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Jurjevic Z.,EMSL Analytical Inc. | Peterson S.W.,National United University | Solfrizzo M.,CNR Institute of Sciences of Food Production | Peraica M.,Institute for Medical Research and Occupational Health
Mycotoxin Research | Year: 2013

Nine recently described Aspergillus species and four known species in section Versicolores were tested for their ability to produce sterigmatocystin on two liquid media, Czapek w/20 % Sucrose Broth and Yeast Extract Broth grown in the dark for 1 week at 25 C. Detection and quantification of ST were performed by reversed-phase liquid chromatography coupled with electrospray ionization ion trap mass spectrometry. Limit of detection was 3 ng/mL and limit of quantification was 10 ng/mL. Nine newly described Aspergillus species from various substrates, A. amoenus, A. creber, A. cvjetkovicii, A. fructus, A. jensenii, A. puulaauensis, A. subversicolor, A. tennesseensis and A. venenatus in section Versicolores were found to produce sterigmatocystin. Production was confirmed in recently collected isolates of A. protuberus and A. versicolor. A. austroafricanus and A. tabacinus did not produce sterigmatocystin. © 2013 Society for Mycotoxin Research and Springer-Verlag Berlin Heidelberg.


Kozak-Muiznieks N.A.,Centers for Disease Control and Prevention | Lucas C.E.,Centers for Disease Control and Prevention | Brown E.,Centers for Disease Control and Prevention | Pondo T.,Centers for Disease Control and Prevention | And 4 more authors.
Journal of Clinical Microbiology | Year: 2014

Since the establishment of sequence-based typing as the gold standard for DNA-based typing of Legionella pneumophila, the Legionella laboratory at the Centers for Disease Control and Prevention (CDC) has conducted routine sequence-based typing (SBT) analysis of all incoming L. pneumophila serogroup 1 (Lp1) isolates to identify potential links between cases and to better understand genetic diversity and clonal expansion among L. pneumophila bacteria. Retrospective genotyping of Lp1 isolates from sporadic cases and Legionnaires' disease (LD) outbreaks deposited into the CDC reference collection since 1982 has been completed. For this study, we compared the distribution of sequence types (STs) among Lp1 isolates implicated in 26 outbreaks in the United States, 571 clinical isolates from sporadic cases of LD in the United States, and 149 environmental isolates with no known association with LD. The Lp1 isolates under study had been deposited into our collection between 1982 and 2012. We identified 17 outbreak-associated STs, 153 sporadic STs, and 49 environmental STs. We observed that Lp1 STs from outbreaks and sporadic cases are more similar to each other than either group is to environmental STs. The most frequent ST for both sporadic and environmental isolates was ST1, accounting for 25% and 49% of the total number of isolates, respectively. The STs shared by both outbreak-associated and sporadic Lp1 included ST1, ST35, ST36, ST37, and ST222. The STs most commonly found in sporadic and outbreak-associated Lp1 populations may have an increased ability to cause disease and thus may require special attention when detected.Copyright © 2014, American Society for Microbiology. All Rights Reserved.


Mejia L.C.,U.S. Department of Agriculture | Mejia L.C.,Rutgers University | Mejia L.C.,Smithsonian Tropical Research Institute | Castlebury L.A.,U.S. Department of Agriculture | And 3 more authors.
Studies in Mycology | Year: 2011

Members of the genus Plagiostoma inhabit leaves, stems, twigs, and branches of woody and herbaceous plants predominantly in the temperate Northern Hemisphere. An account of all known species of Plagiostoma including Cryptodiaporthe is presented based on analyses of morphological, cultural, and DNA sequence data. Multigene phylogenetic analyses of DNA sequences from four genes (β-tubulin, ITS, rpb2, and tef1-α) revealed eight previously undescribed phylogenetic species and an association between a clade composed of 11 species of Plagiostoma and the host family Salicaceae. In this paper these eight new species of Plagiostoma are described, four species are redescribed, and four new combinations are proposed. A key to the 25 accepted species of Plagiostoma based on host, shape, and size of perithecia, perithecial arrangement in the host, and microscopic characteristics of the asci and ascospores is provided. Disposition of additional names in Cryptodiaporthe and Plagiostoma is also discussed. © 2011 by the CBS Fungal Biodiversity Centre.


