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Mustafa M.,Environmental Biochemistry and Molecular Biology Laboratory | Banerjee B.D.,Environmental Biochemistry and Molecular Biology Laboratory | Sharma T.,Environmental Biochemistry and Molecular Biology Laboratory | Tyagi V.,Environmental Biochemistry and Molecular Biology Laboratory | And 3 more authors.
Reproductive Toxicology | Year: 2015

Elevated inflammation is a known risk factor in the pathogenesis of PTB. Despite intensive research, the etiology of idiopathic PTB is still unknown. The present study was designed to explore associations of blood concentrations of organochlorine pesticides (OCPs) with inflammatory/antioxidant gene expression, and cytokines and prostaglandin levels in PTB cases. Significantly high levels of α, β-hexachlorocyclohexane (α, β-HCH), dichlorodiphenyldichloroethane (o'p'-DDD), dichlorodiphenyldichloroethylene (p'p'-DDE), increased expression of cyclooxygenase-2 (COX-2), and decreased expression of manganese superoxide dismutase (Mn-SOD) and catalase (CAT) genes were seen in PTB cases. Also, increased protein levels of interleukin-6 (IL-6) and decreased protein levels of interleukin-4 (IL-4) and prostaglandin F2α (PGF2α) were found in maternal blood of PTB cases as compared to term controls. Elevated levels of β-HCH along with high expression of COX-2 gene or low expression of Mn-SOD or CAT genes were associated with the decrease in the period of gestation (POG). © 2015 Elsevier Inc.


PubMed | Environmental Biochemistry and Molecular Biology Laboratory and University of Delhi
Type: | Journal: Reproductive toxicology (Elmsford, N.Y.) | Year: 2015

Elevated inflammation is a known risk factor in the pathogenesis of PTB. Despite intensive research, the etiology of idiopathic PTB is still unknown. The present study was designed to explore associations of blood concentrations of organochlorine pesticides (OCPs) with inflammatory/antioxidant gene expression, and cytokines and prostaglandin levels in PTB cases. Significantly high levels of , -hexachlorocyclohexane (, -HCH), dichlorodiphenyldichloroethane (op-DDD), dichlorodiphenyldichloroethylene (pp-DDE), increased expression of cyclooxygenase-2 (COX-2), and decreased expression of manganese superoxide dismutase (Mn-SOD) and catalase (CAT) genes were seen in PTB cases. Also, increased protein levels of interleukin-6 (IL-6) and decreased protein levels of interleukin-4 (IL-4) and prostaglandin F2 (PGF2) were found in maternal blood of PTB cases as compared to term controls. Elevated levels of -HCH along with high expression of COX-2 gene or low expression of Mn-SOD or CAT genes were associated with the decrease in the period of gestation (POG).


Deshmukh P.S.,Environmental Biochemistry and Molecular Biology Laboratory | Banerjee B.D.,Environmental Biochemistry and Molecular Biology Laboratory | Abegaonkar M.P.,Indian Institute of Technology Delhi | Megha K.,Environmental Biochemistry and Molecular Biology Laboratory | And 3 more authors.
Indian Journal of Biochemistry and Biophysics | Year: 2013

Use of wireless communicating devices is increasing at an exponential rate in present time and is raising serious concerns about possible adverse effects of microwave (MW) radiation emitted from these devices on human health. The present study aimed to evaluate the effects of 900 MHz MW radiation exposure on cognitive function and oxidative stress in blood of Fischer rats. Animals were divided into two groups (6 animals/group): Group I (MW-exposed) and Group II (Sham-exposed). Animals were subjected to MW exposure (Frequency 900 MHz; specific absorption rate 8.4738 × 10-5 W/kg) in Gigahertz transverse electromagnetic cell (GTEM) for 30 days (2 h/day, 5 days/week). subsequently, cognitive function and oxidative stress parameters were examined for each group. Results showed significant impairment in cognitive function and increase in oxidative stress, as evidenced by the increase in levels of MDA (a marker of lipid peroxidation) and protein carbonyl (a marker of protein oxidation) and unaltered GSH content in blood. Thus, the study demonstrated that low level MW radiation had significant effect on cognitive function and was also capable of leading to oxidative stress.


