Ellead Co.

Gyeonggi Do, South Korea

Ellead Co.

Gyeonggi Do, South Korea
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Kim B.,Ellead Co. | Kim J.E.,Enprani Co. | Choi B.-K.,SN Biotech Co. | Kim H.-S.,Jungwon University
Biomolecules and Therapeutics | Year: 2015

Water chestnut (Trapa japonica Flerov.) is an annual aquatic plant. In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular H2O2-induced accumulation of reactive oxygen species. In addition, water chestnut extract (WCE) inhibited lipopolysaccharide (LPS)-induced nitric oxide production and suppressed mRNA and protein expression of the inducible nitric oxide synthase gene. The cytokine array results showed that WCE inhibited inflammatory cytokine secretion. Also, WCE reduced tumor necrosis factor-α- and interleukin-6-induced nuclear factor-κB activity. Furthermore, during sodium lauryl sulfate (SLS)-induced irritation of human skin, WCE reduced SLS-induced skin erythema and improved barrier regeneration. These results indicate that WCE may be a promising topical anti-inflammatory agent. © 2015 The Korean Society of Applied Pharmacology.


Lim J.Y.,Catholic University of Korea | Oh J.H.,Catholic University of Korea | Jung J.R.,Ellead Co. | Kim S.M.,Catholic University of Korea | And 3 more authors.
Journal of Neuro-Oncology | Year: 2010

Mounting evidence suggests that lipoxygenase (LO)-catalyzed products may play a key role in the development and progression of human cancers. In this study, we analyzed the effects of a 5-LO inhibitor, which inhibits the conversion of arachidonic acid to leukotrienes, on cell proliferation and apoptosis in human malignant glioma cells, including 5-LO-expressing cells U-87MG, A172 and 5-LO non-expressing cell U373. Growth of U-87MG and A172 cells, but not that of U373 cells, was inhibited in a dose-dependent manner by treatment with MK886. Similarly, specific 5-LO silencing by small interfering RNA reduced the growth of U-87MG and A172 cells. MK886 treatment reduced 5-LO activity independently of 5-LO-activating protein (FLAP) in human malignant glioma cells. MK886 treatment also induced cell apoptosis, measured by DNA fragmentation and nuclear condensation, in U-87MG and A172 cells but there were no signs in U373 cells. Moreover, this treatment reduced ERKs phosphorylation and anti-apoptotic molecule Bcl-2 expression, and increased Bax expression in U-87MG and A172 cells. In summary, our results show there is a link between the 5-LO expression status and the extent of MK886-inhibited cell proliferation and apoptosis. Taken together, this study suggest that 5-LO is a possible target for treating patients with gliomas, and 5-LO inhibition might be potent therapy for patients with 5-LO-expressing malignant gliomas. © Springer Science+Business Media, LLC. 2009.


Joo C.G.,Morechem Co. | Lee K.H.,Morechem Co. | Park C.,Morechem Co. | Joo I.W.,Catholic Kwandong University | And 2 more authors.
Journal of Industrial and Engineering Chemistry | Year: 2012

Antioxidants are indispensable for protecting skin cell membranes against oxidative injury caused by reactive oxygen species and other free radicals. Likewise, phenolic compounds are also important barriers to such oxidative injury. In this study, we examined the correlation between increased antioxidation and the contents of phenolic compounds and amino acids in Camellia sinensis leaf extracts obtained by ultra-high pressure extraction. Compared with extracts obtained by leaching and ultrasonic extraction, to evaluate whether this method can improve the pharmacologic effects of C. sinensis leaves. The ultra-high pressure (50 and 100. MPa) extracts had tremendous enhancement of their extraction yield, 1,1-diphenyl-2-picrylhydrazyl (free radical)-scavenging activity and superoxide anion radical scavenging activity, as well as the total phenolic, amino acid, and caffeine contents. The antioxidative effects were promoted not only by the phenolic compounds contents but also by other factors such as the amino acids content in the C. sinensis leaf extracts. These effects showed pressure-dependent increase with the enhanced phenolic compound and amino acid contents of the ultra high pressure extracts. Taken together, ultra high pressure extraction is a potentially useful method for the pharmaceutical, cosmetic, and food industries, because it improves the antioxidative effects of C. sinensis leaves. © 2011 The Korean Society of Industrial and Engineering Chemistry.