Duncan K.E.,Fox Chase Cancer Center | Cook P.M.,U.S. Environmental Protection Agency | Gavett S.H.,U.S. Environmental Protection Agency | Dailey L.A.,U.S. Environmental Protection Agency | And 4 more authors.
Particle and Fibre Toxicology | Year: 2014

Background: An abnormally high incidence of lung disease has been observed in the residents of Libby, Montana, which has been attributed to occupational and environmental exposure to fibrous amphiboles originating from a nearby contaminated vermiculite mine. The composition of Libby amphibole (LA) is complex and minimal toxicity data are available. In this study, we conduct a comparative particle toxicity analysis of LA compared with standard reference asbestiform amphibole samples.Methods: Primary human airway epithelial cells (HAEC) were exposed to two different LA samples as well as standard amphibole reference samples. Analysis of the samples included a complete particle size distribution analysis, calculation of surface area by electron microscopy and by gas adsorption and quantification of surface-conjugated iron and hydroxyl radical production by the fibers. Interleukin-8 mRNA levels were quantified by qRT-PCR to measure relative pro-inflammatory response induced in HAEC in response to amphibole fiber exposure. The relative contribution of key physicochemical determinants on the observed pro-inflammatory response were also evaluated.Results: The RTI amosite reference sample contained the longest fibers and demonstrated the greatest potency at increasing IL-8 transcript levels when evaluated on an equal mass basis. The two LA samples and the UICC amosite reference sample consisted of similar particle numbers per milligram as well as similar particle size distributions and induced comparable levels of IL-8 mRNA. A strong correlation was observed between the elongated particle (aspect ratio ≥3:1) dose metrics of length and external surface area. Expression of the IL-8 data with respect to either of these metrics eliminated the differential response between the RTI amosite sample and the other samples that was observed when HAEC were exposed on an equal mass basis.Conclusions: On an equal mass basis, LA is as potent as the UICC amosite reference sample at inducing a pro-inflammatory response in HAEC but is less potent than the RTI amosite sample. The results of this study show that the particle length and particle surface area are highly correlated metrics that contribute significantly to the toxicological potential of these amphibole samples with respect to the inflammogenic response induced in airway epithelial cells. © 2014 Duncan et al.; licensee BioMed Central Ltd.


Jurjevic Z.,EMSL Analytical Inc. | Peterson S.W.,National United University
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

In sampling fungi from the built environment, two isolates that could not confidently be placed in described species were encountered. Phenotypic analysis suggested that they belonged in Aspergillus sect. Usti. In order to verify the sectional placement and to assure that they were undescribed rather than phenotypically aberrant isolates, DNA was isolated and sequenced at the beta-tubulin, calmodulin, internal transcribed spacer and RNA polymerase II loci and sequences compared with those from other species in the genus Aspergillus. At each locus, each new isolate was distant from existing species. Phylogenetic trees calculated from these data and GenBank data for species of the section Usti excluded the placement of these isolates in existing species, with statistical support. Because they were excluded from existing taxa, the distinct species Aspergillus asper (type strain NRRL 35910T) and Aspergillus collinsii (type strain NRRL 66196T) in sect. Usti are proposed to accommodate these strains. © 2016 IUMS.


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EMSL Analytical Inc. | Date: 2013-02-26

