Ecole Nationale Superieure Veterinaire

Algeria

Ecole Nationale Superieure Veterinaire

Algeria
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Akkou M.,Ecole Nationale Superieure Veterinaire | Akkou M.,Blida University | Antri K.,University of Science and Technology Houari Boumediene | Bachtarzi M.-A.,Mustapha University Hospital Center | And 9 more authors.
Pakistan Veterinary Journal | Year: 2016

Staphylococcus aureus is a major bovine mastitis pathogen responsible for heavy economic losses in dairy industry. Identification of epidemiological aspects associated with bovine mastitis may be helpful in treatment and management decisions. Due to high concern of zoonotic infections, we describe here, the distribution and antimicrobial susceptibility patterns of S. aureus from both cows with mastitis and nasal carriage of workers in contact to animals. Up to 38% workers were nasal carriers. Besides, S. aureus was isolated among 74% dairy herds suffering from mastitis, within 29.8% of mastitic quarter-milk samples. The isolates were tested for antimicrobial resistance, gyr, mecA, mecC and agr alleles. The gene gyr was detected in all S. aureus strains, 91 (77.7%) belonged to agr specificity group I, 11.9% affiliated to group II, 10 (8.5%) were agrIII, and 1.7% human derived isolates to the group IV. agr I was dominant in both human and animal isolates with 60 and 91%, respectively. Four human isolates harbored mecA gene, while no mecA or mecC genes were found in bovine derived isolates. Overall, 92% human isolates and 86.5% of cows' derived strains were resistant to penicillin G. The resistance against non beta-lactam antibiotics was considerably greater in human than cows' derived isolates, while different patterns of resistance share the same ecological niches. The high association of penicillin resistance to S. aureus in bovine mastitis highlights periodic surveillance of antibiotic resistance in livestock. © 2015 PVJ.


Ait-Oudhia K.,Ecole Nationale Superieure Veterinaire | Ait-Oudhia K.,Montpellier University Hospital Center | Gazanion E.,Montpellier University | Sereno D.,Montpellier University | And 5 more authors.
Veterinary Parasitology | Year: 2012

The aim of this study was to evaluate the susceptibility to anti-leishmanial agents of 24 strains isolated from dogs living in the urban area of Alger lacking drug selection pressure. Two different Leishmania infantum zymodemes, MON-1 and MON-281, were identified in these dogs. The in vitro susceptibility to the main forms of antimonial and amphotericin were assessed on promastigote and amastigote life stages in culture. The results obtained for both parasite life stages were concordant whatever the molecule tested. Moreover, our data showed that isolates belonging to the relatively rare zymodeme of L. infantum, MON-281, were less susceptible to antimony than MON-1, when at the same time there was no significant difference for amphotericin B. © 2012 Elsevier B.V.


Baroudi D.,Ecole Nationale Superieure Veterinaire | Baroudi D.,Centers for Disease Control and Prevention | Khelef D.,Ecole Nationale Superieure Veterinaire | Hakem A.,University Ziane Achor | And 6 more authors.
Veterinary Parasitology: Regional Studies and Reports | Year: 2017

Little is known on the identity and public health potential of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in farm animals in Algeria. In this study, 102 fecal specimens from pre-weaned dairy calves with or without diarrhea were collected from 19 dairy farms located in 6 provinces. PCR-restriction fragment length polymorphism analysis of the small subunit rRNA gene was used to detect and differentiate Cryptosporidium spp., whereas PCR-sequence analysis of the triosephosphate isomerase gene and ribosomal internal transcribed spacer were used to detect and genotype G. duodenalis and E. bieneusi, respectively. Cryptosporidium was found in 14 specimens, among which 7 had C. parvum, 4 had C. bovis, and 3 had mixed infection of C. parvum and C. bovis or C. bovis and C. andersoni. Subtyping of C. parvum by PCR-sequence analysis of the 60 kDa glycoprotein gene identified two zoonotic subtypes IIaA16G2R1 and IIaA17G3R1. G. duodenalis was found in 28 specimens, with 6 having the host-specific assemblage E, 14 having the zoonotic assemblage A (all belonging to A2 subtype), and 8 having mixed assemblages. Six known genotypes of E. bieneusi belonging to Group 2, including I, J, BEB3, BEB4, BEB6 and PtEb XI, were identified in 11 specimens. Diarrhea was mostly associated with the occurrence of C. parvum. Data from this study suggest that human-pathogenic C. parvum subtypes and G. duodenalis and E. bieneusi genotypes are common on dairy farms in Algeria. © 2017


Bessas A.,Ecole Nationale Superieure Veterinaire | Bessas A.,University of Laghouat | Leulmi H.,Ecole Nationale Superieure Veterinaire | Leulmi H.,Aix - Marseille University | And 7 more authors.
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2016

In Algeria, only limited information is currently available on the prevalence of emergent canine and feline vector-borne diseases. The aim of the present work was to detect by qPCR vector-associated bacteria in stray dogs and cats and their ectoparasites from Algiers.18/117 (15.38%) dogs and 2/107 (1.87%) cats were positive for at least one vector-borne agent. Coxiella burnetii and Bartonella henselae were identified in 1/117 (0.85%) dog individually. Ehrlichia canis DNA was detected in 17/117 (14.52%) dogs. 1/107 (0.93%) cat was positive to C. burnetii and another 1/107 (0.93%) to B. henselae. DNA of Rickettsia massiliae, Rickettsia conorii and E. canis was detected in Rhipicephalus sanguineus. Cat fleas were infected with Rickettsia felis, B. henselae and Bartonella clarridgeiae. B. vinsonii subsp. berkhoffii was identified in Xenopsylla cheopis collected from dogs. The findings of this study indicate that dogs and cats from Algeria are exposed to multiple tick and flea-borne pathogens. © 2016 Elsevier Ltd.


