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Pohang, South Korea

Joo C.G.,Morechem Corporation | Lee K.H.,Morechem Corporation | Park C.,Morechem Corporation | Lee B.C.,Ellead Co.
Journal of Industrial and Engineering Chemistry | Year: 2011

In this study, we investigated enhanced antioxidative activities of Pinus densiflora (. P. densiflora) root by ultra high pressure (UHP) extraction and composition analysis. Compared with extracts obtained by leaching and ultrasonic extraction, the UHP extracts had enormous enhancement of their extraction yield, free radical (1,1-diphenyl-2-picrylhydrazyl) and superoxide anion radical scavenging activities and total phenolic content and these UHP extracts showed pressure and time-dependent increase of activities and content. Composition analysis by HPLC, main component of P. densiflora root extracts was taxifolin-3-glucoside, and each extract content (mg/g) was in the order: UHP 100. MPa 3. h extract (142.4 ± 1.3) > UHP 100. MPa 1. h extract (84.7 ± 2.1) > UHP 50. MPa 1. h extract (69.7 ± 1.8) > ultrasonic 1. h extract (48.6 ± 2.5) > leaching 1. h extract (42.5 ± 1.1). When UHP extraction, the taxifolin-3-glucoside content was increased and also these results revealed pressure and time-dependent increase. Taken together, UHP extraction is a potentially useful method for the pharmaceutical, cosmeceutical, functional food industries, because it improves the antioxidative effects of P. densiflora root. © 2011 The Korean Society of Industrial and Engineering Chemistry. Source


Chrysanthemum zawadskii extract (CZE) was investigated to determine its effects on the transactivation activity of peroxisome proliferator-activated receptors (PPAR)-responsive element (PPRE) and activity of anti-inflammatory for improvement of skin barrier function. The treatment with CZE resulted in a significant increase in the transactivation activity of PPRE such as PPAR-α and suppression in the TNF-α-, IL-6-induced NF-κB luciferase activity and NO production. In addition, CZE promotes the expression of protein related to cornified envelope (CE) formation such as involucrin. Therefore, these results indicate that CZE can restore skin barrier homeostasis and is suggested to be an appropriate skin therapeutic agent. © 2014, Korean Institute of Chemical Engineers, Seoul, Korea. Source


Joo C.G.,Morechem Corporation | Lee K.H.,Morechem Corporation | Park C.,Morechem Corporation | Joo I.W.,Catholic Kwandong University | And 2 more authors.
Journal of Industrial and Engineering Chemistry | Year: 2012

Antioxidants are indispensable for protecting skin cell membranes against oxidative injury caused by reactive oxygen species and other free radicals. Likewise, phenolic compounds are also important barriers to such oxidative injury. In this study, we examined the correlation between increased antioxidation and the contents of phenolic compounds and amino acids in Camellia sinensis leaf extracts obtained by ultra-high pressure extraction. Compared with extracts obtained by leaching and ultrasonic extraction, to evaluate whether this method can improve the pharmacologic effects of C. sinensis leaves. The ultra-high pressure (50 and 100. MPa) extracts had tremendous enhancement of their extraction yield, 1,1-diphenyl-2-picrylhydrazyl (free radical)-scavenging activity and superoxide anion radical scavenging activity, as well as the total phenolic, amino acid, and caffeine contents. The antioxidative effects were promoted not only by the phenolic compounds contents but also by other factors such as the amino acids content in the C. sinensis leaf extracts. These effects showed pressure-dependent increase with the enhanced phenolic compound and amino acid contents of the ultra high pressure extracts. Taken together, ultra high pressure extraction is a potentially useful method for the pharmaceutical, cosmetic, and food industries, because it improves the antioxidative effects of C. sinensis leaves. © 2011 The Korean Society of Industrial and Engineering Chemistry. Source


Atopic dermatitis,which is related to dermatologic disorders and is associatedwith skin barrier dysfunction, represents an epidemic problem demanding effective therapeutic strategies. In the present study, we showed that the treatment with Eruca sativa extract resulted in a significant increase in the transactivation activity of peroxisome proliferator-activated receptor (PPAR) response element such as PPAR-α and suppression in the expression of inflammatory cytokine and antimicrobial peptides. In addition, E. sativa extract promotes the expression of filaggrin related to skin barrier protection. Quercetin and isorhamnetin, flavonoids' constituents of E. sativa, also promoted PPAR-α activity. These results indicate that E. sativa extract may be an appropriate material for improving skin barrier function as a skin therapeutic agent for atopic dermatitis. Copyright © 2014 John Wiley & Sons, Ltd. Source


Lim J.Y.,Catholic University of Korea | Oh J.H.,Catholic University of Korea | Jung J.R.,Ellead Co. | Kim S.M.,Catholic University of Korea | And 3 more authors.
Journal of Neuro-Oncology | Year: 2010

Mounting evidence suggests that lipoxygenase (LO)-catalyzed products may play a key role in the development and progression of human cancers. In this study, we analyzed the effects of a 5-LO inhibitor, which inhibits the conversion of arachidonic acid to leukotrienes, on cell proliferation and apoptosis in human malignant glioma cells, including 5-LO-expressing cells U-87MG, A172 and 5-LO non-expressing cell U373. Growth of U-87MG and A172 cells, but not that of U373 cells, was inhibited in a dose-dependent manner by treatment with MK886. Similarly, specific 5-LO silencing by small interfering RNA reduced the growth of U-87MG and A172 cells. MK886 treatment reduced 5-LO activity independently of 5-LO-activating protein (FLAP) in human malignant glioma cells. MK886 treatment also induced cell apoptosis, measured by DNA fragmentation and nuclear condensation, in U-87MG and A172 cells but there were no signs in U373 cells. Moreover, this treatment reduced ERKs phosphorylation and anti-apoptotic molecule Bcl-2 expression, and increased Bax expression in U-87MG and A172 cells. In summary, our results show there is a link between the 5-LO expression status and the extent of MK886-inhibited cell proliferation and apoptosis. Taken together, this study suggest that 5-LO is a possible target for treating patients with gliomas, and 5-LO inhibition might be potent therapy for patients with 5-LO-expressing malignant gliomas. © Springer Science+Business Media, LLC. 2009. Source

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