Eighth Peoples Hospital of Guangzhou

Guangzhou, China

Eighth Peoples Hospital of Guangzhou

Guangzhou, China
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Zhou J.,First Peoples Hospital Of Foshan | Chen H.,Eighth Peoples Hospital Of Guangzhou | Yang C.,First Peoples Hospital Of Foshan | Zhong J.,First Peoples Hospital Of Foshan | And 2 more authors.
Neurochemical Research | Year: 2017

Despite the consensus that activation of TWIK-related spinal cord K+ (TRESK) might contribute to the pathogenesis of chronic pain, the specific mechanisms underlying the transfer and development of pain signals still remain obscure. In the present study, we validated that TRESK was expressed in neurons instead of glial cells. Furthermore, in the SNI model of neuropathic pain (NP), downregulation of TRESK in spinal cord neurons resulted in upregulation of connexin 36 (Cx36) and connexin 43 (Cx43), both being subtypes of gap junctions in the spinal cord, with gliocytes in the spinal cord activated ultimately. Compared with SNI rats, intrathecal injection of TRESK gene recombinant adenovirus significantly downregulated the expression levels of Cx36 and Cx43 and suppressed the activation of gliocytes in the spinal cord, with hyperalgesia significantly reduced. In conclusion, TRESK contributes to the pathogenesis of NP by upregulation of synaptic transmission and activation of gliocytes. © 2017 Springer Science+Business Media New York


Zhou J.,First Peoples Hospital of Foshan | Lin W.,First Peoples Hospital of Foshan | Chen H.,Eighth Peoples Hospital of Guangzhou | Fan Y.,Central Hospital of Panyu District | Yang C.,First Peoples Hospital of Foshan
Neuroscience | Year: 2016

The mechanism underlying neuropathic pain (NP) is complex and has not been fully elucidated. The TWIK-related spinal cord K+ (TRESK) is the major background potassium current in dorsal root ganglia (DRG), we found that mitogen-activated protein kinase (MAPK) signal pathway were activated in spinal cord accompanied by TRESK down regulation in response to NP. Therefore, we investigated whether TRESK mediates inflammation and apoptosis by MAPK pathway in the spinal cord of NP rats. SNI rats exhibited reduced TRESK expression in DRG and spinal cord and higher sensitivity to mechanical stimuli but no effect on thermal stimuli. Intrathecal injections of TRESK overexpressing adenovirus alleviated mechanical allodynia, inhibited phosphorylation of extracellular signal-regulated kinase (ERK) and p38, and decreased inflammatory reactions and apoptosis in the spinal cords of SNI rats. Down regulation of TRESK in DRG and spinal cord was detected in normal rats after intrathecal TRESK shRNA lentivirus injection, which induced mechanical allodynia but had no effect on pain thresholds for heat stimulation. Phosphorylated ERK and p38 were increased in the spinal cord. Intrathecal injection of an ERK antagonist (PD98059) and p38 antagonist (SB203580) prevented ERK and p38 activation in the spinal cord and mechanical allodynia induced by TRESK shRNA lentivirus. In conclusion, our study clearly demonstrated an important role for TRESK in NP and that TRESK regulation contributes to pain sensitivity mediates inflammation and apoptosis by ERK and p38 MAPK signaling in the spinal cord. © 2016 IBRO


Zhang J.,Eighth Peoples Hospital of Guangzhou | Zhang C.,Eighth Peoples Hospital of Guangzhou | Zeng C.,Eighth Peoples Hospital of Guangzhou | Yang H.,Eighth Peoples Hospital of Guangzhou | Meng Y.,Eighth Peoples Hospital of Guangzhou
Chinese Journal of Clinical Oncology | Year: 2016

