Egyptian Plant Protection Res Institute

Al Jizah, Egypt

Egyptian Plant Protection Res Institute

Al Jizah, Egypt
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Extract of the plant Costus speciosus was evaluated for its potential acaricidal and ovicidal activities against the two-spotted spider mite, Tetranychus urticae Koch, for the first time under laboratory conditions. The acaricidal activity was performed by studying the mortality, fecundity, fertility and offspring development of adult females at two different temperatures. The LC50 value of C. speciosus is 2.3 %. Treatment significantly affected the fecundity of the females, percentage of hatched eggs, percentage of eggs reaching adult stages and duration of each life cycle stage. The acaricidal efficacy of this extract is temperature dependent. The greater susceptibility of T. urticae to C. speciosus was recorded at a lower temperature. Concerning the ovicidal activity of C. speciosus, extract exhibited 79.67 ± 4.01 % mortality on eggs. It also significantly delays the egg hatching of T. urticae compared to the control. Results showed that hatching started on the sixth day post-deposition in the treated group compared to the fourth day in the control group. All hatched eggs turned into nymphs in the control group while treatment reduced the number of hatched eggs and caused death of all hatched larvae before reaching nymphal stages. Extract of C. speciosus has promising acaricidal and ovicidal activities that could lead to the development of new bio-acaricides and is worth further investigation. © 2016 Entomological Society of Southern Africa.


Moustafa H.Z.,Egyptian Plant Protection Res Institute
Egyptian Journal of Biological Pest Control | Year: 2016

Toxicity, biological and biochemical effects of methanol extracts of two plants extracts namely, Conyza dioscoridis and Melia azedarach against the newly hatched larvae of the pink bollworm, Pectinophora gossypiella (Saunds.) and the spiny bollworm, Earias insulana (Boisd.) under laboratory conditions were investigate. The results showed susceptibility of P. gossypiella larvae towards the plant extract of C. dioscoridis was much higher than that of E. insulana larvae; whereas E. insulana larvae was more susceptible to the toxicity of M. azedarach than P. gossypiella larvae after 2, 5 and 7-days post treatments. It was obvious that the toxicity of the two methanol extracts increased as periods of post treatments increased. Treatment of newly hatched larvae with LC50 values with the two plant extracts caused alteration in the biochemical aspects of the resulted 4th instar larvae. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved.


Nouh G.M.,Egyptian Plant Protection Res Institute
Egyptian Journal of Biological Pest Control | Year: 2016

Pathogenicity and persistence of two species of the entomopathogenic nematodes (EPN) Heterorabditis bacteriophora and Steinernema abbasi against last instar larvae of the greater wax moth Galleria mellonella L. was evaluated. In addition, host finding ability, survival of nematodes stored in sandy soil and production of infective juveniles (IJs) of both species were studied. Mortality percentages increased as the concentration of (IJs) has increased and height of sandy soil column has decreased. Mortality increased also as the time of exposure increased to one week. H. bacteriophora was generally more virulent than S. abbasi. H. bacteriophora was more persistent against G. mellonella larvae as it caused 100% mortality in all concentrations and all storage periods. Meanwhile, S. abbasi had caused 100, 60 and 52% mortalities at the concentrations of 70, 140 and 280 IJs/cm2 of soil surface after three weeks of storage. Production of H. bacteriophora from G. mellonella larva was 55400 IJs, at the lowest concentration, 5 IJs/larva. The production rate increased to 229660 IJs/larva at the highest concentration of 200/larvae. G. mellonella larvae treated with S. abbasi produced 30080 IJs /larva, at the lowest concentration, 5 IJs/larva. Production rate increased to 177800 IJs/ larva at the highest concentration of 200 IJs/ larva. © 2016 Egyptian Society for Biological Control of Pests. All rights reserved.


Ali S.S.,Egyptian Plant Protection Res Institute
Egyptian Journal of Biological Pest Control | Year: 2016

Three forms of laboratory formulations of the entomopathogenic isolated fungus Beauveria bassiana were prepared as Wettable-Powder (WP), Dust (D) and Emulsion (E). The formulations were evaluated by two experiments: effect of storage duration on spores’ viability testing the three fungus formulations, and virulence of B. bassiana tested formulations against larvae of the cotton leaf worm, Spodoptera littoralis (Boisd.). Dust formulation was the best formulation for maintaining the viability of conidia longer and then wettable powder. On the contrary, the emulsion was the lowest for keeping the spores survived throughout the tes ted periods of storage. In the second evaluation, dust formulation was more virulent to S. littoralis larvae than the other formulations. In addition, WP and E formulations were less virulent when compared to pure conidia (control) or D formulation. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved.


