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News Article | December 1, 2016

Salmonella, a gram-negative facultative rod-shaped bacterium, is found in human and animal intestines. Most people get infected with salmonella by eating contaminated food such as raw meat, infected poultry and seafood, raw eggs, and contaminated fruits and vegetables. Salmonella infection causes diseases and conditions such as typhoid, paratyphoid, and food poisoning. Major symptoms of salmonella infection include diarrhea, fever, dehydration, and abdominal cramps that usually occur within 8 to 72 hours after ingesting contaminated food. Most people recover without treatment. However, sometimes diarrhea and dehydration may become severe and the patient may need medical attention. Elderly people, infants, and people who have a weak immune system due to disease such as AIDS, sickle cell disease, malaria, and corticosteroids get affected more easily by salmonella infection. The salmonella testing procedure includes detection and isolation, and confirmation and identification of the bacteria. In the detection and isolation stage, animal feces and water sources are tested for the bacteria. Moreover, large number of food ingredients and food products are routinely tested by the food industry, especially meat products, eggs, and dairy products for the detection of salmonella in these items. Salmonella testing methods include traditional and rapid detection methods. It takes 3 to 5 days to detect the presence of salmonella in a source using the tradititional methods, while the rapid detection method takes up to 48 hours to the detect the presence of salmonella. Request TOC (desk of content material), Figures and Tables of the report: The global salmonella testing market is categorized based on various technologies used to detect the bacteria, food type, and end user. Based on technology, the report covers traditional and rapid technologies. Rapid technology includes polymerase chain reaction (PCR)-based testing, immunoassay-based testing, convenience-based testing, and DNA hybridization. Based on food type, the market comprises meat and poultry, dairy products, fruits and vegetables, and others. Based on end user, the market covers hospitals, public health labs, physician offices, and commercial labs. In terms of geography, North America has the largest market for salmonella testing, followed by Europe. This is due to implementation of stringent food safety regulations that track every testing procedure of food materials during every stage of food production and processing, rise in foodborne diseases, technological advancements in testing procedures, and improved healthcare infrastructure in the region. The salmonella testing market in Asia is expected to experience a high growth rate over the next few years due to developing healthcare infrastructure, increasing prevalence of foodborne diseases, and growing research and development activities on salmonella testing in the region. Moreover, growing demographics and economies in developing countries such as India and China are also expected to drive the salmonella testing market in Asia. However, lack of food control infrastructure and resources in developing countries inhibits the growth of the market. Rising awareness about food- and waterborne diseases and growing food industry are some of the major drivers of the salmonella testing market. Also, implementation of food safety regulations across the world and technological innovations in bacterial detection technologies is fuelling the growth of this market. Increasing number of mergers and acquisitions, rising number of collaborations and partnerships, and new product launches are some of the latest trends in the global salmonella testing market. The major companies operating in this market worldwide are Affymetrix, Inc., Abbott, Becton, Dickinson and Company, F. Hoffmann-La Roche AG, Siemens AG, Bio-Rad, DiaSorin S.p.A., bioMérieux, Diamedix Corporation, Eiken Chemical Co., Ltd., Fujirebio Diagnostics, Inc., Hologic, Inc. and Enzo Biochem, Inc.

Francois P.,University of Geneva | Tangomo M.,University of Geneva | Hibbs J.,University of Geneva | Bonetti E.-J.,University of Geneva | And 4 more authors.
FEMS Immunology and Medical Microbiology | Year: 2011

We evaluated the robustness of loop-mediated isothermal amplification (LAMP) of DNA for bacterial diagnostic applications. Salmonella enterica serovar Typhi was used as the target organism and compared with a real-time quantitative PCR (qPCR) for testing assay performance and reproducibly, as well as the impact of pH and temperature stability. This isothermal amplification method appeared to be particularly robust across 2 pH units (7.3-9.3) and temperature values (57-67°C). The detection limit was comparable to that observed using optimized home-brew qPCR assays. The specificity of the amplification reaction remained high even at temperatures markedly different from the optimal one. Exposing reagents to the ambient temperature during the preparation of the reaction mixture as well as prolonging times for preparing the amplification reaction did not yield false-positive results. LAMP remained sensitive and specific despite the addition of untreated biological fluids such as stool or urine that commonly inhibit PCR amplification. Whereas the detection of microorganisms from whole blood or a blood-culture medium typically requires extensive sample purification and removal of inhibitors, LAMP amplification remained more sensitive than conventional qPCR when omitting such preparatory steps. Our results demonstrate that LAMP is not only easy to use, but is also a very robust, innovative and powerful molecular diagnostic method for both industrialized and developing countries. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd.