Objective: To investigate the therapeutic effects of nucleoside analogues (acid) and transarterial chemoembolization (TACE) on hepatitis B-related primary liver cancer. Methods: Cases from Guangzhou Eighth People's Hospital were retrospectively analyzed between January 2011 and December 2015. A total of 174 patients were diagnosed with hepatitis B-related primary liver cancer. Patients were categorized based on needs and individual goals. The control group included patients who were classified based on needs (n=72), whereas the research group included patients who were classified based on individual goals (n=102). In the control group, 72 patients underwent TACE. In the research group, 102 patients received nucleoside analogue (acid) and TACE. Results: The two groups did not differ significantly in terms of basic clinical parameters, such as age, sex, tumor size, and laboratory examination (P>0.05). After treatment, the research group was compared with the control group. The liver function of patients in the research group significantly improved (P<0.05). AFP and CA199 levels significantly decreased after 12, 24, and 48 weeks of treatment in the research group (P< 0.05). The research group had HBVDNA unpredictable rates of 13.73%, 33.33%, 50.0%, and 76.47% after 4, 12, 24, and 48 weeks of treatment, respectively. The control group had HBVDNA unpredictable rates of 11.11%, 16.67%, 22.22%, and 25.00% after 12, 24, and 48 weeks of treatment, respectively. The HBVDNA unpredictable rate was significantly higher in the control group (P<0.05). The treatment effects in the research group were better than that of the control group (P<0.05). In the research group, the 1- and 3-year survival rates were 96.08% and 90.20%, respectively. In the control group, the 1- and 3-year survival rates were 83.33% and 63.89%, respectively. The difference in survival rates between the two groups was statistically significant (P<0.05). Conclusion: TACE based on combined nucleoside analogue (acid) and antiviral treatment improved ALT levels in patients with hepatitis B-related primary liver cancer and decreased AFP, CA199, and HBVDNA levels. Moreover, the HBVDNA unpredictable rate was modified to facilitate the TACE procedure, therefore improving the survival rates of patients and prolonging survival. Therefore, the therapeutic actions of antiviral treatment on hepatitis B-related primary liver cancer should be further investigated.


PubMed | First Peoples Hospital of Foshan, Eighth Peoples Hospital of Guangzhou and Central Hospital of Panyu District
Type: | Journal: Neuroscience | Year: 2016

The mechanism underlying neuropathic pain (NP) is complex and has not been fully elucidated. The TWIK-related spinal cord K


Cai H.-X.,Sun Yat Sen University | Cheng H.-L.,Sun Yat Sen University | Song J.-W.,Eighth Peoples Hospital of Guangzhou | Chen S.-Y.,Sun Yat Sen University
Experimental and Therapeutic Medicine | Year: 2014

The aim of the present study was to compare the root canal preparation ability of rotary nickel-titanium (NiTi) Hero 642 and K3 files in curved mandibular or maxillary molars. A total of 40 extracted mandibular molars with two separate mesial canals, an apical width of approximately size ≤15 and a root canal curvature of 15-30 ° were randomly divided into two groups and instrumented using Hero 642 (n=20) or K3 files (n=20). Canal straightening, working length, transportation, cross-sectional area, minimum dentin thickness and the canal angle curvature degree were examined, and a systematic review of the literature was conducted. No statistically significant differences were observed between the two groups with regard to the mean degree of straightening, mean change in working length, mean transportation, amount of dentin removed or remaining minimum dentin thickness (P>0.05). The canal angle curvature decreased in the two groups postoperatively. The systematic review identified six studies, and overall the two files performed similarly in the majority of categories examined. Therefore, the rotary NiTi Hero 642 and K3 files demonstrated comparable shaping abilities and maintenance of working length.


PubMed | Eighth Peoples Hospital of Guangzhou, Zhongshan Second Peoples Hospital, Sun Yat Sen University and Panyu Peoples Hospital
Type: Clinical Trial, Phase III | Journal: PloS one | Year: 2015

The optimal treatment of hepatitis C virus (HCV) genotype 6 is unclear owing to its limited geographic distribution. Because of a high predictive value of rapid virological response (RVR) for sustained virological response (SVR), we conducted an open-label randomized controlled trial to compare 24- and 48-week peginterferon/ribavirin combination therapy for patients with HCV genotype 6 in Southern China who achieved an RVR.Treatment-naive, non-cirrhotic patients with chronic hepatitis C genotype 6 were treated with pegylated interferon -2a (180 g/week) and ribavirin (800-1,200 mg, according to weight) for 4 weeks. Patients who achieved an RVR, which was defined as HCV RNA negativity at week 4 (<50 IU), were randomized to receive either an additional 20 or 44 weeks of treatment (24- and 48-week treatment groups, respectively). The primary outcome measure was SVR. From January 2011 to June 2014, 152(152/210, 72.4%) patients with HCV genotype 6a and RVR were randomized 1:1 to the 24- or 48-week treatment group. The SVR rates in the 24- and 48-week groups in the intention-to-treat analysis were 90.8% (69/76) and 88.2% (67/76), respectively; those in the per-protocol analysis were 95.7% (67/70) and 97.0% (64/66), respectively. More patients in the 48-week group had anemia (46.1% vs. 28.9%, P = 0.03), but other adverse events were comparable between the groups. The limitation of the present study was that only patients from Southern China were enrolled which may inhibit the extensive application of the findings.Twenty-four weeks of peginterferon/ribavirin combination therapy was non-inferior to 48 weeks in patients with HCV genotype 6a in Southern China who achieved an RVR.ClinicalTrials.gov NCT01263860.


