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Ferrarezi A.,Sao Paulo State University | Minim V.P.,Federal University of Vicosa | Santos K.M.D.,Embrapa Goats and Sheep | Monteiro M.,Sao Paulo State University
Nutrition and Food Science | Year: 2013

Purpose: The purpose of this paper was to evaluate the impact of some labeling aspects on the consumer intent to purchase ready to drink orange juice and nectar. Design/methodology/approach: The influence of label information on the consumer intent to purchase was evaluated by conjoint analysis using a convenience sample (n=149). A factorial design with four characteristics, price, brand, information about the product and kind of beverage, was used. Three levels were established for brand and product information, and two for price and kind of beverage. Findings: Low price, product information and market leading brand had positive impact. "No preservatives/natural" was the information that most influenced consumer's purchase intent. The ideal label showed the leading brand, low price and information "no preservatives/natural". These results could be useful for strategic planning of consumer instruction and have important implications for Brazilian orange juice manufactures. Originality/value: Although the most widely consumed beverages in Brazil are ready to drink orange juice and nectar, it was unexpected that consumers did not know the differences between them and that kind of beverage was not an important factor for the purchase decision. © Emerald Group Publishing Limited.

Shiotsuki L.,Embrapa Goats and Sheep | Cardoso F.F.,Embrapa Southern Region Animal Husbandry | Silva II J.A.V.,Sao Paulo State University | Albuquerque L.G.,Sao Paulo State University
Journal of Animal Science | Year: 2013

The aim of the present study was to compare a model assuming unknown paternity and a model using genetic grouping to indicate the most adequate statistical procedure for the estimation of breeding values for animals with uncertain paternity. After data consistency, 62,212 Nellore animals, offspring of 581 bulls and 27,743 cows, were used in the analyses. The pedigree file contained 75,088 animals, including 22,810 (30.18%) offspring of multiple sires and 12,876 animals belonging to the base population with unknown parents. Three different approaches were adopted to deal with uncertain paternity of multiple-sire (MS) offspring. In the model of unknown paternity, the MS groups were ignored, and the sires of MS offspring were considered to be unknown and to belong to a single base population. In the genetic group approach, 2 definitions were used. In the first definition (GGa), "phantom parents" for animals with uncertain paternity were attributed, defining the genetic group as the MS group. In the other approach, GGb, phantom parents for animals with uncertain paternity were also attributed; however, MS offspring were clustered in genetic groups according to their year of birth, every 3 yr, on the basis of the average of male generation interval. Univariate analyses were performed under the Bayesian approach via Markov chain Monte Carlo methods. Models were compared by deviance information criteria and the conditional predictive ordinate. According to the choice criteria results, the genetic group model defined by the generation interval of males was more appropriate for predicting the genetic merit of animals with uncertain paternity. Therefore, the use of this model is recommended for the prediction of genetic merit and classification of offspring of multiple sires. © 2013 American Society of Animal Science. All rights reserved.

Olivares C.C.S.,Federal University of Fluminense | da Fonseca J.F.,Embrapa Goats and Sheep | de Almeida Camargo L.S.,Embrapa Dairy Cattle Research Center | de Souza-Fabjan J.M.G.,Federal University of Fluminense | And 2 more authors.
Small Ruminant Research | Year: 2015

This study evaluated different methods of frozen-thawed sperm selection and capacitation in goats for further use in reproductive biotechnologies. In Experiment 1, semen was processed by the following techniques: mini-Percoll, swim-up, or washing by centrifugation. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation induction by incubation with: 50. μg/mL heparin, 10. μM, 50. μM, or 100. μM of sodium nitroprusside (SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated after thawing and after each treatment of sperm selection or capacitation. In Experiment 1, washing by centrifugation presented greater (43%; P< 0.05) spermatozoa recovery rate than the other treatments. The swim-up technique showed the lowest (P< 0.05) progressive motility (41%). Spermatozoa presenting both intact PM (P=0.0002) and acrosome (P=0.0004) showed an interaction effect between the buck and swim-up technique. In Experiment 2, the addition of 100. μM SNP resulted in greater (P< 0.05) motility and vigor (38%; 4.7), respectively, than did heparin (28%; 4.3). An enhancement (P< 0.05) in vigor was obtained after all treatments in comparison with the evaluation after thawing (3.3). In conclusion, mini-Percoll was better than swim-up for preparing frozen-thawed goat sperm, whereas washing by centrifugation technique presented similar rates to mini-Percoll and could also be used. The use of 100. μM SNP resulted in better motility and vigor than heparin treatment. © 2015 Elsevier B.V.

Penna B.,Federal University of Fluminense | Libonati H.,Federal University of Fluminense | Director A.,Federal University of Fluminense | Sarzedas A.C.,Federal University of Fluminense | And 4 more authors.
Animal Reproduction Science | Year: 2013

The objective was to characterize vaginal bacteria, their antimicrobial sensitivity, and the incidence of vaginitis, in goats before and after insertion of intravaginal sponges containing progesterone. Sponges were inserted in 37 Saanen goats and removed after 6, 9 or 12. d (G6, G9 and G12). At sponge removal, all goats had clinical signs of vaginitis. Sampling was conducted just before sponge insertion and at 0, 24, 48, and 72. h after sponge removal. Vaginal secretions were subjected to standard bacteriological procedures, including isolation of bacteria, subculture, and determination of sensitivity to antimicrobials (gentamicin, cefalotin, tetracycline, ciprofloxacin, trimethoprim-sulfamethoxazole, amoxicillin and clavulanic acid, penicillin G and cefoxitin). Ciprofloxacin, gentamicin, tetracycline and trimethoprim-sulfamethoxazole were the most effective for coliforms (100% sensitivity), whereas ciprofloxacin, gentamicin and tetracycline were the most effective for cocci (100, 98.6 and 97.2% sensitivity, respectively). In contrast, the least effective antimicrobials were cefalotin for the coliforms, and penicillin for the cocci (37.5 and 64.4% sensitivity, respectively), regardless of duration of implant presence and interval from implant removal to sampling. In conclusion, insertion of intravaginal progestin-impregnated sponges induced clinical vaginitis in goats. Members of Staphylococcus genus were the most frequently recovered species of the vaginal samples cultured, and all isolates were resistant to several antimicrobials. © 2013 Elsevier B.V.

Alessandra F.,Sao Paulo State University | Karina Olbrich D.S.,Embrapa Goats and Sheep | Magali M.,Sao Paulo State University
International Journal of Food Science and Technology | Year: 2013

The aim of this study was to evaluate consumers' use and interpretation of ready to drink orange juice and nectar label information and its influence on the purchase decision. One hundred and sixty-seven consumers of ready to drink orange juice and nectar were interviewed. The labels were analysed to evaluate their conformance to Brazilian legislation. The manufacturing and shelf life date were the information most often checked, followed by health related issues. Brand, price and flavour were the most important factors for purchase decision. Brand and flavour showed significant association with consumer age. For most interviewed, 'nectar', 'whole' and 'natural' or '100% natural' were not well understood; they were not in accordance with the Brazilian legislation. 'Nectar', 'whole' and 'natural' or '100% natural' received a positive interpretation, whereas 'reconstituted juice' was considered a negative expression. Nevertheless, none of the labels completely conformed to the specific nutritional labelling legislation. © 2013 Institute of Food Science and Technology.

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