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Pan Y.,CAS Institute of Biophysics | Jin J.-H.,Environmental Protection Research Institute of Light Industry | Yu Y.,CAS Tianjin Institute of Industrial Biotechnology | Wang J.,CAS Institute of Biophysics
ChemBioChem | Year: 2014

The reversible acetylation of proteins plays a key role in regulating biological processes, including chromatin remodeling, progression of the cell cycle, and actin nucleation. Human peroxiredoxin 1(hPrx1), one of the most abundant proteins in the cytoplasm, has been shown to be acetylated in human liver-carcinoma tissues. However, little is known about what function(s) the acetylation serves for hPrx1. Herein, using the method of genetic code expansion, we incorporated Nε-acetyllysine (AcK) site-specifically into hPrx1. Our data showed that acetylation the K 27 residue promotes oligomerization of hPrx1 at low concentrations. In addition, K27-acetylated hPrx1(hPrx1-AcK27) exhibited greatly enhanced chaperone activity (e.g. protecting the protein malate dehydrogenase (MDH) from thermally induced aggregation and assisting the refolding of denatured citrate synthase (CS)). These findings suggest that the site-specific acetylation of hPrx1 may change its biological role in response to environmental changes. Time for a substitution: The non-canonical amino acid N ε-acetyllysine was incorporated into human peroxiredoxin 1 (hPrx1) through amber codon suppression to study the function of lysine acetylation in modulating the chaperone activity of hPrx1. This site-specific acetylation was found to affect both hPrx1 oligomerization and its protein-refolding activity. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Sun G.-D.,CAS Institute of Microbiology | Sun G.-D.,Tsinghua University | Jin J.-H.,Environmental Protection Research Institute of Light Industry | Xu Y.,CAS Institute of Microbiology | And 3 more authors.
International Biodeterioration and Biodegradation | Year: 2014

A high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs)-degrading strain, designed P10, was isolated from PAHs contaminated soil. Strain P10 was preliminarily identified as Kocuria sp. based on the analysis of 16S rRNA gene sequence. Degradation of individual PAHs was investigated in basal salt medium using PAH as sole carbon and energy source. The results showed that strain P10 could degrade several PAHs including HMW-PAHs, such as fluorene, fluoranthene and indeno[1,2,3-cd]pyrene with degrading percentages of 83.2%, 76.5% and 59.6%, respectively, after incubation for 14 days at 25°C. Bioremediation experiments of HMW-PAHs heavily contaminated soil were also carried out using strain P10 as inoculum in weathered plots under natural conditions. The results indicated that strain P10 could significantly enhance the removal of HMW-PAHs from the contaminated soil. The removal percentages were increased by about 2-folds for HMW-PAHs in comparison to the non-inoculated one. © 2014 Elsevier Ltd.

Xu Y.,CAS Institute of Microbiology | Xu Y.,University of Chinese Academy of Sciences | Sun G.-D.,CAS Institute of Microbiology | Sun G.-D.,Tsinghua University | And 5 more authors.
Journal of Hazardous Materials | Year: 2014

Bioremediation of an aged and heavily contaminated soil was performed using microbial remediation, phytoremediation, and microbial/phytoremediation. The removal efficiency of polycyclic aromatic hydrocarbons (PAHs) was in the order microbial/phytoremediation. >. microbial remediation. ≈. phytoremediation. >. control. The removal percentage of microbial/phytoremediation (69.6%) was twice that of control. Kocuria sp. P10 significantly enhanced PAH removal (P<. 0.05) and ryegrass growth (P<. 0.01). Dehydrogenase activity increased steadily and was negatively correlated with total PAH content. Successional changes in soil microbial communities were also detected by pyrosequencing. The results indicated that biodiversity of the soil bacterial community gradually increased with time and was slightly lower in control, as indicated by operational taxonomic unit (OTU) numbers and Shannon-Wiener indices. Proportions of Betaproteobacteria and Gammaproteobacteria were consistently high in all groups. Actinobacteridae were initially predominant (>37.8%) but rapidly decreased to <4%. The proportions of Acidobacteria increased greatly and this increase was positively correlated with PAH removal. These findings indicate a healthy ecological progression and a role of Acidobacteria as an indicator of the process. This study provides new insights into the dynamics of community structure during bioremediation process and a possible basis for ecological assessment for bioremediation on a large scale. © 2013 Elsevier B.V.

