Huang J.-F.,Kaohsiung Municipal Hsiao Kang Hospital |
Huang J.-F.,Graduate Institute of Medicine |
Dai C.-Y.,Kaohsiung Medical University |
Yu M.-L.,Kaohsiung Medical University |
And 10 more authors.
Hepatology Research | Year: 2011
Aim: Hepatitis C virus (HCV) proteins can activate the PI3K/Akt pathway which is involved in multiple cellular functions such as inflammatory cell activation and liver fibrosis. The aim of the present study was to elucidate the correlation between Akt expression and liver fibrosis staging in chronic hepatitis C (CHC) patients. Methods: Paraffin-embedded liver sections from 133 consecutive treatment-naïve CHC patients were recruited. The expression features of Akt were analyzed using immunohistochemical methods and the results were compared with histological, virological and biochemical profiles. Results: The 73 patients with high Akt expression carried higher histological activity index scores (6.52±2.5 vs 5.62±2.4, P=0.04) and advanced fibrosis (72.7% vs 26.3%, P<0.01) than other 60 patients with low Akt expression. The high Akt expression showed a significant incremental trend dependent on fibrosis stages, from 33.3% of F0 to 85.7% of F4 (P=0.005). Akt expression was not correlated with degrees of steatosis and virological features of HCV infection, such as viral load and genotypes. Multivariate logistic regression analysis showed advanced fibrosis was the most significant factor associated with high Akt expression (odds ratio=3.16). Conclusion: Hepatic Akt expression correlated with advanced liver fibrosis in CHC patients. © 2011 The Japan Society of Hepatology.
Camargo E.G.,Endocrine Division |
Soares A.A.,Endocrine Division |
Detanico A.B.,Endocrine Division |
Weinert L.S.,Endocrine Division |
And 3 more authors.
Diabetic Medicine | Year: 2011
Aims To analyse the performances of Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) and of Modification of Diet in Renal Disease (MDRD) study equations to estimate glomerular filtration rate (GFR) in patients with Type-2 diabetes mellitus with GFRs >60-ml/min and in healthy volunteers. Methods This cross-sectional study included 111 individuals (56 patients with Type-2 diabetes and 55 healthy volunteers), aged 58-±-9-years; 54 individuals were men (49%) and ninety-eight (88%) were white. Glomerular filtration rate was measured by the 51Cr-EDTA single-injection method (51Cr-GFR) and estimated according to the standardized MDRD and CKD-EPI equations. Serum creatinine was measured by a traceable Jaffe method. Bland-Altman analysis was used to examine the agreement between measured and estimated GFR. Bias, accuracy and precision were evaluated. Results In diabetic individuals, 51Cr-GFR was 106 ± 27-ml/min/1.73 m2, CKD-EPI-estimated GFR 82-±-18 ml/min/1.73-m2 and MDRD-estimated GFR 80 ± 21-ml/min/1.73 m2 (P-<-0.001). In healthy volunteers, the corresponding values were 98 ± 20, 89 ± 13 and 84-±-14-ml/min/1.73-m2 (P < 0.001). The accuracy of CKD-EPI (P30) was higher in healthy volunteers than in diabetic patients (90 vs. 66%, respectively, P-<-0.001). The MDRD equation performed as poorly as the CKD-EPI equation in individuals with Type-2 diabetes. Conclusions The CKD-EPI equation is less accurate in patients with Type-2 diabetes when compared with healthy individuals, with a 2.5-fold greater bias. © 2010 The Authors. Diabetic Medicine © 2010 Diabetes UK.