Entity

Time filter

Source Type

South Korea

Song E.J.,Chungnam National University | Song E.J.,Brain Bio | Song E.J.,Ensoltek Co. | Do Y.-J.,Chungnam National University | And 6 more authors.
Journal of Molecular Structure | Year: 2015

Abstract Receptor-interacting protein kinases 1 (RIPK1) plays an important role in necroptotic disease; it is therefore useful to identify structural characteristics controlling RIPK1 activity. Serine residues such as Ser89 and Ser161 have been reported to be important for RIPK1 activity. ATP binding to the RIPK1 hinge region occurs prior to the transfer of the phosphate group to serine residues. We therefore investigated the regulatory function of residues in the RIPK1 hinge region using a combination of molecular modeling and protein stability experiments. We analyzed the structure and evaluated the kinetic activity and stability of RIPK1 hinge region mutants. In this way, we identified Glu93 and Glu96 as key residues that regulate RIP1K activity, suggesting that mutation of these residues might be related to necroptotic diseases. The presence of a clinical mutation in RIPK1 Glu93 in endometritis patients is consistent with our data. © 2015 Elsevier B.V. Source


Kim Y.-O.,South Korean National Fisheries Research and Development Institute | Han Y.-H.,Ensoltek Co. | Moon J.Y.,South Korean National Fisheries Research and Development Institute | Kim D.-G.,South Korean National Fisheries Research and Development Institute | And 5 more authors.
Animal Cells and Systems | Year: 2014

We isolated a metalloprotease (MP) homologue from an abalone muscle cDNA library. A 3284-kb full-length cDNA encoding a predicted polypeptide of 667 amino acids was sequenced. The abalone MP Haliotis discus hannai (HdMP) exhibited a domain structure typical of the peptidase M4 family, a 21-amino acid N-terminal hydrophobic signal sequence followed by a long propeptide sequence of 347 amino acids and the mature protease domain comprising 299 amino acids. The mature region contains features characteristic of a zinc protease, including a zinc-binding motif (HEXXH) and an active site. The protein showed 32-38% amino acid sequence identity with other known MP sequences and with a hypothetical protein from owl limpet. The mRNA transcript is expressed in almost all tissues, with high expression in the mantle and adductor muscle of healthy abalones, and is expressed constitutively during the early developmental stages after fertilization. Lipopolysaccharide or poly I:C stimulation induced the expression of the HdMP transcript in the digestive track and gills of abalones. Collectively, these results suggest that HdMP could play multiple roles in defense, the immune response, growth, and regulation of reproduction. © 2014 © 2014 Korean Society for Integrative Biology. Source


Patent
Ensoltek Co. | Date: 2010-02-12

The present invention provides a peptide comprising an amino acid sequence of SEQ ID NO: 1, a variant thereof and a pharmaceutically acceptable salt thereof. A novel peptide of the present invention, a variant thereof and a pharmaceutically acceptable salt thereof are effective for treating and/or preventing degenerative disc diseases, treating body organ fibrosis, treating cancer and/or treating glomerulosclerosis, and are effective for the inhibition of TGF-beta1 signaling.


Patra J.K.,Yeungnam University | Kim E.S.,National Institute of Biological Resources | Oh K.,National Institute of Biological Resources | Kim H.-J.,Ensoltek Co. | And 2 more authors.
BMC Complementary and Alternative Medicine | Year: 2014

Background: The oral cavity is the store house of different species of microorganisms that are continuously engaged in causing diseases in the mouth. The present study was conducted to evaluate the antibacterial potential of crude extracts of the aerial parts of Phytolacca americana and its natural compounds against two oral pathogens, Porphyromonas gingivalis and Streptococcus mutans, which are primarily responsible for periodontal inflammatory diseases and dental caries, as well as a nonpathogenic Escherichia coli.Methods: Crude extract and fractions from the aerial parts of P. americana (0.008-1.8 mg/mL) were evaluated for their potential antibacterial activity against two oral disease causing microorganisms by micro-assays. The standard natural compounds present in P. americana, kaempferol, quercetin, quercetin 3-glucoside, isoqueritrin and ferulic acid, were also tested for their antibacterial activity against the pathogens at 1-8 μg/mL.Results: The crude extract was highly active against P. gingivalis (100% growth inhibition) and moderately active against S. mutans (44% growth inhibition) at 1.8 mg/mL. The chloroform and hexane fraction controlled the growth of P. gingivalis with 91% and 92% growth inhibition at a concentration of 0.2 mg/mL, respectively. Kaempferol exerted antibacterial activity against both the pathogens, whereas quercetin showed potent growth inhibition activity against only S. mutans in a concentration dependent manner.Conclusion: The crude extract, chloroform fraction, and hexane fraction of P. americana possesses active natural compounds that can inhibit the growth of oral disease causing bacteria. Thus, these extracts have the potential for use in the preparation of toothpaste and other drugs related to various oral diseases. © 2014 Patra et al.; licensee BioMed Central Ltd. Source


Patra J.K.,Yeungnam University | Kim E.S.,National Institute of Biological Resources | Oh K.,National Institute of Biological Resources | Kim H.-J.,Ensoltek Co. | And 3 more authors.
Molecules | Year: 2015

The mouth cavity hosts many types of anaerobic bacteria, including Streptococcus mutans and Porphyromonas gingivalis, which cause periodontal inflammatory diseases and dental caries. The present study was conducted to evaluate the antibacterial potential of extracts of Robinia pseudoacacia and its different fractions, as well as some of its natural compounds against oral pathogens and a nonpathogenic reference bacteria, Escherichia coli. The antibacterial activity of the crude extract and the solvent fractions (hexane, chloroform, ethyl acetate and butanol) of R. pseudoacacia were evaluated against S. mutans, P. gingivalis and E. coli DH5α by standard micro-assay procedure using conventional sterile polystyrene microplates. The results showed that the crude extract was more active against P. gingivalis (100% growth inhibition) than against S. mutans (73% growth inhibition) at 1.8 mg/mL. The chloroform and hexane fractions were active against P. gingivalis, with 91 and 97% growth inhibition, respectively, at 0.2 mg/mL. None of seven natural compounds found in R. pseudoacacia exerted an antibacterial effect on P. gingivalis; however, fisetin and myricetin at 8 μg/mL inhibited the growth of S. mutans by 81% and 86%, respectively. The crude extract of R. pseudoacacia possesses bioactive compounds that could completely control the growth of P. gingivalis. The antibiotic activities of the hexane and chloroform fractions suggest that the active compounds are hydrophobic in nature. The results indicate the effectiveness of the plant in clinical applications for the treatment of dental plaque and periodontal inflammatory diseases and its potential use as disinfectant for various surgical and orthodontic appliances. © 2015 by the authors; licensee MDPI. Source

Discover hidden collaborations