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Yang H.,Semel Institute for Neuroscience and Human Behavior | Thompson A.B.,Semel Institute for Neuroscience and Human Behavior | McIntosh B.J.,Eicom Corporation | Altieri S.C.,Semel Institute for Neuroscience and Human Behavior | And 2 more authors.
ACS Chemical Neuroscience | Year: 2013

Online microdialysis is a sampling and detection method that enables continuous interrogation of extracellular molecules in freely moving subjects under behaviorally relevant conditions. A majority of recent publications using brain microdialysis in rodents report sample collection times of 20-30 min. These long sampling times are due, in part, to limitations in the detection sensitivity of high performance liquid chromatography (HPLC). By optimizing separation and detection conditions, we decreased the retention time of serotonin to 2.5 min and the detection threshold to 0.8 fmol. Sampling times were consequently reduced from 20 to 3 min per sample for online detection of serotonin (and dopamine) in brain dialysates using a commercial HPLC system. We developed a strategy to collect and to analyze dialysate samples continuously from two animals in tandem using the same instrument. Improvements in temporal resolution enabled elucidation of rapid changes in extracellular serotonin levels associated with mild stress and circadian rhythms. These dynamics would be difficult or impossible to differentiate using conventional microdialysis sampling rates. © 2013 American Chemical Society. Source


Patent
Osaka University and Eicom Corporation | Date: 2013-03-11

A dialysis probe includes a tubular dialysis membrane sealed at its tip, a support tube coupled at a tip to a rear end of the dialysis membrane, a cap portion for securing a rear end of the support tube, an inlet conduit extending through the cap portion toward the tip of the dialysis membrane within a space to guide a perfusate into the space, an outlet conduit extending through the cap portion toward the tip of the dialysis membrane within the space to guide the perfusate in the space outside the space, and at least one air-exposure through-hole provided in the cap portion for maintaining the space at atmospheric pressure. The inlet conduit has a longer protruding length from the rear end of the support tube when compared to the outlet conduit.


Patent
Osaka University and Eicom Corporation | Date: 2011-04-19

Provided is a dialysis probe capable of stably performing an analysis with high accuracy for a long period of time even if the inflow and outflow rates of a perfusate are not completely equalized. A dialysis probe


Takeda S.,Osaka University | Sato N.,Osaka University | Ikimura K.,Eicom Corporation | Nishino H.,Eicom Corporation | And 2 more authors.
Neurobiology of Aging | Year: 2013

Behavioral and psychological problems are often observed in patients with dementia such as that associated with Alzheimer disease, and these noncognitive symptoms place an extremely heavy burden on the family and caregivers. Although it is well know that these symptoms often are triggered by infection of peripheral organs, the underlying mechanisms for these pathological conditions are still unclear. In this study, using an Alzheimer amyloid precursor protein (APP)-transgenic mouse, we analyzed behavioral changes and brain inflammatory response induced by peripheral administration of lipopolysaccharide. Application of a unique in vivo microdialysis system revealed that the increase in brain inflammatory cytokine (interleukin-6) level was significantly higher in APP-Tg than in wild-type mice after peripheral lipopolysaccharide injection, which was associated with more severe sickness behaviors. The blood-brain barrier became more permeable in APP-Tg mice during peripherally evoked inflammation, suggesting the increased vulnerability of the blood-brain barrier to inflammation in this animal model of Alzheimer's disease. These findings might provide insight into the pathogenesis of noncognitive symptoms in dementia and a basis to develop new therapeutic treatments for them. © 2013 Elsevier Inc. Source


Roy M.C.,Okinawa Institute of Science and Technology | Ikimura K.,Eicom Corporation | Nishino H.,Eicom Corporation | Naito T.,Okinawa Institute of Science and Technology
Analytical Biochemistry | Year: 2010

A high recovery microsampling probe based on microdialysis was devised. The new probe showed a high recovery (100%) of peptides in vitro at different perfusion flow rates (0.1-1.0 μl/min). At a high flow rate, 1.0 μl/min, a 10-fold increased in recovery of peptides compared to the conventional microdialysis probe was achieved. A probe made of a low molecular weight cutoff membrane is suitable for filtering off proteins. The new probe can be a useful tool for high recovery of peptides from living tissues. © 2010 Elsevier Inc. All rights reserved. Source

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