Thompson R.F.,University of Leeds |
Walker M.,MLW Consulting |
Siebert C.A.,Electronic Bio Imaging Center |
Muench S.P.,University of Leeds |
Ranson N.A.,University of Leeds
Methods | Year: 2016
Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150. kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a 'resolution revolution', owing to both advances in imaging hardware, image processing software, and improvements in sample preparation, leading to growing number of researchers using cryo-EM as a research tool. However, cryo-EM is still a rapidly growing field, with unique challenges. Here, we summarise considerations for imaging of a range of specimens from macromolecular complexes to cells using EM. © 2016 The Authors.