PubMed | Eighth Peoples Hospital of Guangzhou and Sun Yat Sen University
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2014

The aim of the present study was to compare the root canal preparation ability of rotary nickel-titanium (NiTi) Hero 642 and K3 files in curved mandibular or maxillary molars. A total of 40 extracted mandibular molars with two separate mesial canals, an apical width of approximately size 15 and a root canal curvature of 15-30 were randomly divided into two groups and instrumented using Hero 642 (n=20) or K3 files (n=20). Canal straightening, working length, transportation, cross-sectional area, minimum dentin thickness and the canal angle curvature degree were examined, and a systematic review of the literature was conducted. No statistically significant differences were observed between the two groups with regard to the mean degree of straightening, mean change in working length, mean transportation, amount of dentin removed or remaining minimum dentin thickness (P>0.05). The canal angle curvature decreased in the two groups postoperatively. The systematic review identified six studies, and overall the two files performed similarly in the majority of categories examined. Therefore, the rotary NiTi Hero 642 and K3 files demonstrated comparable shaping abilities and maintenance of working length.


Xiao Y.-H.,Central South University | Xiao Y.-H.,Eighth Peoples Hospital of Guangzhou | Li X.-H.,Central South University | Tan T.,Central South University | And 11 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2011

Purpose: To identify methylation-silenced genes in acute myeloid leukemia (AML). Methods: Microarray analyses were performed in AML cell line HL-60 cells exposed to the demethylating agent 5-aza-2dC. The methylation status and expression of glioma pathogenesis-related protein 1 (GLIPR1), one of highly induced genes by demethylation, were further detected in six hematopoietic malignancy cell lines and 260 bone marrow samples from leukemia patients and nonmalignant diseases as control, as well as pre-treated and post-treated bone marrow samples from 24 complete remission AML patients received chemotherapy using MS-PCR, bisulfite DNA sequencing, RT-PCR, and Western blotting. Results: One hundred and nine genes were significantly induced by demethylation in HL-60 cells, 12 genes of which were confirmed by RT-PCR. GLIPR1, a tumor suppressor gene, was frequently methylation-silenced in AML cell lines and AML patients, but not in the other hematopoietic malignancy cell lines and patients. The frequencies of methylation-silenced GLIPR1 in the pre-treatment were significantly higher than those in the post-treatment in complete remission AML patients. Conclusion: We identify 109 genes induced by demethylation in HL-60 cells, and demonstrate that GLIPR1 is a methylation-silenced gene in the AML patients, and may serve as a marker for monitoring disease activity during therapy in the AML patients. The data provide the important information for studying the pathogenesis of AML and discovering the target genes of methylating agents. © 2011 Springer-Verlag.


Li Z.,Eighth Peoples Hospital of Guangzhou
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2011

To investigate the optimal perioperative management of HIV infection/AIDS in surgical patients in the department of gynecology and obstetrics. Fifty-seven surgical patients with HIV infection or AIDS were studies. Before the operations, the patients were treated with highly active anti-retroviral therapy (HAART), and the surgical approach was determined according to the level of CD4(+) T lymphocytes. The operations were performed strictly following the standard procedures with careful operation to reduce surgical injuries. Routine antibiotics were administered to prevent postoperative infections. The white blood cell count, hemoglobin and CD4(+) T lymphocytes recorded pre- and postoperatively were analyzed for evaluation of the perioperative management and therapeutic effects. With these perioperative managements, the white blood cells and CD4(+) T lymphocytes showed no significant changes after the operation (P>0.05) and no wound infections or opportunistic infections were found in these cases. Appropriate perioperative management can ensure surgical success in patients with HIV infection or AIDS in the department of gynecology and obstetrics.


PubMed | Eighth Peoples Hospital of Guangzhou
Type: Journal Article | Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2011

To investigate the optimal perioperative management of HIV infection/AIDS in surgical patients in the department of gynecology and obstetrics.Fifty-seven surgical patients with HIV infection or AIDS were studies. Before the operations, the patients were treated with highly active anti-retroviral therapy (HAART), and the surgical approach was determined according to the level of CD4(+) T lymphocytes. The operations were performed strictly following the standard procedures with careful operation to reduce surgical injuries. Routine antibiotics were administered to prevent postoperative infections. The white blood cell count, hemoglobin and CD4(+) T lymphocytes recorded pre- and postoperatively were analyzed for evaluation of the perioperative management and therapeutic effects.With these perioperative managements, the white blood cells and CD4(+) T lymphocytes showed no significant changes after the operation (P>0.05) and no wound infections or opportunistic infections were found in these cases.Appropriate perioperative management can ensure surgical success in patients with HIV infection or AIDS in the department of gynecology and obstetrics.

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