Afsah A.F.E.,Egyptian Plant Protection Res Institute
Annals of Agricultural Sciences | Year: 2015

All experiments were conducted at the Experimental Farm of Plant Protection Research Station at Qaha, Qalubia Governorate, Egypt, throughout two successive growing seasons (2012 and 2013) on vegetative period. The results clearly indicated the presence of 36 species belonging to 25 families under 10 orders. These insects and mites are as follows: Insect species were clover leaf weevil, Phytonomus brunneipennis Boh.; clover root weevil, Sitona Lividipes Fahraeus; cutworm, Agrotis ipsilon (Huf.); green bug, Nezara viridula L.; Semillopers, Autographa gamma L.; tomato borer, Tuta absoluta Povolny; milkweed bug, Spilostethus pandurus Scop; white-spotted bug, Eysarcoris ventralis (Westwood); mealybug, Planococcus citri Risso; red bug, Scantius aegyptius (L.); cotton whitefly, Bemisia tabaci (Genn.); pea aphid, Acyrthosiphon pisum (Harris); cotton aphid, Aphis gossypii Glover; potato aphid, Macrosiphum euphorbiae (Thomas); green peach aphid, Myzus persicae (Sulzer); cotton jassids, Empoasca lybica (de Berg); cotton thrips. Thrips Tabaci Lind; cotton seed bug, Oxycarenus hyalinipennis Costa; adonis beetle, Hippodamia (Adonia) variegate (Goeze); Ladybird beetles, Coccinella spp. Linnaeus; Flower bug, Orius spp. Say; Aphid lion, Chrysoperla carnea (Stephens); mantis, Mantis religiosa L.; Gosmlyce baeticus L.; cabbage worm, Pieris rapae (Linnaeus); spiny bollworm, Earias insulana (Boisduval) and colorful butterfly, Vanessa cardui (Linnaeus). Mite species were the red spider mite, Tetranychus spp.; eriophyid mite (tomato russet mite), Aceria lycopersici (Wolffenstein); broad mite, Polyphagotarsonemus latus (Banks); Phytoseiulus persimilis Athias-Henriot, Amblyseius gossypii EL-Badery, Stigmaeus rattus Gomaa and Rakha, Cunaxa spp.; the red spider mite, Tarsonemus spp. and Tydeus spp. The results showed that Aceria lycopersici proved to be the most abundant species followed by Bemisia tabaci and the Tetranychus spp. recorded with occurrence percent 87.15, 4.63 and 4.16% from grand mean total, respectively. Data showed that Solfan, KZ oil and Achook have suppressed effect on the individuals activity of the eriophyid mites, red spider mite and whitefly compared with control. © 2015


Simmons A.M.,U.S. Department of Agriculture | Shaaban A.-R.,Egyptian Plant Protection Res Institute
Pest Management Science | Year: 2011

BACKGROUND: The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is an important pest of vegetables and many other crops worldwide. Eight biorational insecticides (based on oil, plant derivatives, insect growth regulator and fungus) were evaluated in the field for their influence on populations of six natural enemies of B. tabaci. Natural populations of two predators [Chrysoperla carnea Stephen (Neuroptera: Chrysopidae) and Orius spp. (Hemiptera: Anthocoridae)] and two genera of parasitoids [Encarsia spp. and Eretmocerus spp. (Hymenoptera: Aphelinidae)] were evaluated in eggplant (Solanum melongena L.). Also, augmented field populations of three predators [C. carnea, Coccinella undecimpunctata L. (Coleoptera: Coccinellidae) and Macrolophus caliginosus (Wagner) (Hemiptera: Miridae)] were evaluated in cabbage (Brassica oleracea var. capitata L.), cucumber (Cucumis sativus L.) and squash (Cucurbita pepo L.). RESULTS:Regardless of natural enemy or crop, jojoba oil, Biovar and Neemix had the least effect on abundance of the natural enemies in comparison with the other insecticides during a 14 day evaluation period. Conversely, Admiral, KZ oil, Mesrona oil, Mesrona oil + sulfur and natural oil had a high detrimental effect on abundance of the natural enemies. CONCLUSION: These results demonstrate the differential effects of biorational insecticides for whitefly control on predators and parasitoids in the field. Published 2011 by John Wiley & Sons, Ltd. This article is a US Government work and is in the public domain in the USA. Published 2011 by John Wiley & Sons, Ltd..


