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Wang F.,University of Science and Technology of China | Li C.,University of Science and Technology of China | Li F.,University of Science and Technology of China | Li F.,Lanzhou University | And 6 more authors.
Archives of Animal Nutrition | Year: 2016

The growth hormone receptor (GHR) is associated with animal growth and development. To investigate such effects on GHR gene expression, a total of 102 Hu lambs were randomly allocated to one of three groups (Group 1: starter diet from 7 d of age, weaning at 56 d of age; Group 2: starter diet from 42 d of age, weaning at 56 d of age; Group 3: starter diet from 7 d of age; weaning at 28 d of age). Six lambs from each group were sacrificed every 14 d to investigate the effects of starter feeding and weaning age on GHR mRNA expression in the liver and rumen. The results revealed that GHR mRNA expression was significantly higher in the liver and rumen (p < 0.05) than in other tissues. Early starter feeding up-regulated hepatic GHR mRNA expression on days 14, 28, 42 and 56 and ruminal GHR mRNA expression on days 28, 42, 70, and 84 (p < 0.05). Early weaning up-regulated hepatic GHR mRNA expression on days 56, 70 and 84 and ruminal GHR mRNA expression on days 42, 56, 70 and 84 (p < 0.05). Dietary and weaning regimes and age affected the hepatic and ruminal GHR mRNA expression. © 2016 Informa UK Limited, trading as Taylor & Francis Group.


Pan X.,University of Science and Technology of China | Liu S.,University of Science and Technology of China | Li F.,University of Science and Technology of China | Li F.,Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province | And 7 more authors.
Molecular Biology Reports | Year: 2014

Follicle-stimulating hormone receptor (FSHR) is a glycoprotein family member of G proteins coupled receptor super-family, and plays important roles in animal follicular development. In this study, we cloned the 5′ flanking region of ovine FSHR gene and analyzed its genomic structure. One special domain named the LRRNT (Leucine rich repeat N-terminal domain) was found in the prediction amino acid sequence of ovine FSHR. RT-qPCR showed that ovine FSHR was expressed widely in detected tissues and was significantly higher in sexual gland (testicle and ovary) than in other tissues (heart, liver, spleen, lung, kidney, rumen, duodenum, muscle, fat, hypothalamus and pituitary) (P < 0.05). In addition, synonymous mutation g.−47C > T of the FSHR gene was detected and confirmed to be significantly associated with the litter size (P < 0.01), the genotype of CC had 0.42 (P < 0.01) and 0.53 (P < 0.01) lambs more than TC and TT genotype, respectively. Our results indicated the association of ovine FSHR with the litter size in sheep and FSHR could be used as a candidate gene for improving reproductive traits in industrial sheep breeding. © 2014, Springer Science+Business Media Dordrecht.


Wang W.,University of Science and Technology of China | Liu S.,University of Science and Technology of China | Li F.,University of Science and Technology of China | Pan X.,University of Science and Technology of China | And 8 more authors.
International Journal of Molecular Sciences | Year: 2015

The Small Tailed Han sheep and Hu sheep are two prolific local sheep in China. In this study, the polymorphisms of BMPR-IB (Bone morphogenetic protein receptor IB), BMP-15 (Bone morphogenetic protein 15) and FSHR (follicle stimulating hormone receptor) were investigated to check whether they are associated with litter size in Small Tailed Han sheep and Hu sheep. Consequently, three polymorphisms, FecB mutation in BMPR-IB (c.746A>G), FecG mutation in BMP-15 (c.718C>T) and the mutation (g. 47C>T) in FSHR were found in the above two sheep breeds with a total number of 1630 individuals. The single marker association analysis showed that the three mutations were significantly associated with litter size. The ewes with genotype FecBB/FecBB and FecBB/FecB+ had 0.78 and 0.58 more lambs (p < 0.01) than those with genotype FecB+ /FecB+ , respectively. The heterozygous Han and Hu ewes with FecXG/FecX+ genotype showed 0.30 (p = 0.05) more lambs than those with the FecX+ /FecX+ genotype. For FSHR gene, the ewes with genotype CC had 0.52 (p < 0.01) and 0.75 (p < 0.01) more lambs than those with genotypes TC and TT, respectively. Combined effect analyses indicated an extremely significant interaction (p < 0.01) between the random combinations of BMPR-IB, BMP-15 and FSHR genes on litter size. In addition, the Han and Hu ewes with BB/G+/CC genotype harbor the highest litter size among ewes analyzed in current study. In conclusion, BMPR-IB, BMP-15 and FSHR polymorphisms could be used as genetic markers in multi-gene pyramiding for improving litter size in sheep husbandry. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Liu T.,University of Science and Technology of China | Li F.,University of Science and Technology of China | Wang W.,University of Science and Technology of China | Wang W.,Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province | And 10 more authors.
Animal Feed Science and Technology | Year: 2016