Sun G.-D.,CAS Institute of Microbiology | Sun G.-D.,Tsinghua University | Xu Y.,CAS Institute of Microbiology | Jin J.-H.,Environmental Protection Research Institute of Light Industry | And 4 more authors.
Journal of Hazardous Materials | Year: 2012

This study aims at the remediation of heavily PAH-contaminated soil containing 375. mg of total PAHs per kilogram dry soil. Pilot scale bioremediation experiments were carried out by three approaches with contaminated soil from abandoned sites of Beijing Coking Plant using outdoor pot trials. The first approach was bioaugmentation with a bacterial strain which degrades PAH and produces bioemulsifier, the second approach comprised of biostimulation of indigenous microorganisms with supplementing nutrients and the last approach involved the combination of both biostimulation and bioaugmentation. An on-site land farming group was set as a control in which the total PAHs and 4-6 ring-PAHs were reduced by 23.4% and 10.1%, respectively after 175 days. Meanwhile, in the first approach group, the total PAHs and 4-6 ring-PAHs were reduced by 26.82% and 35.36%, respectively; in the second approach group both percentages were 33.9% and 11.0%, respectively; while in the third approach group, these pollutants were reduced by 43.9% and 55.0%, respectively. The results obtained suggested that biostimulation and bioaugmentation combined could significantly enhance the removal of PAHs in the contaminated soil. © 2012 Elsevier B.V.

Wang L.,Shanxi Agricultural University | Han Y.,Shanxi Agricultural University | Zhang C.,Environmental Protection Research Institute of Light Industry | Pei Z.,Shanxi Agricultural University
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2011

The main obstacles to restoration of the vegetation and ecological reconstruction of abandoned mines are from the special and poor physical and chemical properties of the land, particularly to gangue piles. Vegetation is based on soil and also affects soil formation, nutrient cycling and development. Study of nutrient change in soil is important to understand soil fertility and mechanism of nutrient cycling. The study of re-vegetation for coal gangue piles has mainly focused on growth of young forest, soil matrix improvement, vegetation restoration patterns, vegetation dynamics and vegetation restoration techniques and mechanisms, etc. However, there are no many studies on eco-environmental effects of vegetation on gangue piles. Study of the relationship between environment and vegetation on gangue piles is helpful to reveal the stability and dynamic development of existing ecosystem, and to build up the foundation for vegetation regulation on gangue piles. In this study, different reclaimed lands from 280 gangue piles of No. 3 coal mine of Yangquan Coal Industry Group in Shanxi Province were included. The effect of different vegetation restoration patterns were analyzed on reclaimed soil and weathered coal gangue. The soil, physical and chemical properties of weathered coal gangue and heavy metals under 6 different vegetation types (platation of Robinia pseudoacacia L., mixed plantation of Robinia pseudoacacia L. and Caragana intermedia, mixed plantation of Ulmus pumila L. and Amorpha fruticosa L., mixed plantation of Platycladus orientalis (L.) Franco and Robinia pseudoacacia L., Vitex negundo var. heterophylla and grass vegetation of Medicago sativa L.) on coal gangue piles were compared. The results showed: (1) The vegetation, particularly the tree plantation on coal gangue piles significantly influenced the soil bulk density, soil water holding capacity and porosity. As the vegetation was recovered, the nutrient content in the soil significantly increased. Soil nutrient content varied with the difference between vegetation restoration types. The mixed plantation of R. pseudoacacia and C. intermedia was much better than other vegetation types. The order of different vegetation types according to their positive effects on soil bulk density and mechanical components of coal gangue were: coniferous> mixed shrub and tree forest> broad-leaf tree pure forest> shrub> grass; (2) The vegetation order according to the accumulation of organic matter and total nitrogen were: mixed plantation of P. orientalis and R. pseudoacacia > R. pseudoacacia plantation > mixed plantation of R. pseudoacacia and C. intermedia > mixed plantation of U. pumila and A. fruticosa > V. negundo var. heterophylla plantation > Grass of M. sativa. Total P content under V. negundo var. heterophylla plantation on weathered gangue piles was the highest. V. negundo var. heterophylla plantation and mixed plantation of P. orientalis and R. pseudoacacia were good for quick P accumulation, and mixed plantation of U. pumila and A. fruticosa for quick K accumulation; (3) Planting of vegetation could reduce the heavy metal content in weathered gangue. Mixed plantation of U. pumila and A. fruticosa was better in reducing excessive heavy metal Cd, and followed by mixed coniferous forest (mixed plantation of P. orientalis and R. pseudoacacia). In reduction of excessive heavy metal As, the mixed plantation of P. orientalis and R. pseudoacacia was better than the other vegetation types, and could control the As level lower than the national standard.

