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Pereira I.S.,Federal University of Pelotas | Fachinello J.C.,Federal University of Pelotas | Antunes L.E.C.,Embrapa Clima Temperado CPACT | Errea P.,CSIC - Centro de Investigación y Tecnología Agroalimentaria | And 2 more authors.
Acta Horticulturae | Year: 2013

Graft incompatibility is a significant problem for development of new Prunus rootstocks. Recently, several authors have reported that phenylpropanoid metabolism is related with this phenomenon. The aim of the present work was to analyze the gene expression patterns of the 4-Coumarate:CoA ligase genes in compatible and incompatible graft combinations two years after grafting. The relationship of Prunus 4CLs with other plants was also studied. To perform this study different graft combinations were evaluated: the peach cultivar 'Chimarrita' (P. persica L. Batsch.) grafted onto three different rootstocks 'Capdeboscq' (P. persica L. Batsch.), 'Tsukuba 1' (P. persica L. Batsch.) and 'Umezeiro' (P. mume Sieb. et Zucc.). Transcript abundance was analyzed by real-time PCR using genespecific primers designed to amplify the three 4CL genes identified in the assembled peach genome. The results showed that the three 4CL transcripts are expressed in all combinations examined, although the quantitative levels of 4CL mRNA were higher in 'Umezeiro', the incompatible combination. The 4CL3 gene was the most highly expressed in the incompatible combination and the most divergent isoform in the phylogenetic analysis.

Fischer D.L.,Federal University of Pelotas | Vignolo G.K.,Federal University of Pelotas | Aldrighi M.G.,Federal University of Pelotas | Fachinello J.C.,Federal University of Pelotas | Antunes L.E.C.,Embrapa Clima Temperado CPACT
Acta Horticulturae | Year: 2012

Difficulty in propagation using cuttings is a factor that limits blueberry expansion. The success of propagation by cuttings depends on the capacity of adventitious root formation of the cultivar and new root system quality. This work aimed to evaluate the effect of branch type on the rooting of hardwood cuttings of the blueberry cultivars 'Delite', 'Bluebelle' and 'Briteblue'. The plant material used was derived from whip segments originating from the crown and lateral shoots from old canes, with about 7.0 cm length and 5.5 mm diameter, and 6.0 cm and 3.5 mm, respectively. The cultivar 'Briteblue' showed the highest percentage of rooting (60%), while the cultivar 'Bluebelle' had the lowest (35%). The source of cutting wood affected the percentage of cuttings that survived with 81 and 65% of the cuttings from lateral and whip canes rooting, respectively. Therefore, it was concluded that the cultivar 'Briteblue' was easy to propagate with hardwood cuttings and lateral branches provided a high percentage of rooting, independent of the cultivar.

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