Joo C.G.,Morechem Co. | Lee K.H.,Morechem Co. | Park C.,Morechem Co. | Lee B.C.,Ellead Co.
Journal of Industrial and Engineering Chemistry | Year: 2011

In this study, we investigated enhanced antioxidative activities of Pinus densiflora (. P. densiflora) root by ultra high pressure (UHP) extraction and composition analysis. Compared with extracts obtained by leaching and ultrasonic extraction, the UHP extracts had enormous enhancement of their extraction yield, free radical (1,1-diphenyl-2-picrylhydrazyl) and superoxide anion radical scavenging activities and total phenolic content and these UHP extracts showed pressure and time-dependent increase of activities and content. Composition analysis by HPLC, main component of P. densiflora root extracts was taxifolin-3-glucoside, and each extract content (mg/g) was in the order: UHP 100. MPa 3. h extract (142.4 ± 1.3) > UHP 100. MPa 1. h extract (84.7 ± 2.1) > UHP 50. MPa 1. h extract (69.7 ± 1.8) > ultrasonic 1. h extract (48.6 ± 2.5) > leaching 1. h extract (42.5 ± 1.1). When UHP extraction, the taxifolin-3-glucoside content was increased and also these results revealed pressure and time-dependent increase. Taken together, UHP extraction is a potentially useful method for the pharmaceutical, cosmeceutical, functional food industries, because it improves the antioxidative effects of P. densiflora root. © 2011 The Korean Society of Industrial and Engineering Chemistry.


Haris H.H.,Biotropics Malaysia Berhad | Ming Y.K.,Biotropics Malaysia Berhad | Moon T.K.,Ellead Co. | Kim N.S.,Ellead Co. | And 3 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

Acne is a common disease of the pilosebaceous units of the skin and topical therapy is recommended for the management of acne with comedolytic, anti-inflammatory agents, along with antimicrobials. However, topical application of these drugs leads to frequent adverse effects and also, there is an emergence of antibiotic resistance by Propionibacterium acnes. Furthermore, systemic antimicrobial usage has been causally associated with various adverse events. AVCO Acne Gel is a herbal formulation containing, Activated Virgin Coconut Oil derived from catalytic activity of lipase on virgin coconut oil (Malaysian Patent MY-140578-A). The present study was planned to evaluate the efficacy and safety of AVCO Acne Gel in management of acne vulgaris. This study was an open, single centric, non-comparative clinical trial conducted at the Ellead skin research centre, Ellead Co., Ltd. 272-1, Sehyneo-dong, Boondang-gu, Seongnam-si, Gyeonggi-do, Korea from July2, 2010 to October 6, 2010. Twenty one patients consisting of 9 males and 12 females, aged 15 to 38 were included in the study. Children below 18 years of age, patients already on medication or therapy, patients with preexisting severe skin diseases or cancer, patients prone to irritants and allergic, patients exposed to UV rays or laser and on treatment with AHA or salicylic acid, patient with stressful lifestyle and those who refused to give informed consent were excluded from the study. Pregnant and lactating women were also excluded from the study. A baseline history was obtained in order to determine the patient's eligibility for enrolment in the trial. Thereafter all patients underwent a clinical examination and thorough skin examination was done. All patients were advised to apply AVCO Acne Gel topically over the lesions, once in a day for a period of 8 weeks. All patients were followed up every two weeks and during each follow-up visit, local skin examination (dermatological assessment), instrumental assessment (sebumeter and digital photography) and subject self assessment (questionnaire) was done. The predefined primary outcome measures were acne grading, reduction of papule, pustule count and skin sebum with subject self assessment of satisfaction, Improvement and sensory evaluation. The predefined secondary outcome measures were incidence of adverse events and compliance to the treatment. Statistical analysis was done according to intention-to-treat principles. This study observed significant reduction in the acne grading (1.50 to 0.77; p<0.001), papule count (12.52 to 5.24; p<0.001), pustule count (5.38 to 1.33; p<0.001), skin sebum (107.07 to 84.21; p<0.001) after 8 weeks of application. In addition, there was a better subject self satisfaction and improvement. The overall response to the treatment also recorded a significant improvement from the second week onwards. There were no clinically significant short and long-term adverse reactions (either reported or observed), during the entire period of study and excellent patient compliance to AVCO Acne Gel was also observed. Based on these observations, it may be concluded that the AVCO Acne Gel has anti-acne activity. It is also clinically effective and safe for external usage in acne vulgaris.


Chrysanthemum zawadskii extract (CZE) was investigated to determine its effects on the transactivation activity of peroxisome proliferator-activated receptors (PPAR)-responsive element (PPRE) and activity of anti-inflammatory for improvement of skin barrier function. The treatment with CZE resulted in a significant increase in the transactivation activity of PPRE such as PPAR-α and suppression in the TNF-α-, IL-6-induced NF-κB luciferase activity and NO production. In addition, CZE promotes the expression of protein related to cornified envelope (CE) formation such as involucrin. Therefore, these results indicate that CZE can restore skin barrier homeostasis and is suggested to be an appropriate skin therapeutic agent. © 2014, Korean Institute of Chemical Engineers, Seoul, Korea.


