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Jeong E.J.,Seoul National University | Yang H.,Seoul National University | Kim S.H.,ElcomScience Co. | Kang S.Y.,Chonnam National University | And 2 more authors.
Food and Chemical Toxicology | Year: 2011

The excessive and prolonged nitric oxide (NO) production has been linked to various inflammatory diseases as well as tumourigenesis. On the search for anti-inflammatory and anti-cancer compounds from the medicinal plants, the methanolic extract of Euonymus alatus (Thunb.) Sieb. (Celastraceae) was found to have significant inhibitory activity on NO production in lipopolysaccharide (LPS)-stimulated BV2 microglia cells. Hence, we attempted to isolate the inhibitory constituent of E. alatus leaves and twigs on NO production. Thirteen compounds including two new glycerol derivates (1, 2), two C13 isoprenoids (3, 4), two phenolics (5, 6) and seven flavonoids (7-13) were isolated, and the structures of 1-13 were elucidated by extensive 1D and 2D spectroscopic methods. The isolated compounds significantly inhibited NO production induced by LPS in BV2 microglia cells. © 2011 Elsevier Ltd.


PubMed | Elcom Science Co., Seoul National University and U.S. National Cancer Institute
Type: Journal Article | Journal: Bioscience, biotechnology, and biochemistry | Year: 2015

Alzheimers disease (AD) is a neurodegenerative disease induced by cholinergic neuron damage or amyloid-beta aggregation in the basal forebrain region and resulting in cognitive disorder. We previously reported on the neuroprotective effects of Betula platyphylla bark (BPB) in an amyloid-beta-induced amnesic mouse model. In this study, we obtained a cognitive-enhancing compound by assessing results using a scopolamine-induced amnesic mouse model. Our results show that oral treatment of mice with BPB and betulin significantly ameliorated scopolamine-induced memory deficits in both passive avoidance and Y-maze tests. In the Morris water maze test, administration of BPB and betulin significantly improved memory and cognitive function indicating the formation of working and reference memories in treated mice. Moreover, betulin significantly increased glutathione content in mouse hippocampus, and the increase was greater than that from betulinic acid treatment. We conclude that BPB and its active component betulin have potential as therapeutic, cognitive enhancer in AD.


Lee K.Y.,Seoul National University | Jeong E.J.,Gyeongnam National University of Science and Technology | Huh J.,Seoul National University | Cho N.,Seoul National University | And 5 more authors.
Phytomedicine | Year: 2012

Diarylheptanoids have been the center of the intensive research efforts for Alzheimer's disease and other neurodegenerative diseases. The present study aimed to determine the effect of the standardized extract of B. platyphylla bark and its major diarylheptanoids in scopolamine-induced amnesic mice through cyclic AMP response element-binding protein (CREB) activation. Oral administration of the standardized extract of B. platyphylla bark (100 mg/kg body weight), aceroside VIII (1 mg/kg body weight) and platyphylloside (1 or 2 mg/kg body weight) significantly ameliorated scopolamine-induced amnesia in passive avoidance test. CREB phosphorylation and brain-derived neurotrophic factor (BDNF) expression in the cortex and hippocampus of the scopolamine-treated mice were markedly increased by the treatment of the standardized extract of B. platyphylla bark and platyphylloside. The standardized extract of B. platyphylla bark and its major diarylheptanoids also significantly protected HT22 cells against neurotoxicity induced by glutamate insult. The standardized extract of B. platyphylla bark and platyphylloside may ameliorate memory deficits by activating the CREB-BDNF pathway and prevent a neurodegeneration by inhibiting neuronal cell death. © 2012 Elsevier GmbH.


