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Zhang Y.,Shanghai JiaoTong University | Chen A.,Shanghai JiaoTong University | Song L.,Shanghai JiaoTong University | Li M.,Shanghai JiaoTong University | And 4 more authors.
International Heart Journal | Year: 2016

Vagus nerve stimulation (VNS), targeting the unbalanced autonomic nervous system, is a promising therapeutic approach for chronic heart failure (HF). Moreover, calcium cycling is an important part of cardiac excitation-contraction coupling (ECC), which also participates in the antiarrhythmic effects of VNS. We hypothesized that low-level VNS (LL-VNS) could improve cardiac function by regulation of intracellular calcium handling properties. The experimental HF model was established by ligation of the left anterior descending coronary artery (LAD). Thirty-two male Sprague-Daw-ley rats were divided into 3 groups as follows; control group (sham operated without coronary ligation, n = 10), HF-VNS group (HF rats with VNS, n = 12), and HF-SS group (HF rats with sham nerve stimulation, n = 10). After 8 weeks of treatment, LL-VNS significantly improved left ventricular ejection fraction (LVEF) and attenuated myocardial interstitial fibrosis in the HF-VNS group compared with the HF-SS group. Elevated plasma norepinephrine and dopamine, but not epinephrine, were partially reduced by LL-VNS. Additionally, LL-VNS restored the protein and mRNA levels of sarcoplasmic reticulum Ca2+ ATPase (SERCA2a), Na+-Ca2+ exchanger 1 (NCX1), and phospholamban (PLB) whereas the expression of ryanodine receptor 2 (RyR2) as well as mRNA level was unaffected. Thus, our study results suggest that the improvement of cardiac performance by LL-VNS is accompanied by the reversal of dysfunctional calcium handling properties including SERCA2a, NCX1, and PLB which may be a potential molecular mechanism of VNS for HF. © 2016, International Heart Journal Association. All rights reserved.


Liu B.,Ensense Biomedical Technologies Shanghai Co. | Chen Y.,Shanghai JiaoTong University | Chen Y.,Shanghai Jiading District Central Hospital Renji Hospital Jiading Branch | Luo Z.,Ensense Biomedical Technologies Shanghai Co. | And 3 more authors.
Journal of Biomaterials Science, Polymer Edition | Year: 2015

Polymer-based flexible electrodes are receiving much attention in medical applications due to their good wearing comfort. The current fabrication methods of such electrodes are not widely applied. In this study, polydimethylsiloxane (PDMS) and conductive additives of carbon nanotubes (CNTs) were employed to fabricate composite electrodes for electrocardiography (ECG). A three-step dispersion process consisting of ultrasonication, stirring, and in situ polymerization was developed to yield homogenous CNTs–PDMS mixtures. The CNTs–PDMS mixtures were used to fabricate CNTs–PDMS composite electrodes by replica technology. The influence of ultrasonication time and CNT concentration on polymer electrode performance was evaluated by impedance and ECG measurements. The signal amplitude of the electrodes prepared using an ultrasonication time of 12 h and CNT content of 5 wt% was comparable to that of commercial Ag/AgCl electrodes. The polymer electrodes were easily fabricated by conventional manufacturing techniques, indicating a potential advantage of reduced cost for mass production. © 2015 Taylor & Francis


Liu B.,Ensense Biomedical Technologies Shanghai Co. | Liu B.,Genix Biotek Technologies Shanghai Co. | Luo Z.,Ensense Biomedical Technologies Shanghai Co. | Luo Z.,Genix Biotek Technologies Shanghai Co. | And 6 more authors.
Sensors and Actuators, A: Physical | Year: 2016

Composite electrodes are fabricated for long-term electrocardiogram (ECG) measurements. The electrode consists of a polydimethylsiloxane base, silver nanowire layer, and adhesive layer. The adhesive layer is fabricated by loading carbon nanotubes into adhesive polydimethylsiloxane. An electrode patch consisting of three composite electrodes and an adhesive polydimethylsiloxane layer is fabricated and combined with a wireless acquisition system to obtain ECG measurements. The composite electrode patch is self-adhesive and can conform to the skin, achieving robust contact between electrode and skin and thus decreasing motion artifacts. The composite electrodes combined with the measurement system are successfully used for wireless long-term recording of ECG signals. Continuous testing for 8 days shows that the ECG signal amplitude decreases slightly after wearing for 6 days and can be largely recovered by cleaning with ethanol. The composite electrodes are flexible and exhibit good ECG performance, and therefore can be used in wearable medical monitoring systems. © 2016 Elsevier B.V.


Liu B.,Genix Biotek Technologies Shanghai Co. | Liu B.,Ensense Biomedical Technologies Shanghai Co. | Luo Z.,Genix Biotek Technologies Shanghai Co. | Luo Z.,Ensense Biomedical Technologies Shanghai Co. | And 6 more authors.
Journal of Biomaterials Science, Polymer Edition | Year: 2016

In this study, the concept of polymer electrodes integrated with a wireless electrocardiogram (ECG) system was described. Polymer electrodes for long-term ECG measurements were fabricated by loading high content of carbon nanotubes (CNTs) in polydimethylsiloxane. Silver nanoparticles (Ag NPs) were added to increase the flexibility of the polymer and the conductivity of the electrode. An ECG electrode patch was fabricated by integrating the electrodes with an adhesive polydimethylsiloxane (aPDMS) layer. Holes in the electrode filled with aPDMS can enable robust contact between the electrode and skin, reducing motion artifacts. A wireless ECG measurement system was developed and adapted to the polymer electrodes. The polymer electrodes combined with the measurement system were successfully applied in wireless, long-term recording of ECG signals. An eleven-day continuous test showed that the ECG signal did not degrade over time. The results of attach/detach tests demonstrated that the ECG signal was affected by motion artifacts after six attach/detach cycles. The electrodes produced are flexible and exhibit good ECG performance, and therefore can be used in wearable medical monitoring systems. The approach proposed in this study holds significant promise for commercial application in medical fields. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

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