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Cho K.,Environmental Biology Division | Shibato J.,Environmental Biology Division | Kubo A.,Environmental Biology Division | Kohno Y.,Environmental Science Research Laboratory | And 4 more authors.
Plant signaling & behavior

High ozone (O3) concentrations not only damage plant life but also cause considerable losses in plant productivity. To screen for molecular factors usable as potential biomarkers to identify for O3-sensitive and -tolerant lines and design O3 tolerant crops, our project examines the effects of O3 on rice, using high-throughput omics approaches. In this study, we examined growth and yield parameters of 4 rice cultivars fumigated for a life-time with ambient air (mean O3: 31.4-32.7 ppb) or filtered air (mean O3: 6.6-8.3 ppb) in small open-top chambers (sOTCs) to select O3-sensitive (indica cv Takanari) and O3-tolerant (japonica cv Koshihikari) cultivars for analysis of seed transcriptomes using Agilent 4 × 44K rice oligo DNA chip. Total RNA from dry mature dehusked seeds of Takanari and Koshihikari cultivars was extracted using a modified protocol based on cethyltrimethylammonium bromide extraction buffer and phenol-chloroform-isoamylalcohol treatment, followed by DNA microarray analysis using the established dye-swap method. Direct comparison of Koshihikari and Takanari O3 transcriptomes in seeds of rice plants fumigated with ambient O3 in sOTCs successfully showed that genes encoding proteins involved in jasmonic acid, GABA biosynthesis, cell wall and membrane modification, starch mobilization, and secondary metabolite biosynthesis are differently regulated in sensitive cv Takanari and tolerant cv Koshihikari. MapMan analysis further mapped the molecular factors activated by O3, confirming Takanari is rightly classified as an O3 sensitive genotype. Source

Sakai K.,Meiji University | Ono H.,Environmental Science Research Laboratory | Ito M.,Nishimatsu Construction
Proceedings of BS 2013: 13th Conference of the International Building Performance Simulation Association

This paper describes the measurement of wind velocity and pressure difference between that inside and outside of a single sliding door at open-close operation, and describes CFD analysis using measured pressure difference, aiming at the development of a CFD analysis approach considering opening and closing of a sliding door. Comparison with measurements verified that the analysis can reproduce unsteady phenomena observed in measurement in general. Copyright © 2011 by IPAC'11/EPS-AG. Source

Wakabayashi K.,Tokyo University of Marine Science and Technology | Sato R.,Tokyo University of Marine Science and Technology | Ishii H.,Tokyo University of Marine Science and Technology | Akiba T.,Japan National Institute of Advanced Industrial Science and Technology | And 2 more authors.

The complete larval development of a slipper lobster, Ibacus novemdentatus, from newly hatched phyllosomas to juveniles fed only jellyfish, was successfully achieved. Most larvae passed through 6 phyllosomal and 1 nisto stages. The phyllosomal duration was shorter than that reported in previous studies; notably the 7th larval stage was skipped. This is the first report on the complete phyllosomal development with jellyfish as the sole food source. Moreover, the phyllosomas were reared under either closed recirculating or static conditions, where the shortest duration of phyllosomas was 44 and 41. days, respectively. Finally, 12.5% of phyllosomas in recirculating condition and 36.1% in static condition metamorphosed into nistos. We conclude that phyllosomas of I. novemdentatus could metamorphose into nisto in a labor-saving closed recirculating system furnished with ultraviolet sterilization and foam fractionation. The results indicate that mass production of phyllosomas in I. novemdentatus, reared solely on jellyfish in recirculating system, is feasible. © 2011 Elsevier B.V.. Source

Fukada K.,Shinryo Corporation | Sakai K.,Meiji University | Ono H.,Environmental Science Research Laboratory
14th International Conference of IBPSA - Building Simulation 2015, BS 2015, Conference Proceedings

In this paper, we compared measurement results with the CFD analysis results using several of modeling methods for a four-way cassette type outlet. As a result, we compiled possible phenomenon in the case of inappropriate modeling methods. For example, the influence of the Coanda effect was overestimated than measurement results when we placed the outlet to the height that was the same as a ceiling and set diagonal airflow condition. Finally we described a modeling method of CFD parts which is appropriate for business and the design phase even if we carry out CFD analysis using a coarse mesh. Source

Hirano S.-I.,Environmental Science Research Laboratory | Nagaok T.,Environmental Science Research Laboratory | Ise K.,Japan Atomic Energy Agency | Amano Y.,Japan Atomic Energy Agency | Matsumoto N.,Environmental Science Research Laboratory
Zairyo to Kankyo/ Corrosion Engineering

To obtain the knowledge about the corrosion ability and its mechanism as a target the soil environment microorganisms, lake mud was cultured with metallic iron. As a result, corrosion of carbon steel was observed with sulfate reduction and methane producing activity in brackish medium with lactate as substrate. The next-generation sequencing technologies revealed microbial community varies greatly between environmental samples (inoculated sample) and cultured sample. Particularly, Desulfovibrio species and Clostridia class, that sulfate reducing ability have been reported, became dominant. Inhibition test of sulfate reducing bacteria and methane producing archaea suggested that sulfate reducing bacteria plays a major role for this corrosion, and methane producing archaea enhanced corrosion activity by the coexistence of sulfate reducing bacteria. Source

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