The objective of this study was to investigate the effects of early starter feeding on ruminal pH regulation and the expression levels of genes involved in rumen volatile fatty acid (VFA) metabolism in lambs. A total of 78 male Hu lambs with similar born body weights were selected. Six lambs were slaughtered immediately after birth, and the remaining 72 were equally divided into a group fed by starter diet 1 at day 7 or a group fed by starter diet 1 at day 42. The lambs from both groups were weaned at day 56 and transacted to starter diet 2 at day 59. Six lambs from each group were randomly slaughtered at days 14, 28, 42, 56, 70 and 84, respectively. The results revealed that the ruminal VFA concentration in the early provision group was higher (P <. 0.05) than that in late provision group, while the rumen pH was not affected (P >. 0.05) by early or late starter feeding. Treatment-age interactions (P <. 0.05) and age alone (P <. 0.05) affected the expressions of NHE2, NHE3, DRA, HMGCL and MCT1. The expression of NHE2, DRA and HMGCL in early provision group were lower (P <. 0.05) than that in late provision group. However, the expression of MCT1 in early provision group was higher (P <. 0.05) than that in the late provision group. The findings of this study show that early starter feeding in lambs can increase the ruminal VFA concentration and affect the expression levels of genes involved in VFA transport and pH regulation in the ruminal epithelium. © 2016 Elsevier B.V.


PubMed | University of Science and Technology of China and Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2015

The Small Tailed Han sheep and Hu sheep are two prolific local sheep in China. In this study, the polymorphisms of BMPR-IB (Bone morphogenetic protein receptor IB), BMP-15 (Bone morphogenetic protein 15) and FSHR (follicle stimulating hormone receptor) were investigated to check whether they are associated with litter size in Small Tailed Han sheep and Hu sheep. Consequently, three polymorphisms, FecB mutation in BMPR-IB (c.746A>G), FecG mutation in BMP-15 (c.718C>T) and the mutation (g. 47C>T) in FSHR were found in the above two sheep breeds with a total number of 1630 individuals. The single marker association analysis showed that the three mutations were significantly associated with litter size. The ewes with genotype FecBB/FecBB and FecBB/FecB+ had 0.78 and 0.58 more lambs (p < 0.01) than those with genotype FecB+/FecB+, respectively. The heterozygous Han and Hu ewes with FecXG/FecX+ genotype showed 0.30 (p = 0.05) more lambs than those with the FecX+/FecX+ genotype. For FSHR gene, the ewes with genotype CC had 0.52 (p < 0.01) and 0.75 (p < 0.01) more lambs than those with genotypes TC and TT, respectively. Combined effect analyses indicated an extremely significant interaction (p < 0.01) between the random combinations of BMPR-IB, BMP-15 and FSHR genes on litter size. In addition, the Han and Hu ewes with BB/G+/CC genotype harbor the highest litter size among ewes analyzed in current study. In conclusion, BMPR-IB, BMP-15 and FSHR polymorphisms could be used as genetic markers in multi-gene pyramiding for improving litter size in sheep husbandry.

Loading Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province collaborators
Loading Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province collaborators