Scripps Research Institute and Engineering Arts, Llc | Date: 2013-01-14
The invention provides methods and devices for preparing frozen vitrified samples for transmission electron microscopy. By reducing the volume of sample from microliter scale to picoliter scale, the requirement for blotting of excess fluid is minimized or eliminated.
Arizona Board Of Regents and Engineering Arts, Llc | Date: 2012-10-24
Biomolecule arrays on a substrate are described which contain a plurality of biomolecules, such as coding nucleic acids and/or isolated polypeptides, at a plurality of discrete, isolated, locations. The arrays can be used, for example, in high throughput genomics and proteomics for specific uses including, but not limited molecular diagnostics for early detection, diagnosis, treatment, prognosis, monitoring clinical response, and protein crystallography.
Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 130.88K | Year: 1999
Not Available This proposal describes a 6-month SBIR Phase I proposal to develop a preliminary design for a seating system that attenuates the vehicle acceleration effects of mineblasts. A vehicle and occupant motion analysis is presented to identify the characteristics of a mineblast acceleration pulse. Human tolerance to whole-body and body segment accelerations are compared to mineblast accelerations to assess their injury potential and to define the operational envelope of a mineblast energy attenuation system. Candidate energy absorber designs are described using static and dynamic analysis methods and advanced seat design tools. A static and dynamic test plan that verifies the function of the AAAV mineblast seat energy attenuation system is outlined. Preliminary designs for the seat structure, energy attenuator, occupant restraint system, and cushion system are presented along with a system weight budget and supporting static and dynamic analyses.
Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 149.80K | Year: 2014
DESCRIPTION (provided by applicant): Project Summary / Abstract Engineering Arts (EA) proposes the development of a detachable nozzle piezoelectric dispense system for use in Cryo-TEM (Cold - Transmission Electron Microscopy) sample preparation instrumentation. Cryo-TEM is an important tool for studying the structure of biological macromolecules in their native aqueous state. The challenging and laborious process of preparing 'thin-ice' frozen aqueous samples for Cryo-TEM has remained essentially unchangedfor decades. Typically a single aqueous biological sample (~3 l) is manually applied onto a thin porous Electron Microscopy (EM) grid which is then blotted to remove excess sample and then plunged into liquid-ethane for flash freezing. This process hopefully leaves enough regions on the grid with the proper vitrified 'thin-ice', typically 50 to 200nm thickness, required for EM imaging. The detachable piezoelectric nozzle dispense system proposed here is a key innovation that will lead to productivity,
Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase II | Award Amount: 1.59M | Year: 2003
DESCRIPTION (provided by applicant): The proposed research follows two distinct yet complementary paths. One path involves developing and testing novel piezoelectric pipetting, dispensing, sensing and housing technology and integrating this into a fully automated piezoelectric pipetting system. Innovative piezoelectric-based technology enables pipetting of sub-nanoliter volumes of fluid and continuous monitoring to detect clogged tips and other operational states. The other path involves doing cancer-related genomics research in collaborative studies with the Fred Hutchinson Cancer Research Center (F.H.C.R.C.) to use the proposed piezoelectric pipetting system to 1) develop more robust oligonucleotide or cDNA-based microarray platforms for gene expression analyses, 2) complete the development of a cDNA microarray-based platform for simultaneous genotyping and gene expression monitoring and 3) develop novel, high-throughput DNA microarray-based assays of cellular function that will make it possible to assay phenotypic effects of silencing specific genes on a genomic scale. On the one hand, the molecular-biology research provides a real-world application to help focus the design of the piezoelectric pipetting system and validate its performance. On the other hand, the system provides the enabling technology in terms of reliability, speed, experiment design flexibility, spot reproducibility and density to make the cancer genomics and functional genomics related research feasible. The ultimate goal of the proposed research is to have the two paths come together, resulting in a fully functional and tested general-purpose, automated, piezoelectric, fluid pipetting system with the reliability and performance to empower cancer related, genomic and functional genomics research.