AIR SAMPLING INSTRUMENTS, DIRECT READING INSTRUMENTS, AND SAMPLE COLLECTION MEDIA FOR THE PURPOSE OF TESTING OF AIR, WATER, BULK MATERIALS, SOIL, FOOD PRODUCTS, RAW MATERIALS AND SURFACE SAMPLES FOR ASBESTOS, LEAD, SILICA, RADON, RADIOCHEMISTRY, METALS, VOLATILE ORGANIC COMPOUNDS, SEMI-VOLATILE ORGANIC COMPOUNDS, INORGANIC CHEMISTRY, GENERAL CHEMISTRY, MOLD, MILDEW, FUNGI, BACTERIA, ENDOTOXINS, MYCOTOXINS, MICROBIAL VOLATILE ORGANIC COMPOUNDS (MVOCS), ALLERGENS, PROTOZOAN PARASITES, VIRUSES, MOLD, MILDEW, FUNGI, BACTERIA, ENDOTOXINS, MYCOTOXINS, MICROBIAL VOLATILE ORGANIC COMPOUNDS (MVOCS), ALLERGENS, PROTOZOAN PARASITES, AND VIRUSES. ENVIRONMENTAL ANALYTICAL TESTING SERVICES, ANALYTICAL TESTING OF AIR, WATER, BULK MATERIALS, SOIL, FOOD PRODUCTS, RAW MATERIALS AND SURFACE SAMPLES FOR ASBESTOS, LEAD, SILICA, RADON, RADIOCHEMISTRY, METALS, VOLATILE ORGANIC COMPOUNDS, SEMI-VOLATILE ORGANIC COMPOUNDS, INORGANIC CHEMISTRY, GENERAL CHEMISTRY, MOLD, MILDEW, FUNGI, BACTERIA, ENDOTOXINS, MYCOTOXINS, MICROBIAL VOLATILE ORGANIC COMPOUNDS (mVOCS), ALLERGENS, PROTOZOAN PARASITES, AND VIRUSES; TESTING OF INDUSTRIAL HYGIENE, INDOOR AIR QUALITY, ENVIRONMENTAL CHEMISTRY, FOOD MICROBIOLOGY, WOOD MICROBIOLOGY, MATERIALS IDENTIFICATION THROUGH THE USE OF ELECTRON MICROSCOPY, OPTICAL MICROSCOPY, X-RAY DIFFRACTION, AND Q-PCR; SCIENTIFIC TESTING IN THE FIELDS OF PRODUCT FAILURE ANALYSIS, PRODUCT TESTING AND CHARACTERIZATION; FORENSIC LABORATORY SERVICES; RESEARCH AND DEVELOPMENT SERVICES IN THE FIELD OF ENVIRONMENTAL CHEMISTRY AND MICROBIOLOGY; ENVIRONMENTAL AND MATERIAL ANALYSIS; ANALYSIS OF MATERIALS THROUGH THE USE OF ELECTRON MICROSCOPY, AIR SAMPLING INSTRUMENTS, AND DIRECT READING INSTRUMENTS.


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EMSL Analytical Inc. | Date: 2010-11-23

Air sampling instruments, direct reading instruments, and sample collection media for the purposes of testing for air quality, allergens, asbestos, bacteria, chemicals, dust, endotoxins, environmental contaminants in gases, liquids or solids, fungi, hazardous waste, industrial hygiene, lead, microbiological agents, microbial volatile organic compounds (mVOCs), mildew, mold, mycotoxins, parasites, particulate matter, radon, semi-volatile organic compounds, toxins, viruses, volatile organic compounds, and yeast. Environmental analytical testing services; indoor air quality testing services; analytical testing of air, gas, liquids, solids, bulk materials, soil, food product, raw materials and surface samples for asbestos, lead, silica, radon, metals, volatile organic compounds, semi-volatile organic compounds, mold, mildew, fungi, bacteria, endotoxins, mycotoxins, microbial volatile organic compounds (mVOCs), allergens, protozoan parasites and viruses; laboratory research in the field of inorganic chemistry, general chemistry, radiochemistry, molecular biology; testing services in the field of electron microscopy, optical microscopy, x-ray diffraction, industrial hygiene, indoor air quality, environmental chemistry, food microbiology, and wood microbiology; testing services, namely, materials identification, failure analysis, product testing, characterization and forensic laboratory services, research and development services in the field of inorganic chemistry, general chemistry, radiochemistry, molecular biology, analytical testing for environmental investigations, materials testing, characterization and forensics, contamination, environmental and material analysis, and electron microscopy analysis.


