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Liu D.,Environment Protection Research Institute of Light Industry | Zhang Z.,Environment Protection Research Institute of Light Industry | Zhang Z.,Tsinghua University | Zhao K.,Environment Protection Research Institute of Light Industry | And 5 more authors.
Huagong Xuebao/CIESC Journal | Year: 2011

The effects of curvature, pitch, tube length, flow rate and GT value (the product of root-mean-square velocity gradient and detention time) of a helical secondary-flow mixer used in coagulation with polyaluminum chloride (PACl) on its performance were investigated. The helical secondary-flow mixer was prepared by twining a rubber tube round a cylinder, and kaolin suspension was used as simulated wastewater for this experiment. It was found that tube length, curvature and flow rate of the mixer had highly significant impacts on mixing effect, similarly pitch had significant impact. And the impacts of the four factors decreased as follows: tube length, flow rate, curvature and pitch. 12 m was the optimal tube length among three tube lengths of 12 m, 18 m and 24 m. As for electrically neutral coagulation, the secondary-flow mixer with a tube length of 24 m also achieved an excellent mixing effect. In addition, there existed two optimal GT values for both electrically neutral and sweep coagulation. The optimal GT values were 21000 and 35000 for the former, and 19000 and 32000 for the latter. © All Rights Reserved.


Zhang H.-B.,Beijing University of Chemical Technology | Zhang H.-B.,CAS Research Center for Eco Environmental Sciences | Lin A.-J.,Beijing University of Chemical Technology | Lin A.-J.,CAS Research Center for Eco Environmental Sciences | And 5 more authors.
Huanjing Kexue/Environmental Science | Year: 2010

Three bacterial strains J1, J2, J3 which could use pyrene as the sole carbon and energy sources were isolated from soils contaminated with polycyclic aromatic hydrocarbons (PAHs) by enrichment culture. The strains were identified as Pseudomonas aeruginosa, Flavobacterium mizutaii, Brevibacillus parabrevis. according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequence. It was observed that the three strains could use pyrene at the concentrations of 50, 100, 200, 500, 1000 mg/L and after 7 days culture the concentrations of microorganisms in the liquid medium were the highest. Under the treatment of 200 mg/L pyrene the degradation rate of pyrene by strain J1, J2, J3 was 53.04%, 65.03%, 51.02%. The three strains could grow and use pyrene at the culture medium pH 4 to pH 9 and the pH 7 was the best for the microbe growth and the degradation. Compare with the dichloromethane, N, N-dimethylformamide which could increase the solution of pyrene and enhance the degradation of pyrene was the better solvent in the pyrene degradation.


Liu Y.,CAS Institute of Microbiology | Jin J.-H.,Environment Protection Research Institute of Light Industry | Liu H.-C.,CAS Institute of Microbiology | Liu Z.-P.,CAS Institute of Microbiology
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A Gram-staining-negative, non-endospore-forming, non-flagellated, non-motile and rod-shaped bacterium, designated strain LM2-5T, was isolated from activated sludge in a sequencing batch reactor used for the treatment of triphenylmethane dye effluent. The taxonomy of strain LM2-5T was studied by phenotypic, chemotaxonomic and phylogenetic methods. Strain LM2-5T was aerobic, heterotrophic and positive for oxidase but negative for catalase activity. It grew at 16-37 °C (optimum 25 °C) and at pH 5.0-8.5 (optimum between pH 6.5 and pH 7.0). NaCl was not obligatory for growth but was tolerated at concentrations up to 2.0 % (w/v) NaCl. The novel strain formed yellow colonies on trypticase soy agar. Cells of strain LM2-5T were rods that measured 0.3-0.5 μm in width and 3.0-5.0 μm in length. The predominant respiratory quinone was Q-8. The major fatty acids were iso-C15:0 and iso-C17:1 ω9c. The genomic DNA G+C content was 66.7 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, strain LM2-5T clustered with members of the genus Dokdonella and appeared most closely related to Dokdonella koreensis DS-123T (96.4% sequence similarity), Dokdonella fugitiva A3T (96.1%), Dokdonella soli KIS28-6T (95.7%) and Dokdonella ginsengisoli Gsoil 191T (95.7%). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain LM2-5T was considered to represent a novel species of the genus Dokdonella, for which the name Dokdonella immobilis is proposed. The type strain is LM2-5T (=CGMCC 1.7659T =JCM 15763T). © 2013 IUMS Printed in Great Britain.


Liu Y.,CAS Institute of Microbiology | Jin J.-H.,Environment Protection Research Institute of Light Industry | Zhou Y.-G.,CAS Institute of Microbiology | Liu H.-C.,CAS Institute of Microbiology | Liu Z.-P.,CAS Institute of Microbiology
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

A Gram-stain-negative, heterotrophic, aerobic, non-spore-forming and non-motile bacterial strain, designated LM5T, was isolated from activated sludge from a sequencing batch reactor for the treatment of effluents contaminated by malachite green. The taxonomy of strain LM5T was studied by phenotypic and phylogenetic methods. Strain LM5T formed orange colonies on R2A and YP plates. Cells were rods, 0.4-0.6 μm in diameter and 0.8-1.2 μm in length. Growth occurred at 10-35 °C (optimum, 20-25 °C), at pH 5.5-9.5 (optimum, pH 6.5-7.5) and in the presence of 0-2% (w/v) NaCl (optimum, 0.5 %). Oxidase and catalase activities were present. Flexirubin-type pigments were present, but extracellular glycans were absent. MK-6 was the major respiratory quinone. The major fatty acids were iso-C15:0 (28.3 %) and iso-C17:1ω9c (13.8 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LM5T was a member of the genus Flavobacterium with highest sequence similarity to Flavobacterium soli DS-6T (93.2%) and Flavobacterium lindanitolerans IP-10T (92.9%). Together with F. lindanitolerans IP-10T, strain LM5T formed a distinct lineage in the phylogenetic tree. The DNA G+C content was 52±0.6 mol% (HPLC), which is significantly higher than that of other species of the genus Flavobacterium (30-41 mol%). Based on phylogenetic and phenotypic evidence, strain LM5T is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium caeni sp. nov. is proposed; the type strain is LM5T (=CGMCC 1.7031T=NBRC 104239T). © 2010 IUMS.

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