Notes:  Production, means the output of Microbiology Culture  Revenue, means the sales value of Microbiology Culture This report studies Microbiology Culture in Global Market, especially in North America, Europe, China, Japan, Southeast Asia and India, focuses on top manufacturers in global market, with Production, price, revenue and market share for each manufacturer, covering Siemens Healthcare  Becton, Dickinson and Company  bioMerieux S.A.  Danaher Corporation  Bio-Rad Laboratories, Inc.  Himedia Laboratories Pvt. Ltd.  Thermo Fisher Scientific Inc.  Abbott Laboratories  Eiken Chemical Co.,Ltd.  Carthera Company  Market Segment by Regions, this report splits Global into several key Regions, with production, consumption, revenue, market share and growth rate of Microbiology Culture in these regions, from 2011 to 2021 (forecast), like  North America  Europe  China  Japan  Southeast Asia  India  Split by product type, with production, revenue, price, market share and growth rate of each type, can be divided into  Type I  Type II  Type III  Split by application, this report focuses on consumption, market share and growth rate of Microbiology Culture in each application, can be divided into  Application 1  Application 2  Application 3 Global Microbiology Culture Market Research Report 2016  1 Microbiology Culture Market Overview  1.1 Product Overview and Scope of Microbiology Culture  1.2 Microbiology Culture Segment by Type  1.2.1 Global Production Market Share of Microbiology Culture by Type in 2015  1.2.2 Type I  1.2.3 Type II  1.2.4 Type III  1.3 Microbiology Culture Segment by Application  1.3.1 Microbiology Culture Consumption Market Share by Application in 2015  1.3.2 Application 1  1.3.3 Application 2  1.3.4 Application 3  1.4 Microbiology Culture Market by Region  1.4.1 North America Status and Prospect (2011-2021)  1.4.2 Europe Status and Prospect (2011-2021)  1.4.3 China Status and Prospect (2011-2021)  1.4.4 Japan Status and Prospect (2011-2021)  1.4.5 Southeast Asia Status and Prospect (2011-2021)  1.4.6 India Status and Prospect (2011-2021)  1.5 Global Market Size (Value) of Microbiology Culture (2011-2021) 2 Global Microbiology Culture Market Competition by Manufacturers  2.1 Global Microbiology Culture Production and Share by Manufacturers (2015 and 2016)  2.2 Global Microbiology Culture Revenue and Share by Manufacturers (2015 and 2016)  2.3 Global Microbiology Culture Average Price by Manufacturers (2015 and 2016)  2.4 Manufacturers Microbiology Culture Manufacturing Base Distribution, Sales Area and Product Type  2.5 Microbiology Culture Market Competitive Situation and Trends  2.5.1 Microbiology Culture Market Concentration Rate  2.5.2 Microbiology Culture Market Share of Top 3 and Top 5 Manufacturers  2.5.3 Mergers & Acquisitions, Expansion 3 Global Microbiology Culture Production, Revenue (Value) by Region (2011-2016)  3.1 Global Microbiology Culture Production and Market Share by Region (2011-2016)  3.2 Global Microbiology Culture Revenue (Value) and Market Share by Region (2011-2016)  3.3 Global Microbiology Culture Production, Revenue, Price and Gross Margin (2011-2016)  3.4 North America Microbiology Culture Production, Revenue, Price and Gross Margin (2011-2016)  3.5 Europe Microbiology Culture Production, Revenue, Price and Gross Margin (2011-2016)  3.6 China Microbiology Culture Production, Revenue, Price and Gross Margin (2011-2016)  3.7 Japan Microbiology Culture Production, Revenue, Price and Gross Margin (2011-2016)  3.8 Southeast Asia Microbiology Culture Production, Revenue, Price and Gross Margin (2011-2016)  3.9 India Microbiology Culture Production, Revenue, Price and Gross Margin (2011-2016) 4 Global Microbiology Culture Supply (Production), Consumption, Export, Import by Regions (2011-2016)  4.1 Global Microbiology Culture Consumption by Regions (2011-2016)  4.2 North America Microbiology Culture Production, Consumption, Export, Import by Regions (2011-2016)  4.3 Europe Microbiology Culture Production, Consumption, Export, Import by Regions (2011-2016)  4.4 China Microbiology Culture Production, Consumption, Export, Import by Regions (2011-2016)  4.5 Japan Microbiology Culture Production, Consumption, Export, Import by Regions (2011-2016)  4.6 Southeast Asia Microbiology Culture Production, Consumption, Export, Import by Regions (2011-2016)  4.7 India Microbiology Culture Production, Consumption, Export, Import by Regions (2011-2016) Wise Guy Reports is part of the Wise Guy Consultants Pvt. Ltd. and offers premium progressive statistical surveying, market research reports, analysis & forecast data for industries and governments around the globe. Wise Guy Reports understand how essential statistical surveying information is for your organization or association. Therefore, we have associated with the top publishers and research firms all specialized in specific domains, ensuring you will receive the most reliable and up to date research data available.

