Embrapa Mandioca e Fruticultura CNPMF

Cruz das Almas, Brazil

Embrapa Mandioca e Fruticultura CNPMF

Cruz das Almas, Brazil

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Boechat C.L.,Federal University of Rio Grande do Sul | Accioly A.M.A.,Embrapa Mandioca e Fruticultura CNPMF
Bioscience Journal | Year: 2014

In the Northeast region of the country the physic nut emerges as a promising plant for the biodiesel production, due its high oil content in seeds. This study aimed to evaluate the behavior and seedlings quality of physic nut, depending on the dose of domestic and industrialsewage sludge. The experiments were conducted in a greenhouse with five treatments, consisting in five doses for both sewage sludge (equivalent to 0, 100, 150, 200 and 300 kg total N ha-1), in complete randomized blocks, with four replications. The variables analyzed were: shoot length, root length, leaf number, stem diameter, shoot fresh weight, root fresh weight, shoot dry weight, root dry mass and index of seedlings quality of Dickson. The industrial sewage sludge increases the variables related to the root until level of 200 kg total N ha-1 and domestic sewage sludge increases variables related to shoot at a dose of 200 kg total N ha-1, except for the dry matter of shoot and root system of physic nut seedlings. The industrial sewage sludge did not change the amount of dry matter of shoots of physic nut seedlings. The Dickson quality index (DQI) characterizes the seedlings of physic nut as good quality, however no significant difference was found between treatments.


Boechat C.L.,Federal University of Piauí | de Ribeiro M.O.,Federal University of Recôncavo da Bahia | Bomfim M.R.,Federal University of Recôncavo da Bahia | Bittencourt N.S.,Federal University of Recôncavo da Bahia | And 2 more authors.
Bioscience Journal | Year: 2015

Ever since the government began providing incentives for biofuel production, physic nut has appeared to be a promising plant, due to the high oil content of the seeds. The purpose of this study was to evaluate the nutrition of physic nut seedlings and changes in the chemical attributes of soil, based on the doses of domestic and industrial sewage sludge. Two experiments were conducted in a design with casualized blocks with four replicates using samples of a “Latossolo Amarelo” that received five doses of each sludge, equivalent to 0; 100; 150; 200 and 300 kg N total ha-1. The domestic sewage sludge raised the calcium and magnesium contents in the dry mass of the aerial part and root, and nitrogen and phosphorus in the dry mass of the aerial part of physic nut seedlings, whereas the industrial sludge raised the calcium and nitrogen in the dry mass of the aerial part and root of the physic seedlings, but did not affect the phosphorus, nitrogen, magnesium and potassium contents in the dry mass of the aerial part and root. The domestic sludge did not alter the soil pH but raised the amounts of phosphorus, calcium + magnesium, cation exchange capacity, organic matter and reduced the exchangeable aluminum, while the industrial one raised the pH and the cation exchange capacity of the soil, increased the amounts of phosphorus, calcium + magnesium, and organic matter and reduced the exchangeable aluminum present in the soil. The domestic and industrial sludges may be used to produce physic nut seedlings; however is necessary studies to evaluate the presence of heavy metals or potentially pathogenic microorganisms. © 2015, Universidade Federal de Uberlandia. All rights reserved.


Oliveira G.A.F.,Federal University of Recôncavo da Bahia | Dantas J.L.L.,Embrapa Mandioca e Fruticultura CNPMF | Oliveira E.J.,Embrapa Mandioca e Fruticultura CNPMF
Genetica | Year: 2015

The objective of this study was to evaluate information on minisatellite and microsatellite markers in papaya (Carica papaya L.). Forty minisatellites and 91 microsatellites were used for genotyping 24 papaya accessions. Estimates of genetic diversity, genetic linkage and analyses of population structure were compared. A lower average number of alleles per locus was observed in minisatellites (3.10) compared with microsatellites (3.57), although the minisatellites showed rarer alleles (18.54 %) compared with microsatellite (13.85 %). Greater expected (He = 0.52) and observed (Ho = 0.16) heterozygosity was observed in the microsatellites compared with minisatellites (He = 0.42 and Ho = 0.11), possibly due to the high number of hermaphroditic accessions, resulting in high rates of self-fertilization. The polymorphic information content and Shannon–Wiener diversity were also higher for microsatellites (from 0.47 to 1.10, respectively) compared with minisatellite (0.38 and 0.85, respectively). The probability of paternity exclusion was high for both markers (>0.999), and the combined probability of identity was from 1.65−13 to 4.33−38 for mini- and micro-satellites, respectively, which indicates that both types of markers are ideal for genetic analysis. The Bayesian analysis indicated the formation of two groups (K = 2) for both markers, although the minisatellites indicated a substructure (K = 4). A greater number of accessions with a low probability of assignment to specific groups were observed for microsatellites. Collectively, the results indicated higher informativeness of microsatellites. However, the lower informative power of minisatellites may be offset by the use of larger number of loci. Furthermore, minisatellites are subject to less error in genotyping because there is greater power to detect genotyping systems when larger motifs are used. © 2015, Springer International Publishing Switzerland.


Carmo C.D.,Federal University of Recôncavo da Bahia | Santos D.B.,Federal University of Recôncavo da Bahia | Alves L.B.,Federal University of Recôncavo da Bahia | Oliveira G.A.F.,Federal University of Recôncavo da Bahia | Oliveira E.J.,Embrapa Mandioca e Fruticultura CNPMF
Plant Molecular Biology Reporter | Year: 2015

Cassava (Manihot esculenta Crantz) lacks molecular studies for use in breeding and germplasm bank maintenance. This work aimed to develop and validate TRAP (target region amplification polymorphism) markers for cassava and evaluate their potential for structuring the genetic diversity of this species. Preliminary analyses with 396 combinations found 64 % of combinations with a good amplification pattern and polymorphism. The 69 most polymorphic TRAP combinations were used to characterize 46 cassava genotypes, from which 606 alleles (range 3 to 18 with a mean of 8.8 alleles per combination) were identified. The polymorphic information content (PIC) ranged from 0.03 to 0.38 (average 0.23), while 31 combinations showed a PIC >0.25. The resolving power (Rp) parameter ranged from 0.10 to 6.30 (average 3.21). The primers that were related to starch and carotenoid biosynthesis, cyanogenic compounds, post-harvest physiological deterioration, root formation, and defense responses were the most polymorphic (>70 % of polymorphic fragments, PIC > 0.25, and Rp > 3.21). A total of 37 private alleles were identified in 20 accessions. Bayesian clustering as implemented in STRUCTURE revealed the presence of two major clusters (K = 2) and four subclusters (K = 4). The group differentiation based on the molecular variance analysis (AMOVA) showed that most of the genetic variation is within groups but with significant differences between groups. Therefore, TRAP primers have a high polymorphism for use as a molecular tool in cassava, in addition to the association with genetic regions that may increase the chances of obtaining functional markers. © 2015 Springer Science+Business Media New York

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