Li W.,Huazhong University of Science and Technology |
Li G.,Enshi Center Hospital |
Zhang Y.,Huazhong University of Science and Technology |
Wei S.,Huazhong University of Science and Technology |
And 5 more authors.
Experimental Cell Research | Year: 2015
Imbalance in osteogenesis and adipogenesis of bone marrow stromal cells is a crucial pathological process of osteoporosis. P2×7-deficient mice were previously shown to exhibit an osteopenic phenotype and abnormal fat distribution, leading us to hypothesize that P2×7R activation was involved in the differentiation of BMSCs. Consequently, we investigated the effect of P2×7R activation on osteogenic and adipogenic differentiation of BMSCs in vitro, and established an ovariectomized (OVX) osteoporosis model to test P2×7R activation on adipocytes formation, trabecular and cortical bone parameters in vivo. Our results showed that activation of P2×7R by BzATP resulted in increase in the gene expression of osteoblastic markers, the activity of alkaline phosphatase and bone mineralization, and decrease in the gene expression of adipogenic markers and the number of adipocytes generated by BMSCs. MicroCT analysis showed that BzATP treatment ameliorated the micro-architecture of trabecular bones in OVX mice, while cortical bone parameters were unaffected. H&E staining analysis showed that was increase in the volume of trabecular bone and number of trabecular bone, and decrease in bone marrow adipocytes in BzATP-treated OVX mice compared with OVX mice. Also, activation of P2×7R transduced to ERK1/2 and JNK signaling pathways. This transduction was prevented by BBG, U0126, and SP600125. U0126 and SP600125 prevented BzATP-induced up-regulation of osteogenic-related genes expression and down-regulation of adipogenic-related genes expression. These data suggest that BzATP activates the differentiation of BMSCs into osteoblasts but not adipocytes by stimulating ERK1/2 and JNK signaling pathways in a P2×7R-dependent way. © 2015 Elsevier Inc.
Xiang X.,Enshi Center Hospital |
Liu C.,Wuhan University |
Du Z.,Wuhan University
Medical Journal of Wuhan University | Year: 2014
Prone position ventilation is a supplementary measure of mechanical ventilation. It can improve oxygenation by recruiting dorsal lung areas, optimizing ventilation and perfusion matching, facilitating the drainage of secretions and reducing mediastinal oppression to lung tissue.Adverse effects associated with prone position ventilation are rare and greatly avoidable.Exsiting evidence confirms that early application and extended time of prone position ventilation may reduce the mortality of patient with severe acute respiratory distress syndrome(ARDS).
Chen P.-F.,Enshi Center Hospital |
Wei W.-B.,Enshi Center Hospital |
Tan Y.-Z.,Enshi Center Hospital |
Hu C.,Enshi Center Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013
Background: Hepatocyte growth factor is a key cytokine for in vitro inducing the differentiation of bone marrow mesenchymal stem cells into liver stem cells. Basic fibroblast growth factor can not only increase the proliferation rate of mesenchymal stem cells and its life, but also can maintain the multilineage differentiation potential of mesenchymal stem cells in the proliferation process. Objective: To investigate the possibility of rat bone marrow mesenchymal stem cells to differentiate into hepatocyte-like cells induced by hepatocyte growth factor and basic fibroblast growth factor in vitro. Methods: Bone marrow mesenchymal stem cells were collected from femora of Sprague-Dawley rats. The harvested bone marrow mesenchymal stem cells were separated and purified by whole bone marrow adherent culture method, and passaged in vitro. The bone marrow mesenchymal stem cells were identified by flow cytometry and osteogenic induction. The cells were divided into groups: (1) M0 group: as a negative control, treated without any factor; (2) M1 group: as the positive control group, treated with 20 μg/L hepatocyte growth factor; (3) M2 group: treated with 20 μg/L hepatocyte growth factor+5 μg/L basic fibroblast growth factor; (4) M3 group: treated with 20 μg/L hepatocyte growth factor+10 μg/L basic fibroblast growth factor; (5) M4 group: treated with 20 μg/L hepatocyte growth factor+20 μg/L basic fibroblast growth factor. The morphological changes were observed under inverted microscope. At different stages of differentiation, the album expressions of of mature hepatocyte phenotype marker and alpha fetoprotein of immature hepatocyte phenotype marker were detected by immunohistochemical staining. Results and Conclusion: The harvested bone marrow mesenchymal stem cells showed morphologic changes of hepatocyte after induction. The album was positively stained at 7 days after induction, its expression level was reduced at 14 days after induction, and then the expression changed into negative at 21 days after induction. The expression of alpha fetoprotein began to appear at 14 days after induction and then continued. No positive staining could be seen in the M0 group, and at the same time point, the positive staining rate in the M3 and M4 group was higher than that in the M2 group (P < 0.05). It indicates that hepatocyte growth factor and basic fibroblast growth factor can induce bone marrow mesenchymal stem cells to differentiate into hepatocyte-like cells, and there is synergistic effect between them.