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Simon A.S.,Pushpagiri Institute of Medical science | Chithra V.,Pushpagiri Institute of Medical science | Vijayan A.,Pushpagiri Institute of Medical science | Dinesh R.D.,Center for Advanced Genetic Studies | Vijayakumar T.,Educare Institute of Dental science
Journal of Biosciences

Coronary artery disease (CAD) is a multifactorial disease caused by the interplay of environmental risk factors with multiple predisposing genes. The present study was undertaken to evaluate the role of DNA repair efficiency and oxidative stress and antioxidant status in CAD patients. Malonaldehyde (MDA), which is an indicator of oxidative stress, and mean break per cell (b/c) values, which is an indicator of decreased DNA repair efficiency, were found to be significantly increased in patients compared to normal controls (P < 0.05) whereas ascorbic acid and GSH were found to be lower among patients than the control group. It has been found that elevated oxidative stress decreased antioxidant level and decreased DNA repair efficiency can contribute to the development of CAD. This study also showed that high MDA, low ascorbic acid and GSH were significantly associated with high b/c value. © 2013 Indian Academy of Sciences. Source

Simon A.S.,Pushpagiri Institute of Medical science and Research Center | Vijayakumar T.,Educare Institute of Dental science
Indian Journal of Clinical Biochemistry

Coronary artery disease (CAD) remains the major cause of mortality and morbidity in the entire world population. The conventional risk factors of CAD include hypertension, hyperlipidemia, diabetes mellitus, family history, smoking etc. These factors contribute only 50 % of the total risk of CAD. For providing a complete risk assessment in CAD, it is mandatory to have well-planned clinical, biochemical and genetic studies in patients with CAD and subjects who are at risk of developing CAD. In this review an attempt is made to critically evaluate the conventional and emerging risk factors which predispose the individual to CAD. Specifically, the molecular basis of CAD including high oxidative stress, low antioxidant status and increased DNA damage are covered. A comprehensive and multifactorial approach to the problem is the better way to reduce the morbidity and mortality of the disease. © 2013 Association of Clinical Biochemists of India. Source

Rammanohar M.,Educare Institute of Dental science | Raju Ragavendra T.,Peoples Dental Academy
Journal of Forensic Sciences

Dental hard tissues are good candidates for age estimation as they are less destructive and procedures to determine age can be easily performed. Although cementum annulations and cementum thickness are important parameters in this regard, they are seldom used. This study was undertaken to review the methods, difficulties in execution of techniques, and accuracy of cementum thickness and annulations in estimating the age. Unstained and stained ground sections of tooth were used to measure cemental thickness and count cemental annulations based on which age was estimated and was compared with known age. Although there was positive relation between cemental thickness and annulations with age, only in 1-1.5% of cases, age could be predicted with accuracy. © 2010 American Academy of Forensic Sciences. Source

Kumar K.,P.A. College | Manohar M.R.,Educare Institute of Dental science
Analytical and Quantitative Cytology and Histology

OBJECTIVE: To determine the effect of formalin fixation time on histomorphometry by changing the fixation time and the effect of the age of processing solutions on histomorphometry by processing tissues in solutions of varying ages. STUDY DESIGN: The study consisted of two parts. The first part examined the effect of formalin fixation time on histomorphometry by changing the fixation time. The second part looked at the the effect of the age of processing solutions on histomorphometry by processing tissues in solutions of different ages. RESULTS: In first part of the study, cytoplasmic expansion was observed between 24 hours and 1 week of fixation, followed by shrinkage thereafter. The nuclear area showed progressive shrinkage from 24 hours to 1 month. In second part of the study, tissues processed in fresh solution showed more shrinkage than tissues processed in used solution. CONCLUSION: It is important to be aware of changes in histomorphometric values resulting from a variable fixation period and age of processing solutions. It is also important to standardize the histomorphometric procedure or take into account the effect of factors that influence morphometric values to minimize the errors due to the same. © Science Printers and Publishers, Inc. Source

Divya K.S.,Educare Institute of Dental science
Indian journal of dental research : official publication of Indian Society for Dental Research

Langerhans cell histiocytosis (LCH) is a rare idiopathic disease characterized by the clonal proliferation of Langerhans cells. LCH affects five children per million population. The peak incidence is from 1 to 4 years of age. LCH involves the head and neck region quite commonly. Oral soft tissue lesions are also common. The differential diagnosis of oral LCH includes leukemia, neutropenia, prepubertal periodontitis, hypophosphatasia, fibrous dysplasia, and Papillon-Lefevre syndrome. The prognosis of LCH depends on early detection and appropriate management. Surgical management alone is used in 50% of cases with an additional 23% of the lesions being treated with both surgery and radiation therapy. A case of LCH in a 6-year-old girl involving the mid root level of developing first permanent molar with a floating developing tooth bud of permanent second molar mimicking an inflammation is reported. Source

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