life Sciences | Date: 2016-10-05

This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.


Patent
Enzo Life Sciences | Date: 2017-03-15

The present invention provides a composition of matter comprising: (a) a first part comprising at least one first nucleic acid primer that comprises (i) at least one first energy transfer element; and (ii) a nucleic acid sequence which is complementary to a nucleic acid sequence in at least a portion of a nucleic acid of interest; (b) a second part comprising at least one second nucleic acid primer that comprises: (i) at least one second energy transfer element; and (ii) a nucleic acid sequence which is identical to a nucleic acid sequence in at least a portion of said nucleic acid of interest; wherein said first nucleic acid primer does not comprise said second energy transfer element, and wherein said second nucleic acid primer does not comprise said first energy transfer element; wherein the first energy transfer element is capable of acting as an energy donor and the second energy transfer element is capable of acting as an energy acceptor, or the first energy transfer element is capable of acting as acting as an energy acceptor and the second energy transfer element is capable of acting as an energy donor; and wherein the sequences of the first nucleic acid primer and the second nucleic acid primer are selected such that if the nucleic acid of interest is present in the sample, said first and second nucleic acid primers will be incorporated into different complementary strands of a double stranded amplicon in sufficient proximity for energy transfer to occur between the first energy transfer element and the second energy transfer element of the incorporated primers; (c) a sample suspected of containing said nucleic acid of interest; and (d) reagents for carrying out nucleic acid strand extension.


Patent
Enzo Life Sciences | Date: 2017-01-10

Provided are methods, kits and systems for simultaneously profiling of global reactive species and selected reactive species, such as global and specific reactive oxygen species (ROS), reactive nitrogen species (RNS), reactive halogen species (RHS) or combinations thereof through multiplexed fluorescence detection of three or more compatible indicator probes in live cells or subcellular organelles.


Patent
Enzo Life Sciences | Date: 2017-01-26

Provided is a compound having the structure: (SIG)-(SI-MOD)_(m ) where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.


Patent
Enzo Life Sciences | Date: 2016-09-01

The disclosure relates to methods for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid sample. The disclosure further relates to kits for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid.


Patent
Enzo Life Sciences | Date: 2016-03-30

Provided are assays useful for detecting and monitoring autophagy and phospholipidosis, including the progression of lysosomal storage diseases. Drugs and treatments for lysosomal storage diseases can be monitored for effectiveness in lysosomal storage disease conditions. Drug candidates and suspected toxic agents can also be screened for toxicity to cells, tissues and organs. Also provided are methods for distinguishing between phospholipidosis activators and autophagy pathway perturbation agents.


Patent
Enzo Life Sciences | Date: 2016-03-09

Provided are assays useful for detecting and monitoring autophagy and phospholipidosis, including the progression of lysosomal storage diseases. Drugs and treatments for lysosomal storage diseases can be monitored for effectiveness in lysosomal storage disease conditions. Drug candidates and suspected toxic agents can also be screened for toxicity to cells, tissues and organs. Also provided are methods for distinguishing between phospholipidosis activators and autophagy pathway perturbation agents.


Patent
Enzo Life Sciences | Date: 2016-06-28

Provided are methods and compositions which are useful for separating, isolating, detecting, and quantifying compounds of interest which have been modified chemically, enzymatically or catalytically from other compounds which have not been so modified. The modifications may take the form of functional groups which are gained, lost or retained by the compounds of interest.


Patent
Enzo Life Sciences | Date: 2016-03-30

This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided. Paneling and multiplex analyses of more than one nucleic acid analyte using one sample are also provided.


Patent
Enzo Life Sciences | Date: 2016-05-17

Provided are dyes and compositions which are useful in a number of applications, such as the detection and monitoring protein aggregation, kinetic studies of protein aggregation, neurofibrillary plaques analysis, evaluation of protein formulation stability, and analysis of molecular chaperone activity.

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