Lee M.A.,Seoul National University | Lee H.K.,ElcomScience Co. | Kim S.H.,ElcomScience Co. | Kim Y.C.,Seoul National University | Sung S.H.,Seoul National University
Planta Medica | Year: 2010

A methanolic extract of Alnus firma barks (Betulaceae) significantly attenuated nitric oxide production in lipopolysaccharide-stimulated BV2 microglia. Two new compounds, characterized as 4-hydroxy-2,6-dimethoxyphenyl 6-O-syringoyl-β-D-glucopyranoside (1) and 4-hydroxy-2,6-dimethoxyphenyl 6-O-vanilloyl-β-D-glucopyranoside (2), were isolated from the barks of A. firma using bioactivity-guided fractionation, together with two known phenolic glycosides (3, 4) and 11 known diarylheptanoids (515). Among these compounds, 14 and 611 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 10μ M to 100μ M. © Georg Thieme Verlag KG Stuttgart - New York.


Kim S.H.,Elcomscience Co. | Park J.H.,Seoul National University | Kim T.B.,Seoul National University | Lee H.H.,Seoul National University | And 3 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2010

The methanolic extract of the bark of Betula platyphylla was found to suppress antigen mediated degranulation of RBL-2H3 cells. Four arylbutanoids (1-4) and eight diarylhepatonoids (5-12) were isolated from the methanolic extract using bioassay-guided fractionation. Among them, compounds 4 and 12 were isolated and assigned for the first time. Compounds 1, 2, 3, 5, 10, and 12 showed remarkable inhibitory activity against the degranulation of RBL-2H3 by antigen stimulation in a dose dependent manner at the concentrations ranging from 10 μM to 100 μM. © 2010 Elsevier Ltd. All rights reserved.


Kim S.H.,Yonsei University | Jeon B.J.,Elcomscience Co. | Kim D.H.,Elcomscience Co. | Kim T.I.,Elcomscience Co. | And 6 more authors.
Journal of Medicinal Food | Year: 2012

The protective activity of prickly pear cactus (Opuntia ficus indica var. saboten) fruit juice and its main constituent, betanin, were evaluated against stress-induced acute gastric lesions in rats. After 6h of water immersion restraint stress (WIRS), gastric mucosal lesions with bleeding were induced in Sprague-Dawley rats. Pretreatment of a lyophilized powder containing O. ficus indica var. saboten fruit juice and maltodextrin (OFSM) and betanin significantly reduced stress lesions (800-1600mg/kg). Both OFSM and betanin effectively prevented the decrease in gastric mucus content as detected by alcian blue staining. In addition, OFSM significantly suppressed WIRS-induced increases in the level of gastric mucosal tumor necrosis factor-α and myeloperoxidase (MPO). Betanin alone was only effective in decreasing MPO. These results revealed the protective activity of OFSM against stress-induced acute gastric lesions and that betanin may contribute to OFSM's gastric protective activity, at least in part. When OFSM and betanin were taken together, OFSM exerted gastroprotective activity against stress-induced gastric lesions by maintaining gastric mucus, which might be related to the attenuation of MPO-mediated damage and proinflammatory cytokine production. © Copyright 2012, Mary Ann Liebert, Inc.


Yang H.,Seoul National University | Cho Y.W.,Seoul National University | Kim S.H.,Elcomscience Co. | Kim Y.C.,Seoul National University | Sung S.H.,Seoul National University
Phytochemistry | Year: 2010

Sixteen (1-16) triterpenoidal saponins were isolated from the roots of Pulsatilla koreana, of which four were determined as the previously unknown 23-hydroxy-3β-[(O-α-L-arabinopyranosyl)oxy]lup-20(29)-en-28-oic acid 28-O-β-D-glucopyranosyl ester (1), 23-hydroxy-3β-[(O-α-L- rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl)oxy]lup-20(29)-en-28-oic acid 28-O-β-D-glucopyranosyl ester (2), 3β-[(O-α-L- rhamnopyranosyl-(1 → 2)-α-L-arabinopyranosyl)oxy]lup-20(29)-en-28-oic acid 28-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester (3), and 3β-[(O-α-L-rhamnopyranosyl-(1 → 2)-O-[β-D- glucopyranosyl-(1 → 4)]-α-L-arabinopyranosyl)oxy]lup-20(29)-en-28-oic acid 28-O-α-L-rhamnopyranosyl-(1 → 4)-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl ester (4), respectively, based on spectroscopic analysis. The inhibition of the lipopolysaccharide-induced nitric oxide production of sixteen isolated compounds was evaluated in RAW 264.7 cells at concentrations ranging from 1 μM to 100 μM. © 2010 Elsevier Ltd. All rights reserved.