Atopic dermatitis,which is related to dermatologic disorders and is associatedwith skin barrier dysfunction, represents an epidemic problem demanding effective therapeutic strategies. In the present study, we showed that the treatment with Eruca sativa extract resulted in a significant increase in the transactivation activity of peroxisome proliferator-activated receptor (PPAR) response element such as PPAR-α and suppression in the expression of inflammatory cytokine and antimicrobial peptides. In addition, E. sativa extract promotes the expression of filaggrin related to skin barrier protection. Quercetin and isorhamnetin, flavonoids' constituents of E. sativa, also promoted PPAR-α activity. These results indicate that E. sativa extract may be an appropriate material for improving skin barrier function as a skin therapeutic agent for atopic dermatitis. Copyright © 2014 John Wiley & Sons, Ltd.


PubMed | Yonsei University, DERMAPRO LTD. and Ellead Co.
Type: Journal Article | Journal: International journal of cosmetic science | Year: 2016

Although an ascorbic acid-loaded dissolving microneedle patch has been developed to improve anti-wrinkle effects, an efficacy evaluation with a control group has not yet been performed. In this study, the anti-wrinkle effect of an ascorbic acid-loaded dissolving microneedle patch was evaluated in a double-blind clinical study with a control group. In addition, a cumulative skin irritation and sensitization potential of the ascorbic acid-loaded dissolving microneedle patch was performed.Twenty-three subjects were selected for anti-wrinkle effect evaluation in a double-blind clinical study. Subjects were divided into two groups. Group I subjects applied an ascorbic acid-loaded dissolving microneedle patch on a crows feet area on the left side of the face and a control sample on a crows feet area on the right side of the face every 4 days. Group II subjects placed the same patches on opposite sides of the face. Global Photodamage Score and skin replica analysis were conducted by visual inspection and skin visiometer, respectively. A skin irritation and sensitization assessment was performed on 51 subjects using the modified Shelanski & Shelanski procedure. Cumulative skin irritation potential and skin sensitization of the ascorbic acid-loaded dissolving microneedle patch and control sample were evaluated.Skin treated with the ascorbic acid-loaded dissolving microneedle patch showed a statistically significant improvement in both the Global Photodamage Score and visiometer R values (P < 0.05) compared with the control sample. The R2 value (maximum roughness), in particular, showed a highly significant improvement (P < 0.01). The skin irritation and sensitization assessment demonstrated that the ascorbic acid-loaded dissolving microneedle patch did not induce any cumulative skin irritation potential or skin sensitization.An ascorbic acid-loaded dissolving microneedle patch produced a significant anti-wrinkle effect without skin irritation and sensitization problems. This cosmetic dissolving microneedle patch can be used efficiently in the anti-wrinkle cosmetic field with patient convenience.


PubMed | Korea Advanced Institute of Science and Technology, Kyung Hee University and Ellead Co.
Type: | Journal: European journal of pharmacology | Year: 2015

We investigated the inhibitory effects of a niacinamide derivative, N-Nicotinoyl dopamine (NND) on melanogenesis. NND inhibits melanosome transfer in a normal human melanocyte-keratinocyte co-culture system and through phagocytic ability without affecting viability of cells while it did not show inhibitory effects of tyrosinase and melanin synthesis in B16F10 mouse melanoma cells. In addition, safety of NND was verified through performing neural stem cell morphology assay. Our findings indicate that NND may potentially be used for cosmetic industry for improvement of skin whitening and therapies related with several skin disorders, and the effect of NND may be acquired via reduction of melanosome transfer.


PubMed | Ellead Co.
Type: Journal Article | Journal: International journal of cosmetic science | Year: 2015

The physiological characteristics of the skin are varied greatly, depending on gender, age, region and race, and many dermatologic researches have been performed through various research methods. This study aimed to examine how Korean mens skin conditions were influenced by temperature or humidity changes caused by seasonal rotations.A total of 100 healthy Korean men, age range 20-59years, participated in the study for both summer and winter. We compared on the characteristics of skin between summer and winter. The skin hydration, skin pH and TEWL were evaluated on the forehead, cheek and forearm. The skin sebum content of the glabella, nasal ala and cheek was measured using Sebumeter() (SM810, Courage+Khazaka, Germany). Cutometer() (MPA 580 Courage+Khazaka, Germany) the elasticity was measured by on the cheeks, and PRIMOS lite() (Phase shift Rapid in vivo Measurement of Skin, GFMesstechnik GmbH, Germany) was used to evaluate wrinkles on crows feet. Lastly, in addition, the skin pore of the face was measured using the Janus() (PSI, Korea) which is a facial analysis system.The results were as follows: the comparison of hydration in summer and winter shows significant differences in their forehead, cheeks and forearm. The pH values of the skin surface were generally higher in winter, and significantly different on each site, and the sebum content was higher in summer than in winter. As a result of the pore measurement, the summer showed more pores compared to the winter, and there was a statistically significant difference in skin pores between summer and winter. The sensitivity measured by stinging test increases significantly more in winter than in summer. However, there were no seasonal differences in wrinkles and skin brightness.The skin surface pH, TEWL, sebum content, hydration, elasticity, wrinkles, skin pore and skin sensitivity vary with seasons and body regions in Korean men.

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