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Murviel-lès-Montpellier, France

Muller M.,CNRS Microbial Ecology | Muller M.,French National Institute for Agricultural Research | Muller M.,Envolure SAS | Jimenez J.,Veolia | And 7 more authors.
Waste Management

The design and management of anaerobic digestion of sewage sludge (SS) require a relevant characterisation of the sludge organic matter (OM). Methods currently used are time-consuming and often insufficiently informative. A new method combining chemical sequential extractions (CSE) with 3D fluorescence spectroscopy was developed to provide a relevant SS characterisation to assess both OM bioaccessibility and complexity which govern SS biodegradability. CSE fractionates the sludge OM into 5 compartments of decreasing accessibility. First applied on three SS samples with different OM stability, fractionation profiles obtained were in accordance with the latter. 3D fluorescence spectroscopy revealed that the bioaccessible compartments were mainly constituted of simple and easily biodegradable OM while the unaccessible ones were largely made of complex and refractory OM. Then, primary, secondary and anaerobically digested sludge with different biodegradabilities were tested. Complexity revealed by 3D fluorescence spectroscopy was linked with biodegradability and chemical accessibility was correlated with sludge bioaccessibility. © 2014 Elsevier Ltd. Source

Sykora S.,University of Science and Arts of Iran | Cumbo A.,INOFEA GmbH | Belliot G.,National Reference Center for Enteric Viruses | Pothier P.,National Reference Center for Enteric Viruses | And 4 more authors.
Chemical Communications

Functional recognition imprints of virus-like particles, at the surface of silica particles, were generated following a strategy based on protein-templated polycondensation of organosilanes. © The Royal Society of Chemistry 2015. Source

Muller M.,Envolure SAS | Bouguelia S.,Envolure SAS | Goy R.-A.,Envolure SAS | Yoris A.,Envolure SAS | And 6 more authors.
Environmental Science and Pollution Research

BOD5 dates back to 1912 when the Royal Commission decided to use the mean residence time of water in the rivers of England, 5 days, as a standard to measure the biochemical oxygen demand. Initially designed to protect the quality of river waters from extensive sewage discharge, the use of BOD5 has been quickly extended to waste water treatment plants (WWTPs) to monitor their efficiency on a daily basis. The measurement has been automatized but remains a tedious, time- and resource-consuming analysis. We have cross-validated a surrogate BOD5 method on two sites in France and in the USA with a total of 109 samples. This method uses a fluorescent redox indicator on a 96-well microplate to measure microbial catabolic activity for a large number of samples simultaneously. Three statistical tests were used to compare surrogate and reference methods and showed robust equivalence. © 2014, Springer-Verlag Berlin Heidelberg. Source

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