Ehime Institute of Industrial Technology

Imabari, Japan

Ehime Institute of Industrial Technology

Imabari, Japan

Time filter

Source Type

Tsujita T.,Ehime University | Yamada M.,Ehime University | Takaku T.,Ehime University | Shintani T.,Ehime Institute of Industrial Technology | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2011

Polyphenolic compounds from chestnut astringent skin (CAS) were purified by dialysis, using Diaion HP-20 and Sephadex LH-20 columns. During purification, specific α-amylase inhibitory activities were increased about 3.4-fold, and the 50% inhibition value was 5.71 μg/mL in the Sephadex LH-20 fraction (SE-fraction). The SE-fraction contained about 67% of the total polyphenols, 57.3% of the flavanol-type tannins, and 51.3% of the procyanidins. Strong antioxidant activity was observed in the SE-fraction. Oral administration of the SE-fraction in rats fed corn starch significantly suppressed an increase in blood glucose levels. The SE-fraction contained gallic acid and ellagic acid. The MALDI-TOF spectrum showed a peak series exhibiting a mass increment of 288 Da, reflecting the variation in the number of catechin/epicatechin units. Our results suggest CAS contains polyphenols with strong α-amylase inhibitory activity. The data also suggest CAS polyphenols might be oligomeric proanthocyanidins with gallic acid and ellagic acid. © 2011 American Chemical Society.


Wada Y.,Osaka University | Matsubara A.,Osaka University | Uchikata T.,Osaka University | Iwasaki Y.,Nagoya University | And 5 more authors.
Journal of Separation Science | Year: 2011

In this study, a high-throughput and high-sensitivity profiling system for β-cryptoxanthin (βCX) and β-cryptoxanthin fatty acid ester (βCXFA) was constructed by supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry (QqQMS). βCX and nine βCXFAs were successfully separated within 20 min using a column packed with octadecylsilyl-bonded silica particles. The limit of detection was 540 fmol for the free form and 32-130 fmol for the esterified forms. These results demonstrate that both the throughput and the sensitivity of this SFC-QqQMS system are considerably higher than those of conventional methods. When this system was applied for the analysis of Citrus unshiu, βCX and five βCXFAs were directly detected with much simpler sample pre-preparation. The analysis of other citrus fruits indicated that the βCXFA profiles varied with their breed variety. Furthermore, gas chromatography-mass spectrometry was used to analyze total fatty acid profiles in C. unshiu, and the results revealed that the profiles of fatty acids located in βCXFA were distinct. This is the first report on the analysis of βCX and its fatty acid derivatives by SFC-QqQMS. The profiling system developed in this study will be a powerful tool for investigating xanthophyll fatty acid esters. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Imamura S.,Japan National Research Institute of Fisheries Science | Suzuki M.,Japan National Research Institute of Fisheries Science | Okazaki E.,Tokyo University of Marine Science and Technology | Murata Y.,Japan National Research Institute of Fisheries Science | And 3 more authors.
Fisheries Science | Year: 2012

Thaw-rigor is often found in frozen meat of bigeye tuna Thunnus obesus. Excessive amounts of drip loss and stiffness greatly lower the commercial value of tuna meat. In order to prevent thaw-rigor in meat stored at -60°C post-capture, we adapted a temperature shift technique that stores the meat at -7°C for 1 day or -10°C for 7 days before thawing. Biochemical changes in muscle of bigeye tuna before and after the temperature shift to -7 or -10°C were characterized. Contents of ATP, NAD +, glycogen, and creatine phosphate decreased after the temperature shift. NAD + levels decreased faster than ATP levels and were highly correlated with the rigor index. Thaw-rigor occurred in muscle containing NAD + at 1 μmol/g and ATP at 7 μmol/g. On the other hand, the meat color of tuna during frozen storage changed to brown depending on the storage temperature and reflected the rate of metmyoglobin (met-Mb) formation. Met-Mb formation increase was dependent on the decrease in NADH levels during the frozen storage. A temperature shift technique with storage at -7°C for 1 day or -10°C for 7 days before thawing prevented thaw-rigor and met-Mb formation. © 2011 The Japanese Society of Fisheries Science.