El-Gendy I.R.,Egyptian Plant Protection Res Institute
International Journal of Agricultural Research | Year: 2012

The Peach Fruit Fly (PFF), Bactrocera zonata (Saunders) (Diptera: Tephritidae) is an important agricultural pest in Egypt. Monitoring and control of this species is relating to collecting data of traps catching. The efficiency of traps attraction has an important role to reach the real population density of PFF. Present study was carried out in the field on Jackson trap provided with the sex attractant, Methyl eugenol, to evaluate the effects of color and height of the trap as well as position of the trap in the main cardinal directions of the tree on attraction of male PFF, expressed as male flies captured per trap per day (CTD). Results of the comparison between yellow and white colors of card Jackson traps revealed that white trap color (4.67 CTD) was preferred than the yellow one (3.63 CTD) for peach fruit fly attraction. The suitable height for hanging traps on the tree was 1.5 m, followed by 1.0, 2.5 and 2.0 m heights, respectively. On the other hand, the best position of trap on the tree was West direction (3.33 CTD) of the tree, followed by North (3.20 CTD), East (1.90 CTD) and South directions (1.60 CTD), respectively. © 2012 Academic Journals Inc.


El-Sayed A.A.A.,Egyptian Plant Protection Res Institute
Journal of Entomology | Year: 2016

The effect of LC20 of hexaflumuron on some biological aspects, egg maturation and ovary of the spiny bollworm,Earias insulana (Boisd.) were studied under controlled conditions of 26±1°C and 75±5 RH. The calculated LC20 and LC50 values of hexaflumuron were 17.42 and 72.75 ppm. The treatment newly hatched larvae with LC20(17.42 ppm) elongated larval and pupal duration compared with control. Treatment significantly reduced mean numbers of deposited eggs and hatchability percentage compared with control. Hexaflumuron reduced number of immature eggs after emergence (20.56 eggs/ovariol) compared with 27.05 eggs/ovariol for untreated. No mature eggs were present in the female moths ovarioles after emergence of treated and untreated. Mature eggs were found after 36 h and 3 days in untreated and treated, respectively. The highest numbers of mature eggs 3.63 and 6.32 eggs/ovariol were recorded after four days from emergence in treated and untreated, respectively. Hexaflumuron reduced the ovarian weight and length, size of basal eggs and number of spermatophore/female compared with untreated. The LC20 of hexaflumuron reduced significantly larval content of total carbohydrate, lipid and soluble protein. © 2016 A.A.A. El-Sayed.


El-Zahi E.-Z.S.,Egyptian Plant Protection Res Institute
Egyptian Journal of Biological Pest Control | Year: 2012

Profenofos and six novel pesticides viz., pyridalyl, spinosad, indoxacarb, emamectin-benzoate, thiamethoxam and abamectin were evaluated against green lacewing, Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) through laboratory bioassay. C. carnea eggs were immersed in pesticides solutions, while second instar larvae were exposed using leaf dipping and thin film techniques. Recommended dose of each pesticide was estimated. Latent effects on consequent stages, percent pupation and adult emergence, were studied as well. Spinosad and indoxacarb were non-toxic causing 0.0% mortality to 24 h old eggs. Pyridalyl, abamectin and emamectin-benzoate were harmless resulting in less than 10% egg mortality, while profenofos was highly toxic causing 86.97% mortality. 48 h old eggs were more susceptible to all tested toxicants than 24 h old eggs. Based on the classification given by IOBC/WPRS, all tested novel pesticides were harmless to C. carnea larvae and pyridalyl and abamectin were the most harmless causing less than 10% mortality, whereas profenofos was harmful recording 100% larval mortality. With exception of pyridalyl, the tested pesticides were more toxic to C. carnea larvae using thin film technique comparing to leaf dipping technique. The six novel pesticides exhibited latent effects to treated larvae on subsequent stages causing 44.45 - 80.00% pupation and 37.50 - 75.00% adult emergence. Further field studies should be conducted to confirm the harmlessness of novel pesticides on beneficial arthropods, particularly under field conditions.


El-Gendy I.R.,Egyptian Plant Protection Res Institute
International Journal of Agricultural Research | Year: 2012

Mediterranean Fruit Fly (MFF), Ceratitis capitata and Peach Fruit Fly (PFF), Bactrocera zonata are the main insect pests in Egypt and because the monitoring process is done by sex attractant traps that is specialize for males only, while food baits extend to attract both male and female flies and thus, it can be used in monitoring fruit flies, especially female flies. Responding both MFF and PFF to food baits in field trials were studied as aim to evaluate the attraction to some protein derivatives; Cera Trap, Buminal and Bio Nal, in comparison with torula yeast, also effect of pH degree. Results indicated that the highest attractive material for MFF was Buminal bait, followed by Cera Trap and Bio Nal. While, the highest attractive material for PFF was Cera Trap bait, followed by Buminal and Bio Nal. Significant differences in attraction were obtained among different food baits, also between females and males attraction for both MFF and PFF. Number of captured flies for both species was related to pH degree. A significant negative correlation was obtained between concentrations of different food baits in pH degree. A significant negative correlation was obtained for Buminal baits in pH degree with elapsed time while non-significant negative and non-significant positive correlation were obtained for Cera Trap and Bio Nal baits in pH degree with elapsed time. © 2012 Academic Journals Inc.

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