Christensen M.,Copenhagen University | Jacobsen S.,Copenhagen University | Ichiyanagi T.,EIKEN Chemical Company | Kjelgaard-Hansen M.,Copenhagen University
Veterinary Journal | Year: 2012

Major acute phase proteins (APPs) have proven diagnostically useful in dogs, cats and horses with routine use facilitated by commercially available automated heterologous assays. An automated assay applicable across all three species would highly facilitate further dissemination of routine use, and the aim of this study was to validate an automated latex agglutination turbidimetric immunoassay based on monoclonal anti-human serum amyloid A (SAA) antibodies for measurement of canine, feline and equine SAA. Serum samples from 60 dogs, 40 cats and 40 horses were included. Intra- and inter-assay imprecision, linearity and detection limit (DL) were determined to assess analytical performance. To assess clinical performance, equine and feline SAA measurements were compared with parallel measurements using a previously validated automated SAA assay in a method comparison setting, and by assessing overlap performance of canine SAA in healthy dogs and diseased dogs with and without systemic inflammation.Intra- and inter-assay CVs ranged between 1.9-4.6% and between 3.0-14.5%, respectively. Acceptable linearity within a clinically relevant range of SAA concentrations was observed for all three species. The DL was 1.06. mg/L. Method comparison revealed acceptable agreement of the two assays measuring feline and equine SAA, and the overlap performance of canine SAA was acceptable. The tested assay measured SAA in canine, feline and equine serum with analytical and overlap performance acceptable for clinical purposes so improving practical aspects of clinical APP application. The monoclonal nature of the antibodies suggests strong, long-term inter-batch performance stability. © 2012 Elsevier Ltd.

Notomi T.,Eiken Chemical Co. | Mori Y.,Eiken Chemical Co. | Tomita N.,Eiken Chemical Co. | Kanda H.,Eiken Chemical Co.
Journal of microbiology (Seoul, Korea) | Year: 2015

Loop-mediated isothermal amplification (LAMP), a newly developed gene amplification method, combines rapidity, simplicity, and high specificity. Several tests have been developed based on this method, and simplicity is maintained throughout all steps, from extraction of nucleic acids to detection of amplification. In the LAMP reaction, samples are amplified at a fixed temperature through a repetition of two types of elongation reactions occurring at the loop regions: self-elongation of templates from the stem loop structure formed at the 3'-terminal and the binding and elongation of new primers to the loop region. The LAMP reaction has a wide range of possible applications, including point-of-care testing, genetic testing in resource-poor settings (such as in developing countries), and rapid testing of food products and environmental samples.