Tsujita T.,Ehime University | Shintani T.,Ehime Institute of Industrial Technology | Sato H.,Japan National Institute of Advanced Industrial Science and Technology
Food Chemistry | Year: 2014

Using α-amylase inhibition as a separation guide, polyphenolic compounds from peanut seed skin were prepared. During preparation, specific α-amylase inhibitory activities were increased about 4-fold. High-resolution MALDI-TOF mass spectra showed that the structure of this sample was a series of polyflavan-3-ols, up to 15-mer, composed of catechin/epicatechin units together with several afzelechin/epiafzelechin units and gallocatechin/epigallocatechin units. The observed precious mass values suggest that the polymers consist of both interflavanoid C-C linkages (A-type) and interflavanoid ether linkages (B-type). Oral administration of the polyphenol fraction to rats fed corn starch significantly suppressed an increase in blood glucose levels in a dose dependent manner. Administration of the polyphenol fraction to rats fed maltose or sucrose delayed the increase in blood glucose levels. These results suggest peanut seed skin contains polyphenols with strong α-amylase inhibitory activity, which retard absorption of carbohydrates and mainly function through inhibition of α-amylase. © 2013 Elsevier Ltd. All rights reserved.


Tsujita T.,Ehime University | Shintani T.,Ehime Institute of Industrial Technology | Sato H.,Japan National Institute of Advanced Industrial Science and Technology
Journal of Agricultural and Food Chemistry | Year: 2013

Using α-amylase inhibition as a separation guide, polyphenolic compounds from almond (Prunus dulcis) seed skin were purified using ultrafiltration and Sephadex LH-20 and ODS columns. The purified fraction specifically and strongly inhibited α-amylase; the IC50 value was 2.2 μg/mL for pig pancreatic α-amylase. The fraction contained about 62% of the total polyphenols, 33.8% flavanol-type tannins and 30% procyanidins. Oral administration of the polyphenol fraction to rats fed corn starch significantly suppressed an increase in blood glucose levels and area under the curve (AUC), in a dose-dependent manner. High-resolution MALDI-TOF mass spectra showed that the structure of this sample is a series of polyflavan-3-ol polymers composed of catechin/epicatechin units and gallocatechin/epigallocatechin units up to 11-mer with several interflavanoid ether linkages. The results suggest almond seed skin contains highly polymerized polyphenols with strong α-amylase inhibitory activity, which retard absorption of carbohydrate. © 2013 American Chemical Society.


Fukugaichi S.,Ehime Institute of Industrial Technology | Fukugaichi S.,Ehime University | Matsue N.,Ehime University
Materials Chemistry and Physics | Year: 2015

Enhancement of the photocatalytic activity of TiO2 and its mechanism are important subjects in the field of catalysis. Under the coexistence of TiO2 with a diameter of about 20 nm, we synthesized faujasite X-type zeolite, and the product was made up of the composite of TiO2 and faujasite X-type zeolite. The composite had higher acetaldehyde photocatalytic decomposition activity than a corresponding mixture, and the activity of the composite was higher than that of TiO2 on TiO2 mass basis. The composite had higher UV light absorptivity than the corresponding mixture, and their absorption of UV light was mainly due to TiO2. The increased UV light absorptivity of the composite correlated to its increased acetaldehyde photocatalytic decomposition activity. © 2015 Elsevier B.V.


Watanabe S.,Ehime University | Hiraoka Y.,Ehime Institute of Industrial Technology | Endo S.,Ehime University | Tanimoto Y.,Ehime University | And 2 more authors.
Journal of Biotechnology | Year: 2015