Meng Y.-T.,Beijing Radiation Center | Jin J.-H.,Environmental Protection Research Institute of Light Industry
2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings | Year: 2011

In this study, nitrogen ion implantation was used to mutate a pyrene-degrading bacterium, Gordona sp. hbs1, which was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil. The pyrene degradation characteristics of hbs1, including isotherm and pH effect were observed. 200 mg/L pyrene, 53.78% was degraded by hbs1, equals to 10.75 mg/L. The optimum initial pH for pyrene degradation was 7 to 7.5. The mutagenesis was conducted by 30 KeV nitrogen ion implantation with the doses of 5×10 12, 1×10 13, 2×10 13, 4×10 13, 5×10 13, 1×10 14, 2×10 14, 5×10 14 ions/cm 2. The results showed that the optimum dose was 1×10 14 ions/cm 2. Three highly pyrene degrading strains, hbs1-m11, hbs1-m23 and hbs1-m48 were obtained and their increased efficiencies were 18.72%, 10.71% and 13.10%, respectively. Furthermore, pyrene degrading rate of hbs1-m11 was higher than hbs1. These results imply that ion implantation could be a potential technology in environmental bioremediation. © 2011 IEEE.

Liu Y.,CAS Institute of Microbiology | Jin J.-H.,Environmental Protection Research Institute of Light Industry | Liu Y.-H.,CAS Institute of Microbiology | Zhou Y.-G.,CAS Institute of Microbiology | Liu Z.-P.,CAS Institute of Microbiology
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

A Gram-negative, strictly aerobic and heterotrophic, non-spore-forming bacterial strain, designated LM22T, was isolated from activated sludge of a sequencing batch reactor for the treatment of malachite green effluent. Cells of strain LM22T were slightly curved to straight rods (0.3-0.5x0.6-1.0 μm) and motile by a single polar flagellum. Strain LM22T was negative for oxidase and catalase activities and phototrophic growth. An internal membrane system and bacteriochlorophyll a were absent. Growth occurred at 20-40 °C (optimum 30-35 °C) and pH 6.0-10.0 (optimum pH 7.0-7.5). Strain LM22T did not require NaCl for growth and tolerated up to 2.0% NaCl (optimum 0.5 %). The major ubiquinone was Q-10. The major fatty acids (>10% of the total) were C18 : 1ω7c (32.9 %), C19 : 0 cyclo ω8c (18.7 %), C16 : 0 (12.1 %) and C16 : 0 2-OH (10.5 %). Phylogenetic analysis of 16S rRNA gene sequences showed that Inquilinus limosus AU0476T was the closest relative (90.4% 16S rRNA gene sequence similarity). The DNA G+C content was 65.6 mol%. On basis of phenotypic, chemotaxonomic and phylogenetic data, strain LM22T was considered to represent a novel genus and species of the family Rhodospirillaceae, for which the name Dongia mobilis gen. nov., sp. nov. is proposed. The type strain of Dongia mobilis is LM22T (=CGMCC 1.7660T =JCM 15798T). © 2010 IUMS.

Sun R.,CAS Institute of Microbiology | Jin J.,Environmental Protection Research Institute of Light Industry | Sun G.,CAS Institute of Microbiology | Liu Y.,CAS Institute of Microbiology | Liu Z.,CAS Institute of Microbiology
Journal of Environmental Sciences | Year: 2010

