Environment Protection Training and Research Institute EPTRI

Gachibowli, India

Environment Protection Training and Research Institute EPTRI

Gachibowli, India
SEARCH FILTERS
Time filter
Source Type

Dharma Raju C.,Jawaharlal Nehru Technological University | Dharma Raju C.,A.P.Pharma | Srinivas J.S.,Environment Protection Training and Research Institute EPTRI
International Journal of Pharmacy and Technology | Year: 2015

A simple reversed-phase chiral HPLC method has been developed and validated for direct separation of the enantiomers of Efavirenz. Separation of the enantiomers was tested on numerous commercial chiral HPLC columns. Separation was best (resolution, RS = 3.36) on an AD-RH (Polysaccharide) stationary phase. Typical retention times of the R and S enantiomers were approximately 15 and 18 min, respectively. Mobile phase composition was systematically studied to find the optimum chromatographic conditions. The degradation of Efavirenz was studied and it was observed under base hydrolysis condition. Validation of the method under the conditions selected showed it was se-lective and precise, accurate and the detector response was also a linear function of Efavirenz Enantiomer concentration. © 2015, International Journal of Pharmacy and Technology. All rights reserved.

Loading Environment Protection Training and Research Institute EPTRI collaborators
Loading Environment Protection Training and Research Institute EPTRI collaborators