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Kim D.,Stanford University | Marchetti F.,Lawrence Berkeley National Laboratory | Marchetti F.,Environmental Health Science Research Bureau | Chen Z.,Stanford University | And 19 more authors.
Scientific Reports | Year: 2013

Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry. Source


Weichenthal S.,Health Canada | Kulka R.,Health Canada | Belisle P.,McGill University | Joseph L.,McGill University | And 4 more authors.
Environmental Research | Year: 2012

Background: Few studies have examined the acute cardiorespiratory effects of specific volatile organic compound (VOC) exposures from traffic pollution. Methods: A cross-over study was conducted among 42 healthy adults during summer 2010 in Ottawa, Canada. Participants cycled for 1-h along high and low-traffic routes and VOC exposures were determined along each route. Lung function, exhaled nitric oxide, and heart rate variability were monitored before cycling and 1-4. h after the start of cycling. Bayesian hierarchical models were used to examine the relationship between 26 VOCs and acute changes in clinical outcomes adjusted for potential confounding factors. Results: Each inter-quartile range (IQR) increase in propane/butane exposure was associated with a 2.0millisecond (ms) (95% CI: 0.65, 3.2) increase in SDNN (standard deviation of normal-to-normal intervals), a 24ms 2 (95% CI: 6.6, 41) increase in HF (high frequency power), and a 65ms 2 (95% CI: 11, 118) increase in LF (low frequency power) in the hours following cycling. IQR increases in ethane and isoprene were associated with a 5.8ms (95% CI: -9.8, -1.7): decrease in SDNN and a 24ms 2 (95% CI: -44, -7.9) decrease in HF, respectively. IQR increases in benzene exposure were associated with a 1.7ppb (95% CI: 1.1, 2.3) increase in exhaled nitric oxide and each IQR increase in 3-methylhexane exposure was associated with a 102mL (95% CI: -157, -47) decrease in forced expiratory volume in 1-s. Conclusions: Exposure to traffic-related VOCs may contribute to acute changes in lung function, inflammation, or heart rate variability. © 2012 . Source


McAuliffe M.E.,Millennium: The Takeda Oncology Company | Williams P.L.,Harvard University | Korrick S.A.,Brigham and Womens Hospital | Dadd R.,Harvard University | And 3 more authors.
Human reproduction (Oxford, England) | Year: 2014

STUDY QUESTION: Is there an association between human sperm sex chromosome disomy and sperm DNA damage?SUMMARY ANSWER: An increase in human sperm XY disomy was associated with higher comet extent; however, there was no other consistent association of sex chromosome disomies with DNA damage.WHAT IS KNOWN ALREADY: There is limited published research on the association between sex chromosome disomy and sperm DNA damage and the findings are not consistent across studies.STUDY DESIGN, SIZE, AND DURATION: We conducted a cross-sectional study of 190 men (25% ever smoker, 75% never smoker) from subfertile couples presenting at the Massachusetts General Hospital Fertility Clinic from January 2000 to May 2003.PARTICIPANTS/MATERIALS, SETTING, METHODS: Multiprobe fluorescence in situ hybridization for chromosomes X, Y and 18 was used to determine XX, YY, XY and total sex chromosome disomy in sperm nuclei using an automated scoring method. The neutral comet assay was used to measure sperm DNA damage, as reflected by comet extent, percentage DNA in the comet tail, and tail distributed moment. Univariate and multiple linear regression models were constructed with sex chromosome disomy (separate models for each of the four disomic conditions) as the independent variable, and DNA damage parameters (separate models for each measure of DNA damage) as the dependent variable.MAIN RESULTS AND THE ROLE OF CHANCE: Men with current or past smoking history had significantly greater comet extent (µm: regression coefficients with 95% CI) [XX18: 15.17 (1.98, 28.36); YY18: 14.68 (1.50, 27.86); XY18: 15.41 (2.37, 28.45); Total Sex Chromosome Disomy: 15.23 (2.09, 28.38)], and tail distributed moment [XX18: 3.01 (0.30, 5.72); YY18: 2.95 (0.24, 5.67); XY18: 3.04 (0.36, 5.72); Total Sex Chromosome Disomy: 3.10 (0.31, 5.71)] than men who had never smoked. In regression models adjusted for age and smoking, there was a positive association between XY disomy and comet extent. For an increase in XY disomy from 0.56 to 1.47% (representing the 25th to 75th percentile), there was a mean increase of 5.08 µm in comet extent. No other statistically significant findings were observed.LIMITATIONS, REASONS FOR CAUTION: A potential limitation of this study is that it is cross-sectional. Cross-sectional analyses by nature do not lend themselves to inference about directionality for any observed associations; therefore we cannot determine which variable is the cause and which one is the effect. A small sample size may be a further limitation. Comparison of these findings to other studies is limited due to methodological differences.WIDER IMPLICATIONS OF THE FINDINGS: Although consistent associations across sex chromosome disomies or DNA damage measures were not observed, this study highlights the need to explore etiologies of sperm DNA damage and sex chromosome disomy to better understand the potential mechanistic overlaps between the two.STUDY FUNDING/COMPETING INTERESTS: This work was supported by NIOSH Grant T42 OH008416, and NIH/NIEHS Grants ES 009718, ES 000002, and R01 ES017457. During the study M.E.M. was affiliated with the Department of Environmental Health at the Harvard School of Public Health.N/A. © The Author 2014. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oup.com. Source


