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He F.,Henan Agricultural University | Wang T.,Henan Agricultural University | Wang M.,Henan Agricultural University | Shi L.,Henan Agricultural University | And 3 more authors.
Acta Tabacaria Sinica | Year: 2013

Structure, crystalline and thermal properties of starch granules in matured tobacco leaves from Yunyan87, Zhongyan103 and Zhongyan202 were determined by environmental scanning electron microscope, X-ray diffractometer and simultaneous thermal analyzer. Results showed that most starch granules were spheroidal and long cylindrical, while others were irregular shape. The average diameter was 3 μm-4 μm. Layer structure was found on the surface of long cylindrical and irregular starch granules. The layer structure of Zhongyan103 and Zhongyan202 were more than Yunyan87. X-ray diffraction showed that relative crystallinities among three cultivars were significantly different, although they all showed B type diffraction spectrum. Thermal analysis showed that initial gelatinization temperature of starch granule was low, but peak temperature, terminal temperature and gelatinization enthalpy were high. There were no significant differences in gelatinization temperatures among cultivars. Gelatinization enthalpy decreased when relative crystallinity decreased.

Li Y.,Hubei Provincial Tobacco Research Institute | Li X.,Hubei Provincial Tobacco Research Institute | Wang L.,China Tobacco Hubei Industrial Co. | Wang H.,China Tobacco Hubei Industrial Co. | And 2 more authors.
Acta Tabacaria Sinica | Year: 2013

Harmful components in cigarette smoke from selenium-enriched tobacco leaves were measured and analyzed. These selenium-enriched tobacco leaves were either naturally grown in field or through spiking, or through pot experiment. Results indicated that: (1) Using natural selenium-enriched burley tobacco as raw material reduced B(a)P, TSNA, and NH3 delivery, while natural grown selenium-enriched flue-cured tobacco leaves reduced B(a)P, HCN and crotonic aldehyde delivery. (2) Adding organic selenium at 10 μg·cig-1 or inorganic selenium at 2 μg·cig-1 in cigarette blend reduced B(a)P, NNK, NNN, NAB, HCN and NH3 delivery. (3) Fertilizing exogenous selenium during agricultural process resulted in reduced yield of phenol, crotonic aldehyde, HCN, NH3, but increased yield of CO and NNK. In summary, adding selenium can be considered as a positive measure to reduce harmful effect of cigarette smoke.

Deng J.,Enshi Municipal Tobacco Company | Wang R.,Enshi Municipal Tobacco Company | Tan J.,Enshi Municipal Tobacco Company | Zhao X.,China Tobacco Hubei Industrial Co.
Acta Tabacaria Sinica | Year: 2013

Analysis of nutrient balance of tobacco field in Enshi was carried out. Result showed that: nitrogen was basically in equilibrium, while phosphorus and potassium were more than needed. Fertilization of Enshi tobacco-growing field should follow a principle of increasing nitrogenous fertilizer, stabilizing phosphate and potash fertilizer. Increasing topdressing ratio and the times, and cropping economic plants was suggested to improve nutrient utilization and reduce residue. Reducing chemical fertilizer input moderately can prevent soil acidification. Increasing organic fertilizer, especially manure, and applying tobacco straw bioorganic manure and green manures were proposed to realize tobacco-ecological nutrient circulation.

Hua D.,Henan Agricultural University | Zhang S.,Henan Agricultural University | Wang R.,Enshi Municipal Tobacco Company | Huo G.,Enshi Municipal Tobacco Company | Liu W.,Henan Agricultural University
Acta Tabacaria Sinica | Year: 2013

Experiments were conducted to investigate nitrogen (N) supply capacity of tobacco-planted soil in Enshi and N accumulation in different parts of tobacco plant using 15N isotope labeling technique. Results indicated that N content decreased after transplanting, with descending order of suckers > middle leaves > lower leaves > stem and root. N content in upper-middle-lower leaves were 2.850%, 2.524% and 2.322%, respectively. Tobacco sucker effected 1N accumulation in other parts of tobacco plant. Trend of N accumulation from soil and from fertilizer in different leaves differed. N content of upper leaves increased continually until late growth stage with more N from soil than from fertilizer. Lower position leaves absorb more fertilizer N than upper and middle leaves. It is concluded that tobacco-planting soil in Enshi contributed more N supply.

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