Kim S.H.,Elcomscience Co. | Kim H.K.,Korea Institute of Oriental Medicine | Yang E.S.,Seoul National University | Lee K.Y.,Seoul National University | And 3 more authors.
Separation and Purification Technology | Year: 2010

Method development based on pressurized liquid extraction technique was investigated for the extraction of spicatoside A from Liriope platyphylla. Determination of spicatoside A was carried out by high performance liquid chromatography coupled with charged aerosol detector. Central composite design consisting of 15 experimental points in triplicate was applied to evaluated the effects of three independent variables, namely extraction time (min), extraction temperature (°C) and ethanol concentration (%) on the acquired content of spicatoside A. Response surface analysis indicated that the highest predicted content of spicatoside A might be 0.0161% of the optimized extract at extraction time 20 min, extraction temperature 130 °C and ethanol concentration 86%. Under this optimal condition, the experimental value was 0.0157%, which agrees with the predicted one by analysis of variance. © 2009 Elsevier B.V. All rights reserved.


PubMed | Yonsei University, Elcom Science Co., Chung - Ang University and Seoul National University
Type: Journal Article | Journal: Journal of chromatographic science | Year: 2014

Micelle-mediated extraction offers a convenient alternative to conventional extraction systems. A new method based on micelle-mediated extraction was developed for the separation and determination of dibenzocyclooctadiene lignans from Schisandra chinensis by high-performance liquid chromatography with photodiode array detection. Various experimental conditions using the micelle-mediated method were investigated to evaluate the extraction process. Ethylene glycol monoalkyl ether (Genapol X-080), a non-ionic surfactant oligoethylene glycol monoalkyl ether, was chosen as the extract solvent. The chromatographic separation was accomplished on a Shiseido Capcell Pak C18 analytical column (250 4.6mm i.d., 5 m particle diameter), detected by ultraviolet absorption at 254 nm. The isocratic elution was achieved with a mobile phase composed of water-acetonitrile-formic acid (70:30:0.1) at a flow rate of 0.6 mL/min. The method was optimized and fully validated against dibenzocyclooctadiene lignans (schizandrin, gomisin A and gomisin N). With 15% Genapol X-080, a liquid to solid ratio of 100:1 (mL/g) and ultrasonic-assisted extraction for 60 min, the extraction percentage of total dibenzocyclooctadiene lignans reached the highest value. The non-ionic surfactant Genapol X-080 solution is an effective alternative for the extraction of bioactive lignans from S. chinensis.


PubMed | Ajou University, Elcom Science Co., Konkuk University and Seoul National University
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2014

Alzheimers disease (AD) is closely associated with amyloid (A)-induced neurotoxicity and oxidative stress in the brain. Betula platyphylla, which has been used to treat various oxidative-stressed related diseases, has recently received attention for its preventive activity on age-related neurodegenerative diseases. In this study, we attempted to investigate the effects of B.platyphylla bark (BPB-316) on A(1-42)-induced neurotoxicity and memory impairment. Oral treatment using BPB-316 significantly attenuated A-induced memory impairment which was evaluated by behavioral tests including the passive avoidance, Y-maze and Morris water maze test. BPB-316 also inhibited the elevation of -secretase activity accompanying the reduced A(1-42) levels in the hippocampus of the brain. Furthermore, BPB-316 significantly decreased the acetylcholinesterase activity and increased the glutathione content in the hippocampus. In addition, we confirmed that the expression of both cAMP responsive element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) in the hippocampus of A(1-42)-injected mice were markedly upregulated by the treatment of BPB-316. Our data suggest that the extracts of B.platyphylla bark might be a potential therapeutic agent against AD.

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