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Portland, ME, United States

Dutta V.,North Carolina State University | Dutta V.,EnviroLogix Inc. | Elhanaf D.,North Carolina State University | Kathariou S.,North Carolina State University
Applied and Environmental Microbiology | Year: 2013

Analysis of a panel of 116 Listeria monocytogenes strains of diverse serotypes and sources (clinical, environment of food processing plants, and food) revealed that all but one of the 71 benzalkonium chloride-resistant (BCs) isolates harbored bcrABC, previously identified on a large plasmid (pLM80) of the 1998-1999 hot dog outbreak strain H7858. In contrast, bcrABC was not detected among BC-susceptible (BCs) isolates. The bcrABC sequences were highly conserved among strains of different serotypes, but variability was noted in sequences flanking bcrABC. The majority of the BCr isolates had either the pLM80-type of organization of the bcrABC region or appeared to harbor bcrABC on the chromosome, adjacent to novel. Transcription of bcrABC was induced by BC (10 μg/ml) in strains of different serotypes and diverse bcrABC region organization. These findings reveal widespread dissemination of bcrABC across BCr L. monocytogenes strains regardless of serotype and source, while also suggesting possible mechanisms of bcrABC dissemination across L. monocytogenes genomes. © 2013, American Society for Microbiology. Source


The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction.


The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction.


Trademark
EnviroLogix Inc. | Date: 2016-01-06

molecular detection kits comprised of reagents, buffers and vessels for laboratory or research use; chemical test kits for molecular detection for laboratory or research use. Molecular detection kits comprised of reagents, buffers and vessels for medical diagnostic use.


The present invention features compositions and methods for amplifying a target oligonucleotide in a sample comprising one or more primer oligonucleotides comprising a 5 nicking enzyme recognition site and a 3-terminal region comprising a 2-modified nucleotide. These methods are compatible with target oligonucleotides amplified using a nicking amplification reaction.

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