Ghozlane M.K.,Ecole Nationale Superieure Veterinaire | Bouamra M.,Ecole Nationale Superieure Veterinaire | Temim S.,Ecole Nationale Superieure Veterinaire
Livestock Research for Rural Development | Year: 2012

This study has been realized in the ruminant station of technical institute of Baba-Ali, Algiers. On a period of seven months, between December 2010 and June 2011. It focused on the eating behavior of 41 Prim'Holstein dairy cows, two months before calving and during the first five months of lactation, and its impact on the change in body condition score. The nutrient intakes of nine different diets were measured from the estimated amounts of dry matter ingested during our test, and nutritional value of foods calculated using the results of feed analysis. In parallel, the herd status has been appreciated through a monthly assessment of body condition score. The latter was estimated on average to 3.79 ± 0.50 at calving and 60 days after parturition to 3.04 ± 0.45, a loss of 0.75 points over the first two months of lactation. The study of correlations revealed that the impact of the feeding on the body condition score was delayed in time, and it was especially important at calving. Moreover, it appears that the proportion of concentrate in the diet during this period increases the degree of mobilization of body reserves. This work, however, allowed to bring over a report concerning the influence of feeding management in the peri and postpartum on the body condition score variation.


Saidi R.,Blida University | Khelef D.,Ecole Nationale Superieure Veterinaire | Kaidi R.,Blida University
Livestock Research for Rural Development | Year: 2012

Artificial insemination (AI) is considered one aspect of biotechnology in animal reproduction most commonly practiced throughout the world. To evaluate the results of this biotechnology that has been practiced for years in cattle in Algeria, a set criteria of reproduction was analyzed, including: days from calving to calving (DC-C), The delay between calving and first insemination of cows (DC-AI1), days from calving to conception, the rate of conception at first insemination (RCAI1), the percentage of females fertilized after 3 or more AI and index coital. The study was conducted from 2002-2008 in five consecutive years. In light of the results obtained, it appears that: DC-C is 461 ± 16.1 days (d), DC-1 STAI is106 ± 85.4 d, days from calving to conception are185 ± 137 d, the RCAI1 is 41.1% and the percentage of females requiring 3 AI or more is 39.3%. Too bad percentage is added an index coital of 2.47. These poor results of AI are the consequence in part to the lack of control by stakeholders in the livestock breeding management, on other part linked to the mismatch between food intake and physiological state of the female.


Miroud K.,University of El-Tarf | Hadef A.,University Of Constantine1 | Khelef D.,Ecole Nationale Superieure Veterinaire | Ismail S.,Blida University | Kaidi R.,Blida University
Livestock Research for Rural Development | Year: 2014

The aim of this study was to establish a reproduction balance sheet so as to quantify reproductive performances and particularly the duration of postpartum anoestrus via the follow up of 40 dairy herds located in north-east Algeria. The results obtained showed that the mean calving-1st heat interval, the mean calving-1st insemination interval, the mean 1st insemination-conception interval, the mean conception interval and the mean calving interval were significantly higher than the reference values (71.4 ± 32.6 days vs <50 days; 72.3 ± 24.8 days vs 60-70 days; 69 ± 50.4 daysvs 23-30 days; 148 ± 96.2 days vs 85 days; 430 ± 75 days vs 365 days respectively), that the first insemination conception rate was very low (25%) and that 43.5% of cows were inseminated more than twice. Moreover, the Herd Reproductive Status of all dairy herds involved was too low and sometimes even negative which revealed that fertility and fecundity were greatly affected. A significant difference between the 8 large (≥ 50 dairy cows) and the 32 small (< 50 dairy cows) dairy farms has only concerned the calving-1st heat interval and the conception interval (p < 0.05).


PubMed | Aix - Marseille University, Ecole Nationale Superieure Veterinaire, University of Laghouat and French Institute of Health and Medical Research
Type: | Journal: Comparative immunology, microbiology and infectious diseases | Year: 2016

In Algeria, only limited information is currently available on the prevalence of emergent canine and feline vector-borne diseases. The aim of the present work was to detect by qPCR vector-associated bacteria in stray dogs and cats and their ectoparasites from Algiers. 18/117 (15.38%) dogs and 2/107 (1.87%) cats were positive for at least one vector-borne agent. Coxiella burnetii and Bartonella henselae were identified in 1/117 (0.85%) dog individually. Ehrlichia canis DNA was detected in 17/117 (14.52%) dogs. 1/107 (0.93%) cat was positive to C. burnetii and another 1/107 (0.93%) to B. henselae. DNA of Rickettsia massiliae, Rickettsia conorii and E. canis was detected in Rhipicephalus sanguineus. Cat fleas were infected with Rickettsia felis, B. henselae and Bartonella clarridgeiae. B. vinsonii subsp. berkhoffii was identified in Xenopsylla cheopis collected from dogs. The findings of this study indicate that dogs and cats from Algeria are exposed to multiple tick and flea-borne pathogens.

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