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Monteiro E.R.,State University of Maringá | Mangolin C.A.,State University of Maringá | Neves A.F.D.,State University of Maringá | Orasmo G.R.,Federal University of Piauí | And 2 more authors.
Biochemical Systematics and Ecology | Year: 2015

Microsatellite transferability was used as a method to examine the genetic diversity and structure of populations in Pilosocereus gounellei seedling samples that have potential to implement effective restoration strategies for degraded and disturbed areas of the Caatinga biome. Genomic DNA was extracted from 85 seedlings obtained from fruit collected from plants growing in native areas in the Brazilian states of Piaui (PI), Rio Grande do Norte (RN), and Bahia (BA). Six microsatellite primers were polymorphic. AMOVA showed higher genetic variation within (72%) than among (28%) the samples from the three states. The high level of similarity between the seedlings from PI, BA, and RN indicated that samples collected at any of the three sites can be used to represent the genetic diversity of the species. Seeds of plants from the three States are recommended as samples for germplasm banks and/or the production of plantlets to i) plant in areas of strategic reserves for forage, ii) deploy new cultivation areas, iii) restore degraded areas in the semi-arid Northeast, and iv) maintain ecological reserve banks and fodder with genetically divergent plants. © 2014 Elsevier Ltd.

Pereira F.E.C.B.,Federal University of Ceará | Guimaraes I.P.,Rural University | Torres S.B.,EMPARN | Benedito C.P.,UFERSA
Semina:Ciencias Agrarias | Year: 2015

This research aimed to evaluate different methods for breaking dormancy in seeds of Pithecellobium dulce (Roxb.) Benth., arboreal species of multiple use in the semiarid region of Northeast Brazil. The treatments were: control - intact seeds (T1); immersion in water until it reaches 80 °C (T2), and 100 °C (T3), concentrated sulfuric acid for five minutes (T4), 10 minutes (T5), and 15 minutes (T6), mechanical scarification with sandpaper number 80 (T7), mechanical scarification with sandpaper number 80 + immersion in water for six hours (T8), and for 12 hours (T9), soaking in water for 24 hours (T10), and for 48 hours (T11). The characteristics evaluated were such: emergency, mean emergency time, shoot length, root length, number of leaves, leaf area and total dry matter. The best method for breaking dormancy of seeds of Pithecellobium dulce (Roxb.) Benth. is mechanical scarification with sandpaper number 80.

The vigor tests are essential in programs of quality control of seeds, and the accelerated aging test is one which can be used for this purpose. In this sense, this paper aimed to study the methodology of the accelerated aging test, in order to identify different levels of vigor seed lots of coriander (Coriandrum sativum L.). To do so, four cultivars (Português, Super Verdão, Taboca and Verdão), each represented by three lots of seeds, they were subjected to the following assessments: germination, first count of germination, seedling emergence, speed of emergency seedling height, dry mass of the aerial part of the of seedlings, accelerated aging by traditional procedures and saturated NaCl (38 and 41°C for 48, 72 and 96 hours) and moisture content at the beginning of the tests and, after incubation to accelerated aging test. The statistical design was completely randomized in a factorial 3 × 2 × 3 (lot × temperature × aging period), with four replications for each cultivar and test. The accelerated aging test at 41°C/48 hours (traditional procedure) and 41°C/72 hours (saturated NaCl) was sensitive to evaluated of physiological potential of coriander seeds.

Benedito C.P.,Fitotecnia UFERSA | Ribeiro M.C.C.,UFERSA | Torres S.B.,EMPARN | Camacho R.G.V.,State University of Rio Grande do Norte | And 2 more authors.
Revista Brasileira de Sementes | Year: 2011

This study aimed to evaluate the viability of catanduva seeds stored for 210 days under controlled and uncontrolled environmental conditions (laboratory), in plastic, paper and glass packaging. The experimental design was completely randomized in a sub-subdivided plot, where the main plot were the storage conditions (laboratory and cold room) and sub-plots consisted of the packaging (plastic and paper bags and glass bottles) and sub-sub-plots of the storage times(0, 30, 60, 90, 120, 150, 180 and 210 days). The tests were conducted in the laboratory and greenhouse. Evaluations were made every 30 days of the percentage of germination and the rate of germination velocity in the laboratory and of seedling emergence in the greenhouse. The seed viability of catanduva decreased as a function of storage time during the 210 days. Catanduva seeds (Piptadenia moniliformis Benth.) can be packed for 210 days either in glass bottles or plastic bags without losing their physiological potential. The controlled environment (18-20 °C, ±60% RH) is the most suitable.

de Lima C.A.C.,EMPARN | Lima G.F.C.,EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária | Costa R.G.,Federal University of Paraiba | de Medeiros A.N.,Federal University of Paraiba | And 2 more authors.
Revista Brasileira de Zootecnia | Year: 2012

Performance, intake, and nutrients digestibility of 32 Morada Nova confined lambs were evaluated, using diets with increasing levels (0, 30, 60, 100%) of melon (Cucumis melo L.) in substitution of corn grain. Animals were about 6 months old, with an average of 15 kg of initial body weight and slaughtered at 25 kg. They were allocated in a completely randomized design with four treatments and eight replicates. The dry matter intake (DMI) presented a quadratic behavior for both sex, varying from 769 to 837 g for males, and from 722 to 646 g for females. Intakes of OM (organic matter), CP (crude protein), EE (ether extract), NDF (neutral detergent fiber), TDN (total digestible nutrients), TCH (total carbohydrate) and NFC (non fiber-carbohydrate) showed a quadratic curve, reflecting the same behavior of DMI. There was a decreasing linear response for the coefficient of apparent digestibility of dry matter (DM). The apparent digestibility coefficients of OM, CP, EE, NDF, and TCH presented an increasing linear response with the addition of melon in substitution of ground corn in the diet. Concerning the average daily gain (ADG) there was a better performance for males compared with females, as the levels of melon increased. Feed efficiency and conversion were not influenced by the treatments. There was an increasing linear effect for the days of confinement, which varied from 79 to 90. The levels of melon added to the Morada Nova lambs' diets provided reasonable performances. The treatment with 60% participation of melon shows the best economical return. On the other hand, when the level of melon is above 30%, the days in confinement increase, elevating the slaughter age. © 2012 Sociedade Brasileira de Zootecnia.

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