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Zhang C.,Environmental Protection Research Institute of Light Industry | Zhang C.,University of California at Riverside | Larry Li B.,University of California at Riverside
Shengtai Xuebao/ Acta Ecologica Sinica

On the basis of field visits of the ecological reclamation projects in the United States abandoned coal mine, along with the relevant literature, we reviewed the current practices in the United States on their management, funding usage, and the technical route of the ecological reclamation and restoration of mine waste. Engineering measures of ecological restoration for abandoned coal mine were described with several examples. A new idea named "Geomorphic Reclamation" was introduced as a fundamental shift in the current theory and practice of ecological restoration of mining sites, which is an integrated approach, learning from Nature, along with the combination of geomorphic, topological, soil, ecological, hydrologic and climate/weather conditions, for the planning, design and implication of ecological reclamation and restoration. We address the potential of Geomorphic Reclamation in China and outline how such positive and potentially far-reaching impact approach can be used in Chinese mining restoration and management. Source

Pan Y.,CAS Institute of Biophysics | Jin J.-H.,Environmental Protection Research Institute of Light Industry | Yu Y.,CAS Tianjin Institute of Industrial Biotechnology | Wang J.,CAS Institute of Biophysics

The reversible acetylation of proteins plays a key role in regulating biological processes, including chromatin remodeling, progression of the cell cycle, and actin nucleation. Human peroxiredoxin 1(hPrx1), one of the most abundant proteins in the cytoplasm, has been shown to be acetylated in human liver-carcinoma tissues. However, little is known about what function(s) the acetylation serves for hPrx1. Herein, using the method of genetic code expansion, we incorporated Nε-acetyllysine (AcK) site-specifically into hPrx1. Our data showed that acetylation the K 27 residue promotes oligomerization of hPrx1 at low concentrations. In addition, K27-acetylated hPrx1(hPrx1-AcK27) exhibited greatly enhanced chaperone activity (e.g. protecting the protein malate dehydrogenase (MDH) from thermally induced aggregation and assisting the refolding of denatured citrate synthase (CS)). These findings suggest that the site-specific acetylation of hPrx1 may change its biological role in response to environmental changes. Time for a substitution: The non-canonical amino acid N ε-acetyllysine was incorporated into human peroxiredoxin 1 (hPrx1) through amber codon suppression to study the function of lysine acetylation in modulating the chaperone activity of hPrx1. This site-specific acetylation was found to affect both hPrx1 oligomerization and its protein-refolding activity. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

Meng Y.-T.,Beijing Radiation Center | Jin J.-H.,Environmental Protection Research Institute of Light Industry
2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings

In this study, nitrogen ion implantation was used to mutate a pyrene-degrading bacterium, Gordona sp. hbs1, which was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil. The pyrene degradation characteristics of hbs1, including isotherm and pH effect were observed. 200 mg/L pyrene, 53.78% was degraded by hbs1, equals to 10.75 mg/L. The optimum initial pH for pyrene degradation was 7 to 7.5. The mutagenesis was conducted by 30 KeV nitrogen ion implantation with the doses of 5×10 12, 1×10 13, 2×10 13, 4×10 13, 5×10 13, 1×10 14, 2×10 14, 5×10 14 ions/cm 2. The results showed that the optimum dose was 1×10 14 ions/cm 2. Three highly pyrene degrading strains, hbs1-m11, hbs1-m23 and hbs1-m48 were obtained and their increased efficiencies were 18.72%, 10.71% and 13.10%, respectively. Furthermore, pyrene degrading rate of hbs1-m11 was higher than hbs1. These results imply that ion implantation could be a potential technology in environmental bioremediation. © 2011 IEEE. Source

Xu L.,Environmental Protection Research Institute of Light Industry
Wei sheng wu xue bao = Acta microbiologica Sinica

The aim of this study was to isolate high-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs) degrading bacterial strains, and to study their degradation potential. We used sublimation method to enrich and isolate the degrading bacteria from coking plant samples. Morphological properties, the sequence homology of 16S rRNA and gyrb genes were used to identify the isolated strains. GC-MS was used to analyze the degradation potential against some HMW-PAHs. An HMW-PAHs degrading bacterium,HBS1, was obtained. HBS1 could use several HMW-PAHs such as pyrene, benzoanthracene, benzopyrene, chyrsene, indeno[ 1,2,3-cd] pyrene, benzo[g,h,i]perylene and fluoranthene as sole carbon source for growth. HBS1 was identified as Gordonia sp., based on the high sequence similarities (more than 99%) of both 16S rRNA gene and gyrb gene to those of Gordonia amicalis. When the initial concentration of pyrene was 50 mg/L, HBS1 could consume 97% of the pyrene in 17 days. One fragment of the dioxygenase gene was obtained by PCR with size about 300 bp, which was closest to the counterpart from Mycobacterium sp. with 93.8% similarity. We isolated a strain HBS1 from seriously PAHs-polluted soils and identified as a Gordonia sp. The isolate had the degradation potential of high-molecular weight polycyclic aromatic hydrocarbons. Source

Liu Y.,CAS Institute of Microbiology | Jin J.-H.,Environmental Protection Research Institute of Light Industry | Liu Y.-H.,CAS Institute of Microbiology | Zhou Y.-G.,CAS Institute of Microbiology | Liu Z.-P.,CAS Institute of Microbiology
International Journal of Systematic and Evolutionary Microbiology

A Gram-negative, strictly aerobic and heterotrophic, non-spore-forming bacterial strain, designated LM22T, was isolated from activated sludge of a sequencing batch reactor for the treatment of malachite green effluent. Cells of strain LM22T were slightly curved to straight rods (0.3-0.5x0.6-1.0 μm) and motile by a single polar flagellum. Strain LM22T was negative for oxidase and catalase activities and phototrophic growth. An internal membrane system and bacteriochlorophyll a were absent. Growth occurred at 20-40 °C (optimum 30-35 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). Strain LM22T did not require NaCl for growth and tolerated up to 2.0% NaCl (optimum 0.5 %). The major ubiquinone was Q-10. The major fatty acids (>10% of the total) were C18 : 1ω7c (32.9 %), C19 : 0 cyclo ω8c (18.7 %), C16 : 0 (12.1 %) and C16 : 0 2-OH (10.5 %). Phylogenetic analysis of 16S rRNA gene sequences showed that Inquilinus limosus AU0476T was the closest relative (90.4% 16S rRNA gene sequence similarity). The DNA G+C content was 65.6 mol%. On basis of phenotypic, chemotaxonomic and phylogenetic data, strain LM22T was considered to represent a novel genus and species of the family Rhodospirillaceae, for which the name Dongia mobilis gen. nov., sp. nov. is proposed. The type strain of Dongia mobilis is LM22T (=CGMCC 1.7660T =JCM 15798T). © 2010 IUMS. Source

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