EnviroLogix Inc.

Portland, ME, United States

EnviroLogix Inc.

Portland, ME, United States
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The invention generally provides a sample preparation vessel including a flexible substrate defining at least one sealable opening adapted and configured to receive a solid sample; at least one fitting; and at least one filter adjacent to the at least one fitting, the filter adapted and configured to permit extracted fluids to exit the vessel while retaining solid particles, as well as vessels, circuits, systems, and related methods for sample preparation, extraction, and analysis.


The present invention features compositions and methods for amplifying a target oligonucleotide in a sample, including detection of the target oligonucleotide in real time.


The present invention features compositions and methods for amplifying a target oligonucleotide in a sample, including detection of the target oligonucleotide in real time.


Patent
Envirologix Inc. | Date: 2017-03-08

The present invention features compositions and methods for detecting Huanglongbing (HLB) in citrus trees and insects. In one aspect, the invention provides a method of detecting a Huanglongbing (HLB) infection in a citrus grove involving obtaining an extract from a biological sample derived from a citrus grove, contacting the extract with forward and reverse primers that specifically bind a Candidutus nucleic acid molecule in the presence of a nicking enzyme, dNTPs, and a polymerase under conditions permissive for the isothermal amplification of the nucleic acid molecule; and detecting a Candidutus amplicon in the extract.


The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction.


Patent
Envirologix Inc. | Date: 2015-04-28

The present invention features compositions and methods for detecting Huanglongbing (HLB) in citrus trees and insects. In one aspect, the invention provides a method of detecting a Huanglongbing (HLB) infection in a citrus grove involving obtaining an extract from a biological sample derived from a citrus grove, contacting the extract with forward and reverse primers that specifically bind a Candidutus nucleic acid molecule in the presence of a nicking enzyme, dNTPs, and a polymerase under conditions permissive for the isothermal amplification of the nucleic acid molecule; and detecting a Candidutus amplicon in the extract.


The present invention features compositions and methods for amplifying a target oligonucleotide in a sample comprising one or more primer oligonucleotides comprising a 5 nicking enzyme recognition site and a 3-terminal region comprising a 2-modified nucleotide. These methods are compatible with target oligonucleotides amplified using a nicking amplification reaction.


Patent
Envirologix Inc. | Date: 2016-01-15

The present invention features rapid and accurate methods for detecting Salmonella (e.g., in a food product, environmental sample, biological sample or other material).


The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction.


The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction.

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