Entity

Time filter

Source Type

Gainesville, FL, United States

Allred L.K.,ELISA Technologies Inc. | Voyksner J.A.S.,Immunogenics | Voyksner R.D.,LCMS Ltd
Journal of AOAC International | Year: 2014

To meet the need for the detection and quantitation of barley gluten in beer qualitative screening and quantitative immunoassays based on the monoclonal antigluten antibody 401/21 (Skerritt) were validated in a single laboratory. Sample replicates were tested at each stage of beer production using multiple yeast strains and methods of end-stage protein removal. Quantitation was performed using barley-specific standards based on barley flour extracts. Immunoassay results were confirmed using LC/MS/MS for barley-specific peptides. The LOD for the qualitative screening test was 5 mg/L barley gluten. Recovery for the barley-spiked worts ranged from 81 to 128% in the quantitative ELISA assay; the LOD was <1 mg/L, and the LOQ was 5 mg/L. Both screening and confirmation methods were found to be fit for the purposes of detection of low levels of barley gluten in beer. Source


Trademark
ELISA Technologies Inc. | Date: 2009-01-13

Chemical test kits for specific food proteins for laboratory or research use.


Trademark
ELISA Technologies Inc. | Date: 2009-01-13

Chemical test kits for specific food proteins for laboratory or research use.


Trademark
ELISA Technologies Inc. | Date: 2008-07-22

Chemical test kits for specific food proteins for laboratory or research use.


Allred L.K.,ELISA Technologies Inc. | Park E.S.,ELISA Technologies Inc. | Popping B.,Eurofins | Williams K.,U.S. Food and Drug Administration | Marrow T.,Laboratory Services Agriculture and Food Laboratory
Journal of AOAC International | Year: 2012

The EZ Gluten® assay is a rapid immunochromatographic screening method for qualitative detection of gluten in raw and cooked foods and beverages and on environmental surfaces. This AOAC Performance Tested MethodSM study evaluated the EZ Gluten assay as an effective method for the detection of gluten in four selected matrixes: rice flour, cooked dough, beer, and dog food. In addition, the method was evaluated for its effectiveness in detecting gluten contamination of ≥1 μg/2 in.2 (25 cm2) stainless steel surface area. The EZ Gluten demonstrated 100% specificity [probability of detection (POD) 0.00, confidence interval (CI) 0.00-0.01] and 99% sensitivity (POD 0.99, CI 0.97-0.995) at the 10 ppm level for all four matrixes, and 100% specificity (POD 0.00, CI 0.00-0.11) and sensitivity (POD 1.00, CI 0.886-1.00) at the 1 mg level on the stainless steel surface. Independent laboratory testing confirmed the internal validation results in one matrix and on the stainless steel surface. Lot-to-lot, stability, and robustness studies provided evidence that the EZ Gluten is a rugged, consistent method for the detection of gluten at levels as low as 10 ppm. © 2012 Publishing Technology. Source

Discover hidden collaborations