Caravaggio G.,CANMET Energy | Hebbern C.,Environmental Health Science Research Bureau | Ding L.,Environment Canada | Cakmak S.,Environmental Health Science Research Bureau
International Journal of Environmental Analytical Chemistry | Year: 2015

Thermal desorption gas chromatography mass spectrometry (TD-GC/MS) is becoming more commonly used for the quantification and identification of organic compounds in particulate matter (PM), including ambient and source PM such as diesel particulate matter (DPM). It has been proven as an alternative to the traditional solvent extraction (SE) method and liquid injection gas chromatograph mass spectrometry (LI-GC/MS). However, little information is available on how different types of TD-GC/MS systems compare to each other for analysis of real-world PM samples or to direct LI-GC/MS for analysis of PM components in a test solution. To address this, CanmetENERGY Characterization Laboratory initiated a round robin with the participation of 10 laboratories worldwide. Three sample types were analysed: (i) a test solution with a suite of pure compounds commonly found in PM, analysed by TD-GC/MS and LI-GC/MS; (ii) a DPM sample, analysed by TD-GC/MS and SE; and (iii) an ambient PM sample, analysed by TD-GC/MS. The first part of the study showed good overall performance and comparability between the different TD-GC/MS systems and LI-GC/MS method for the analysis of PM components in a test solution, with some variability of results due to system types and parameters used, concentration of calibration standards, and whether or not an internal standards was used. The analysis of the DPM sample showed greater variability between laboratories and methods as many PM components were present near the detection limit and matrix effects particularly affected the TD-GC/MS analysis of heavier n-alkanes. In the last part of the study, for the analysis of an ambient PM sample by TD-GC/MS, the analysis of variance showed good comparison between labs for polycyclic aromatic hydrocarbons (94% non-significant), but slightly lower for n-alkanes (68%) and biomarkers (57%). © 2015 Her Majesty the Queen in Right of Canada | Natural Resources Canada | Environment Canada. Source


Niu J.,Environmental Health Science Research Bureau | Rasmussen P.E.,Environmental Health Science Research Bureau | Rasmussen P.E.,University of Ottawa | Wheeler A.,Water | And 2 more authors.
Atmospheric Environment | Year: 2010

Factors and sources affecting measurement uncertainty associated with monitoring metals in airborne particulate matter (PM) were investigated as part of the Windsor, Ontario Exposure Assessment Study (WOEAS). The assessment was made using co-located duplicate samples and a comparison of two analytical approaches: ED-XRF and ICP-MS. Sampling variability was estimated using relative percent difference (RPD) of co-located duplicate samples. The comparison of ICP-MS and ED-XRF results yields very good correlations (R2 ≥ 0.7) for elements present at concentrations that pass both ICP-MS and ED-XRF detection limits (e.g. Fe, Mn, Zn, Pb and Cu). PM concentration ranges (median, sample number) of 24-h indoor PM10 and personal PM10 filters, and outdoor PM2.5 filters were determined to be 2.2-40.7 (11.0, n = 48) μg m-3, 8.0-48.3 (11.9, n = 48) μg m-3, and 17.1-42.3 (21.6, n = 18) μg m-3, respectively. The gravimetric analytical results reveal that the variations in PM mass measurements for same-day sampling are insignificant compared to temporal or spatial variations: 92%, 100% and 96% of indoor, outdoor and personal duplicate samples, respectively, pass the quality criteria (RPD ≤ 20%). Uncertainties associated with ED-XRF elemental measurements of S, Ca, Mn, Fe and Zn for 24-h filter samples are low: 78%-100% of the duplicate samples passed the quality criteria. In the case of 24-h filter samples using ICP-MS, more elements passed the quality criteria due to the lower detection limits. These were: Li, Na, K, Ca, Si, Al, V, Fe, Mn, Co, Cu, Mo, Ag, Zn, Pb, As, Mg, Sb, Sn, Sr, Th, Ti, Tl, and U. Low air concentrations of metals (near or below instrumental detection limits) and/or inadvertent introduction of metal contamination are the main causes for excluding elements based on the pass/fail criteria. Uncertainty associated with elemental measurements must be assessed on an element-by-element basis. © 2009 Elsevier Ltd. Source

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