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Seoul, South Korea

Kim S.H.,Elcom Science Co. | Park J.H.,Seoul National University | Kim T.B.,Seoul National University | Lee H.H.,Seoul National University | And 3 more authors.
Bioorganic and Medicinal Chemistry Letters

The methanolic extract of the bark of Betula platyphylla was found to suppress antigen mediated degranulation of RBL-2H3 cells. Four arylbutanoids (1-4) and eight diarylhepatonoids (5-12) were isolated from the methanolic extract using bioassay-guided fractionation. Among them, compounds 4 and 12 were isolated and assigned for the first time. Compounds 1, 2, 3, 5, 10, and 12 showed remarkable inhibitory activity against the degranulation of RBL-2H3 by antigen stimulation in a dose dependent manner at the concentrations ranging from 10 μM to 100 μM. © 2010 Elsevier Ltd. All rights reserved. Source

Lee K.Y.,Seoul National University | Jeong E.J.,Gyeongnam National University of Science and Technology | Huh J.,Seoul National University | Cho N.,Seoul National University | And 5 more authors.

Diarylheptanoids have been the center of the intensive research efforts for Alzheimer's disease and other neurodegenerative diseases. The present study aimed to determine the effect of the standardized extract of B. platyphylla bark and its major diarylheptanoids in scopolamine-induced amnesic mice through cyclic AMP response element-binding protein (CREB) activation. Oral administration of the standardized extract of B. platyphylla bark (100 mg/kg body weight), aceroside VIII (1 mg/kg body weight) and platyphylloside (1 or 2 mg/kg body weight) significantly ameliorated scopolamine-induced amnesia in passive avoidance test. CREB phosphorylation and brain-derived neurotrophic factor (BDNF) expression in the cortex and hippocampus of the scopolamine-treated mice were markedly increased by the treatment of the standardized extract of B. platyphylla bark and platyphylloside. The standardized extract of B. platyphylla bark and its major diarylheptanoids also significantly protected HT22 cells against neurotoxicity induced by glutamate insult. The standardized extract of B. platyphylla bark and platyphylloside may ameliorate memory deficits by activating the CREB-BDNF pathway and prevent a neurodegeneration by inhibiting neuronal cell death. © 2012 Elsevier GmbH. Source

Kim S.H.,Elcom Science Co. | Kim H.K.,Korea Institute of Oriental Medicine | Yang E.S.,Seoul National University | Lee K.Y.,Seoul National University | And 3 more authors.
Separation and Purification Technology

Method development based on pressurized liquid extraction technique was investigated for the extraction of spicatoside A from Liriope platyphylla. Determination of spicatoside A was carried out by high performance liquid chromatography coupled with charged aerosol detector. Central composite design consisting of 15 experimental points in triplicate was applied to evaluated the effects of three independent variables, namely extraction time (min), extraction temperature (°C) and ethanol concentration (%) on the acquired content of spicatoside A. Response surface analysis indicated that the highest predicted content of spicatoside A might be 0.0161% of the optimized extract at extraction time 20 min, extraction temperature 130 °C and ethanol concentration 86%. Under this optimal condition, the experimental value was 0.0157%, which agrees with the predicted one by analysis of variance. © 2009 Elsevier B.V. All rights reserved. Source

Kim D.H.,Elcom Science Co. | Park J.H.,Elcom Science Co. | Kim Y.C.,Seoul National University | Sung S.H.,Seoul National University | Kim S.H.,Yonsei University
Natural Product Sciences

A new approach for similarity analysis of chemical profiles of Scrophularia buergeriana was developed. The roots of S. buergeriana collected in Andong, Korea were analyzed using HPLC-DAD and the peak areas of the chromatograms were used to construct the chemical profiles of S. buergeriana. The analytical conditions were validated for specificity, precision and repeatability. The standard chemical profiles were established from the chromatograms of nine standard analytical extracts from three batches. The correlation coefficients of peak areas of characteristic compounds between the chromatograms of the standard and test samples were calculated for quantitative expression of the chemical profiles. The results showed that the HPLC chemical profiles of the samples of same geographic origin were relatively consistent. This new approach could be applied to the quality assessment of herbal medicines with complex chemical compositions. Source

Lee M.A.,Seoul National University | Lee H.K.,Elcom Science Co. | Kim S.H.,Elcom Science Co. | Kim Y.C.,Seoul National University | Sung S.H.,Seoul National University
Planta Medica

A methanolic extract of Alnus firma barks (Betulaceae) significantly attenuated nitric oxide production in lipopolysaccharide-stimulated BV2 microglia. Two new compounds, characterized as 4-hydroxy-2,6-dimethoxyphenyl 6-O-syringoyl-β-D-glucopyranoside (1) and 4-hydroxy-2,6-dimethoxyphenyl 6-O-vanilloyl-β-D-glucopyranoside (2), were isolated from the barks of A. firma using bioactivity-guided fractionation, together with two known phenolic glycosides (3, 4) and 11 known diarylheptanoids (515). Among these compounds, 14 and 611 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglial cells at concentrations ranging from 10μ M to 100μ M. © Georg Thieme Verlag KG Stuttgart - New York. Source

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