Seoul, South Korea
Seoul, South Korea

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Kim H.-C.,Sejong University | Choi W.J.,Korea Institute of Science and Technology | Choi W.J.,Yonsei University | Ryu J.H.,Korea Institute of Science and Technology | And 4 more authors.
Applied Biochemistry and Biotechnology | Year: 2014

Dilution was employed as a pretreatment strategy to increase light transmittance and decrease ammonia toxicity in piggery effluent prior to the cultivation of microalgae. The dilution effect was quantitatively determined based on both the maximum specific nutrient consumption rate and the maximum growth coefficient to minimize the usage of diluent. The biomass productivity of microalgae was also evaluated to select the best species among the five different candidates examined. A 20-fold dilution of piggery wastewater resulted in decreased chromaticity (584 mg Pt-Co L−1) and total nitrogen (76 mg L−1), on which the microalgae cultivation was more effective for an algal growth compared to the other dilution factors. If the initial cell concentration of Scenedesmus quadricauda increased, the production of biomass tended to improve. Robust growth and harvesting of S. quadricauda were achieved, and the associated consistent removal of inorganic nutrients was accomplished during the semi-continuous cultivation of the best species. © 2014, Springer Science+Business Media New York.

Kim Y.-H.,Konkuk University | Park S.,Konkuk University | Kim M.H.,Konkuk University | Choi Y.-K.,Konkuk University | And 6 more authors.
Biomass and Bioenergy | Year: 2013

Lipids from Chlorella vulgaris were successfully extracted using an ionic liquid, [Bmim][MeSO4]. [Bmim][MeSO4] dissolved C. vulgaris, leaving the lipids insoluble. The undissolved lipids could easily be recovered due to the lower density of the lipid phase. Furthermore, ultrasound irradiation highly enhanced the extraction rate and yield with [Bmim][MeSO4]. The total amounts of lipid extracted from C. vulgaris by the Soxhlet method and the Bligh and Dyer's method were 21 and 29mg/g dry cell weight (DCW), respectively, whereas it was 47mg/g DCW with [Bmim][MeSO4]. Additionally, the amount of lipid extracted using [Bmim][MeSO4] was 1.6 times greater with ultrasound irradiation. The rate of extraction of lipids from C. vulgaris with [Bmim][MeSO4] was also 2.7 times greater with ultrasound irradiation. The fatty acid profiles of the lipids extracted using [Bmim][MeSO4] were very similar to those of the lipids obtained by Bligh and Dyer's method. © 2013 Elsevier Ltd.

Kim H.-C.,Sejong University | Choi W.J.,Korea Institute of Science and Technology | Maeng S.K.,Sejong University | Kim H.J.,Konkuk University | And 2 more authors.
Bioresource Technology | Year: 2014

The feasibility of using ozonation pretreatment was investigated for a better performance of post microalgae-based wastewater remediation when treating piggery effluent which was anaerobically digested and subsequently micro-filtered. Ozonation pretreatment at a dose of 1.1mg-O3mg-C-1 or higher significantly improved the transmittance of light illumination through the mixed liquor by decolorizing the piggery effluent as culture media, which resulted in increasing both the productivity of algal biomass and the associated removal of inorganic nutrients from the effluent. Ozonation also converted refractory organic constituents in the piggery effluent to a large number of biodegradable fractions via both partial-mineralization and low-molecularization. These fractions were facilely removed through biological assimilation during the mixotrophic cultivation of a microalga S. quadricauda. The results revealed that ozonation could be one of the most promising strategies for the pretreatment of highly-colored piggery effluent prior to algae-based wastewater treatment. © 2014 Elsevier Ltd.

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