Minekawa T.,Eiken Chemical Co. | Takehara S.,Eiken Chemical Co. | Takahashi M.,Jichi Medical University | Okamoto H.,Jichi Medical University
Clinical and Vaccine Immunology | Year: 2013

Hepatitis B virus (HBV) infections are sometimes overlooked when using commercial kits to measure hepatitis B virus surface antigen (HBsAg) due to their low sensitivities and reactivities to mutant strains of various genotypes. We developed an ultrasensitive bioluminescent enzyme immunoassay (BLEIA) for HBsAg using firefly luciferase, which is adaptable to a variety of HBsAg mutants, by combining four monoclonal antibodies with a polyclonal antibody against HBsAg. The measurement of seroconversion panels showed trace amounts of HBsAg during the early infection phase by the BLEIA because of its high sensitivity of 5 mIU/ml. The BLEIA detected HBsAg as early as did PCR in five of seven series and from 2.1 to 9.4 days earlier than commercial immunoassay methods. During the late infection phase, the BLEIA successfully detected HBsAg even 40 days after the disappearance of HBV DNA and the emergence of antibodies against HBsAg. The HBsAg BLEIA successfully detected all 13 recombinant HBsAg and 45 types of HBsAg mutants with various mutations within amino acids 90 to 164 in the S gene product. Some specimens had higher values determined by the BLEIA than those by a commercial chemiluminescent immunoassay; this suggests that such discrepancies were caused by the dissociation of preS1/preS2 peptides from the particle surface. With its highly sensitive detection of low-titer HBsAg, including various mutants, the HBsAg BLEIA is considered to be useful for the early diagnosis and prevention of HBV infection because of the shorter window of infection prior to detection, which facilitates early prediction of recurrence in HBV-infected individuals. Copyright © 2013, American Society for Microbiology.

Kotani K.,Jichi Medical University | Minami T.,Jichi Medical University | Abe T.,Eiken Chemical Co. | Sato J.,Eiken Chemical Co. | And 2 more authors.
Clinica Chimica Acta | Year: 2014

Background: White blood cell (WBC) count and C-reactive protein (CRP) level are the most common markers of inflammation. There is a growing need for point-of-care testing (POCT) of WBC and CRP, and more advances in convenient devices are required. We developed an analyzer-free POCT system for measuring WBC and CRP using a low volume blood sample. Methods: The POCT-WBC is based on the granulocyte esterase assay, while the POCT-CRP is based on the immunochromatographic assay. These kits were examined for precision as well as correlation with currently used popular commercial automated assays. The correlations were clinically analyzed in children with acute infection (n = 62; mean age 4.2. y). The correlations regarding the monitoring of values were further examined in several follow-up subjects. Results: The POCT-WBC and POCT-CRP kits demonstrated good precision. POCT-WBC exhibited a significantly close correlation with those of the control assay (r = 0.94, p. <. 0.05). The results of POCT-CRP also exhibited a significantly close correlation with those of the control assay (r = 0.94, p. <. 0.05). In the follow-up study, the results of the respective kits were similar to those of the control assays. Conclusions: The POCT-WBC and POCT-CRP are promising tools for assessing infection in clinical practice. © 2014 Elsevier B.V.

Eiken Chemical Co. | Date: 2015-11-18

A hemoglobin stabilizing storage solution comprising hemoglobin and 3-hydroxy-2,2-iminodisuccinic acid of Formula (2), or a salt thereof:

Eiken Chemical Co. | Date: 2010-07-07

There is provided a novel method of stabilizing a heme protein, which is effective against denaturation and degradation of a heme protein such as hemoglobin. A method of stabilizing a heme protein comprises allowing an iminocarboxylic acid or a salt thereof to coexist in a sample containing the heme protein. In the method, the iminocarboxylic acid or a salt thereof is preferably a compound of Formula (1):

Eiken Chemical Co. | Date: 2012-09-12

A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and Xs represent each a hydrogen atom, an alkali metal or an ammonium group.

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