Mustafa M.D.,Environmental Biochemistry and Molecular Biology Laboratory | Pathak R.,Environmental Biochemistry and Molecular Biology Laboratory | Ahmed T.,Environmental Biochemistry and Molecular Biology Laboratory | Ahmed R.S.,Environmental Biochemistry and Molecular Biology Laboratory | And 3 more authors.
Clinical Biochemistry | Year: 2010

Objective: Oxidative stress and related gene polymorphism may be associated with the etiology of preterm labor (PTL). The present study was designed to investigate association of GSTM1 and GSTT1 gene polymorphisms with PTL and their relationship with oxidative stress markers. Design and methods: Sixty cases of PTL and sixty three subjects of full term labor (FTL) were included in the study. Multiplex PCR was performed for GSTM1 and GSTT1 genes polymorphism and oxidative stress markers were analyzed. Result: MDA and 8-OHdG levels were increased, while GSH was decreased in PTL than FTL subjects. Frequency of GSTM1-/GSTT1-(null) was significantly higher in PTL in comparison to FTL (p=0.028, OR=3.4). Subjects with GSTM1-/GSTT1+, GSTM1+/GSTT1-, GSTM1-/GSTT1- have significant differences of oxidative stress markers as compared to GSTM1+/GSTT1+ genotype. Conclusion: GSTM1-/GSTT1-(null) genotype may be one of the associated genetic factor for the increased risk of PTL. © 2010 The Canadian Society of Clinical Chemsits.


Yadav C.S.,Environmental Biochemistry and Molecular Biology Laboratory | Kumar V.,Environmental Biochemistry and Molecular Biology Laboratory | Suke S.G.,Environmental Biochemistry and Molecular Biology Laboratory | Ahmed R.S.,Environmental Biochemistry and Molecular Biology Laboratory | And 2 more authors.
Indian Journal of Geo-Marine Sciences | Year: 2010

Propoxur (2-isopropoxyphenyl N-methylcarbamate) is widely used as an acaricide in agriculture and public health programs. Studies have shown that sub-chronic exposure to propoxur can cause oxidative stress and immuno-suppression in rats. Carbamates are also known to exhibit inhibitory effect on cholinesterase activity, which is directly related to their cholinergic effects. In the present study, the effect of Withania somnifera (Ashwagandha), a widely used herbal drug possessing anti-stress and immunomodulatory properties was studied on propoxur-induced acetylcholine esterase inhibition and impairment of cognitive function in rats. Male Wistar rats were divided into four groups. Group I was treated with olive oil and served as control. Group II was administered orally with propoxur (10 mg/kg b.wt.) in olive oil, group III received a combination of propoxur (10 mg/kg b.wt.) and W. somnifera (100 mg/kg b.wt.) suspension and group IV W. somnifera (100 mg/kg b.wt.) only. All animals were treated for 30 days. Cognitive behaviour was assessed by transfer latency using elevated plus maze. Blood and brain acetylcholine esterase (AChE) activity was also assessed. Oral administration of propoxur (10 mg/kg b.wt.) resulted in a significant reduction of brain and blood AChE activity. A significant prolongation of the acquisition as well as retention transfer latency was observed in propoxurtreated rats. Oral treatment of W. somnifera exerts protective effect and attenuates AChE inhibition and cognitive impairment caused by sub-chronic exposure to propoxur. © 2010, National Institute of Science Communication and Information Resources (NISCAIR). All right reserved.


PubMed | Environmental Biochemistry and Molecular Biology Laboratory
Type: Journal Article | Journal: Clinical biochemistry | Year: 2010

Oxidative stress and related gene polymorphism may be associated with the etiology of preterm labor (PTL). The present study was designed to investigate association of GSTM1 and GSTT1 gene polymorphisms with PTL and their relationship with oxidative stress markers.Sixty cases of PTL and sixty three subjects of full term labor (FTL) were included in the study. Multiplex PCR was performed for GSTM1 and GSTT1 genes polymorphism and oxidative stress markers were analyzed.MDA and 8-OHdG levels were increased, while GSH was decreased in PTL than FTL subjects. Frequency of GSTM1-/GSTT1-(null) was significantly higher in PTL in comparison to FTL (p=0.028, OR=3.4). Subjects with GSTM1-/GSTT1+, GSTM1+/GSTT1-, GSTM1-/GSTT1- have significant differences of oxidative stress markers as compared to GSTM1+/GSTT1+ genotype.GSTM1-/GSTT1- (null) genotype may be one of the associated genetic factor for the increased risk of PTL.

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