Trademark
EMSL Analytical Inc. | Date: 2013-02-26

AIR SAMPLING INSTRUMENTS, DIRECT READING INSTRUMENTS, AND SAMPLE COLLECTION MEDIA FOR THE PURPOSE OF TESTING OF AIR, WATER, BULK MATERIALS, SOIL, FOOD PRODUCTS, RAW MATERIALS AND SURFACE SAMPLES FOR ASBESTOS, LEAD, SILICA, RADON, RADIOCHEMISTRY, METALS, VOLATILE ORGANIC COMPOUNDS, SEMI-VOLATILE ORGANIC COMPOUNDS, INORGANIC CHEMISTRY, GENERAL CHEMISTRY, MOLD, MILDEW, FUNGI, BACTERIA, ENDOTOXINS, MYCOTOXINS, MICROBIAL VOLATILE ORGANIC COMPOUNDS (MVOCS), ALLERGENS, PROTOZOAN PARASITES, VIRUSES, MOLD, MILDEW, FUNGI, BACTERIA, ENDOTOXINS, MYCOTOXINS, MICROBIAL VOLATILE ORGANIC COMPOUNDS (MVOCS), ALLERGENS, PROTOZOAN PARASITES, AND VIRUSES. ENVIRONMENTAL ANALYTICAL TESTING SERVICES, ANALYTICAL TESTING OF AIR, WATER, BULK MATERIALS, SOIL, FOOD PRODUCTS, RAW MATERIALS AND SURFACE SAMPLES FOR ASBESTOS, LEAD, SILICA, RADON, RADIOCHEMISTRY, METALS, VOLATILE ORGANIC COMPOUNDS, SEMI-VOLATILE ORGANIC COMPOUNDS, INORGANIC CHEMISTRY, GENERAL CHEMISTRY, MOLD, MILDEW, FUNGI, BACTERIA, ENDOTOXINS, MYCOTOXINS, MICROBIAL VOLATILE ORGANIC COMPOUNDS (mVOCS), ALLERGENS, PROTOZOAN PARASITES, AND VIRUSES; TESTING OF INDUSTRIAL HYGIENE, INDOOR AIR QUALITY, ENVIRONMENTAL CHEMISTRY, FOOD MICROBIOLOGY, WOOD MICROBIOLOGY, MATERIALS IDENTIFICATION THROUGH THE USE OF ELECTRON MICROSCOPY, OPTICAL MICROSCOPY, X-RAY DIFFRACTION, AND Q-PCR; SCIENTIFIC TESTING IN THE FIELDS OF PRODUCT FAILURE ANALYSIS, PRODUCT TESTING AND CHARACTERIZATION; FORENSIC LABORATORY SERVICES; RESEARCH AND DEVELOPMENT SERVICES IN THE FIELD OF ENVIRONMENTAL CHEMISTRY AND MICROBIOLOGY; ENVIRONMENTAL AND MATERIAL ANALYSIS; ANALYSIS OF MATERIALS THROUGH THE USE OF ELECTRON MICROSCOPY, AIR SAMPLING INSTRUMENTS, AND DIRECT READING INSTRUMENTS.


Trademark
EMSL Analytical Inc. | Date: 2011-04-11

Research reports featuring analysis, summaries, graphics, glossaries, guidelines, and resource information in the field of environmental microbiology, direct examination, and optical microscopy. Environmental microbiology analysis; environmental microbiology research services using direct examination and optical microscopy analysis; scientific information services in the field of environmental microbiology.


Trademark
EMSL Analytical Inc. | Date: 2012-12-19

RESEARCH REPORTS FEATURING ANALYSIS, SUMMARIES, GRAPHICS, GLOSSARIES, GUIDELINES AND RESOURCE INFORMATION IN THE FIELD OF ENVIRONMENTAL MICROBIOLOGY, DIRECT EXAMINATION AND OPTICAL MICROSCOPY. ENVIRONMENTAL MICROBIOLOGY ANALYSIS; ENVIRONMENTAL MICROBIOLOGY RESEARCH SERVICES USING DIRECT EXAMINATION AND OPTICAL MICROSCOPY ANALYSIS; SCIENTIFIC INFORMATION SERVICES IN THE FIELD OF ENVIRONMENTAL MICROBIOLOGY.

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