Post-translational hydroxylation of the L-proline residue mainly occurs in collagen; therefore, the L-hydroxyprolines (L-Hyp) synthesized, including trans-4-hydroxy-L-proline (T4LHyp) and trans-3-hydroxy-L-proline (T3LHyp), are important markers for directly measuring the content of collagen in several biological samples. The most frequently used method to estimate the content of L-Hyp is high-performance liquid chromatography (HPLC), which is inconvenient. In the present study, we attempted to estimate the content of L-Hyp using coupling systems with metabolic enzymes of the T4LHyp (hydroxyproline 2-epimerase (HypE) and cis-4-hydroxy-D-proline dehydrogenase (HypDH)) and T3LHyp pathways (T3LHyp dehydratase (T3LHypD) and δ1-pyrroline-2-carboxylate reductase (Pyr2CR)) from microorganisms. We constructed a functional expression system of recombinant HypDH with a heterooligomeric structure in Escherichia coli cells. Enzymological characterization revealed that the β-subunit acted as a catalytic subunit, and also that assembly with other subunit(s) improved the kinetics for cis-4-hydroxy-D-proline and thermostability. By using a spectrophotometric assay with different wavelengths, the contents of T4LHyp and T3LHyp were successfully estimated within the ranges of 0.004-1mM and 0.05-1mM, respectively, and were consistent with those determined by HPLC. This enzymatic method was used to measure the content of T4LHyp in the acid-hydrolysate of collagen, and blood plasma. © 2015 Elsevier B.V.


Nakamura K.,Ehime Institute of Industrial Technology | Takao M.,Chiyoda Corporation
Sen'i Gakkaishi | Year: 2013

Various saccharification and fermentation conditions were examined to produce bioethanol efficiently from cotton. These conditions indicate liquor-to-sample ratios, amount of cellulase addition, amount of acetic acid buffer solution and the pH of acetic acid buffer solution. These conditions were varied within the range of practical conditions which can be achieved for a dyeing equipment. Furthermore, we adopted room temperature and short time alkali soaking as a pretreatment method. This method also can be achieved for a dyeing equipment. As a result of examinations, we got an optimized condition that was pH 5.0, 20% of cellulase addition for cotton's weight and we also cleared the relationship between liquor-to-sample ratios and obtained glucose concentration through a saccharification. The efficiency of ethanol fermentation was found to be 66-75% of theoretical yield.


Fukugaichi S.,Ehime Institute of Industrial Technology | Henmi T.,Ehime University | Matsue N.,Ehime University
Catalysis Letters | Year: 2013

TiO2-zeolite composite was prepared through synthesis of X-type zeolite in the presence of anatase type TiO2. The composite had higher acetaldehyde photocatalytic activity than the mixture of the two. In the composite, TiO2 particle was closely attached to zeolite crystal, and the attachment might support the transfer of acetaldehyde molecules from zeolite to TiO2. Graphical Abstract: [Figure not available: see fulltext.] © 2013 Springer Science+Business Media New York.


PubMed | Ehime University and Ehime Institute of Industrial Technology
Type: | Journal: Journal of biotechnology | Year: 2015

Post-translational hydroxylation of the L-proline residue mainly occurs in collagen; therefore, the L-hydroxyprolines (L-Hyp) synthesized, including trans-4-hydroxy-L-proline (T4LHyp) and trans-3-hydroxy-L-proline (T3LHyp), are important markers for directly measuring the content of collagen in several biological samples. The most frequently used method to estimate the content of L-Hyp is high-performance liquid chromatography (HPLC), which is inconvenient. In the present study, we attempted to estimate the content of L-Hyp using coupling systems with metabolic enzymes of the T4LHyp (hydroxyproline 2-epimerase (HypE) and cis-4-hydroxy-D-proline dehydrogenase (HypDH)) and T3LHyp pathways (T3LHyp dehydratase (T3LHypD) and (1)-pyrroline-2-carboxylate reductase (Pyr2CR)) from microorganisms. We constructed a functional expression system of recombinant HypDH with a heterooligomeric structure in Escherichia coli cells. Enzymological characterization revealed that the -subunit acted as a catalytic subunit, and also that assembly with other subunit(s) improved the kinetics for cis-4-hydroxy-D-proline and thermostability. By using a spectrophotometric assay with different wavelengths, the contents of T4LHyp and T3LHyp were successfully estimated within the ranges of 0.004-1mM and 0.05-1mM, respectively, and were consistent with those determined by HPLC. This enzymatic method was used to measure the content of T4LHyp in the acid-hydrolysate of collagen, and blood plasma.

Loading Ehime Institute of Industrial Technology collaborators
Loading Ehime Institute of Industrial Technology collaborators