Inoculation with efficient microbes had been proved to be the most important way for the bioremediation of polluted environments. For the treatment of abandoned site of Beijing Coking Chemical Plant contaminated with high level of high-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs), a bacterial consortium capable of degrading HMW-PAHs, designated 1-18-1, was enriched and screened from HMW-PAHs contaminated soil. Its degrading ability was analyzed by high performance liquid chromatography (HPLC), and the community structure was investigated by construction and analyses of the 16S rRNA gene clone libraries (A, B and F) at different transfers. The results indicated that 1-18-1 was able to utilize pyrene, fluoranthene and benzo[a]pyrene as sole carbon and energy source for growth. The degradation rate of pyrene and fluoranthene reached 82.8% and 96.2% after incubation for 8 days at 30°C, respectively; while the degradation rate of benzo[a]pyrene was only 65.1% after incubation for 28 days at 30C. Totally, 108, 100 and 100 valid clones were randomly selected and sequenced from the libraries A, B, and F. Phylogenetic analyses showed that all the clones could be divided into 5 groups, Bacteroidetes, α-Proteobacteria, Actinobacteria, βProteobacteria and γ-Proteobacteria. Sequence similarity analyses showed total 39 operational taxonomic units (OTUs) in the libraries. The predominant bacterial groups were α-Proteobacteria (19 OTUs, 48.7%), γ-Proteobacteria (9 OTUs, 23.1%) and β-Proteobacteria (8 OTUs, 20.5%). During the transfer process, the proportions of α-Proteobacteria and β-Proteobacteria increased greatly (from 47% to 93%), while γ-Proteobacteria decreased from 32% (library A) to 6% (library F); and Bacteroidetes group disappeared in libraries B and F. © 2010 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.

Li X.,Environmental Protection Research Institute of Light Industry | Song Y.,Environmental Protection Research Institute of Light Industry | Liu Y.-B.,Environmental Protection Research Institute of Light Industry
Huanjing Kexue/Environmental Science | Year: 2014

Stabilization of Pb, Cd and Zn spiked soil by using lime-stabilized sewage sludge (LSS) as amendment was investigated in this study, and the effectiveness was evaluated by using leaching tests (TCLP, SPLP and de-ionized water) and modified BCR sequential extraction procedure. The results of TCLP indicated that the concentrations of heavy metals in TCLP leachate reduced significantly with the increase of the mass percentage of the LSS and the leaching reduction rates were as high as 99.54% for Zn, 99.60% for Pb, 99.85% for Cd at 40% of LSS addition. When evaluated by SPLP and de-ionized water leaching method, the concentrations of Zn and Pb in leachate decreased obviously at 10% and 20% of LSS additions, but subsequently increased at 30% and 40% because of redissolution of Zn and Pb at strong base condition. After pH value of LLS-stabilized soil was adjusted by ferrous sulfate and phosphoric acid for recovering soil plantation function, the pH value of the soil decreased effectively, in the meantime promoting the stabilization effectiveness of Pb and Zn. The BCR test revealed that compared with the spiked soil exchangeable proportion of Zn, Pb, Cd in the soil and the soils adjusted by ferrous sulfate obviously declined, which implied the migration for Pb, Cd and Zn of contaminated soil could be confined.This study results show that municipal LSS can be reused in the stabilization of heavy metal contaminated soils and physical and chemical properties of LLS-stabilized soil are improved for plantation.

Xu L.,Environmental Protection Research Institute of Light Industry
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011

The aim of this study was to isolate high-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs) degrading bacterial strains, and to study their degradation potential. We used sublimation method to enrich and isolate the degrading bacteria from coking plant samples. Morphological properties, the sequence homology of 16S rRNA and gyrb genes were used to identify the isolated strains. GC-MS was used to analyze the degradation potential against some HMW-PAHs. An HMW-PAHs degrading bacterium,HBS1, was obtained. HBS1 could use several HMW-PAHs such as pyrene, benzoanthracene, benzopyrene, chyrsene, indeno[ 1,2,3-cd] pyrene, benzo[g,h,i]perylene and fluoranthene as sole carbon source for growth. HBS1 was identified as Gordonia sp., based on the high sequence similarities (more than 99%) of both 16S rRNA gene and gyrb gene to those of Gordonia amicalis. When the initial concentration of pyrene was 50 mg/L, HBS1 could consume 97% of the pyrene in 17 days. One fragment of the dioxygenase gene was obtained by PCR with size about 300 bp, which was closest to the counterpart from Mycobacterium sp. with 93.8% similarity. We isolated a strain HBS1 from seriously PAHs-polluted soils and identified as a Gordonia sp. The isolate had the degradation potential of high-molecular weight polycyclic aromatic hydrocarbons.

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