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Wyndmoor, PA, United States

Zhou X.,Cornell University | Cooke P.,Eastern Regional Research Center | Li L.,Cornell University
Journal of Experimental Botany | Year: 2010

Germination and early seedling development are coordinately regulated by glucose and phytohormones such as ABA, GA, and ethylene. However, the molecules that affect plant responses to glucose and phytohormones remain to be fully elucidated. Eukaryotic release factor 1 (eRF1) is responsible for the recognition of the stop codons in mRNAs during protein synthesis. Accumulating evidence indicates that eRF1 functions in other processes in addition to translation termination. The physiological role of eRF1-2, a member of the eRF1 family, in Arabidopsis was examined here. The eRF1-2 gene was found to be specifically induced by glucose. Arabidopsis plants overexpressing eRF1-2 were hypersensitive to glucose during germination and early seedling development. Such hypersensitivity to glucose was accompanied by a dramatic reduction of the expression of glucose-regulated genes, chlorophyll a/b binding protein and plastocyanin. The hypersensitive response was not due to the enhanced accumulation of ABA. In addition, the eRF1-2 overexpressing plants showed increased sensitivity to paclobutrazol, an inhibitor of GA biosynthesis, and exogenous GA restored their normal growth. By contrast, the loss-of-function erf1-2 mutant exhibited resistance to paclobutrazol, suggesting that eRF1-2 may exert a negative effect on the GA signalling pathway. Collectively, these data provide evidence in support of a novel role of eRF1-2 in affecting glucose and phytohormone responses in modulating plant growth and development.

News Article
Site: phys.org

Now, a team of Agricultural Research Service scientists in Wyndmoor, Pennsylvania, has made key advances in a process that produces a crude liquid called "bio-oil" from agricultural waste. The crude bio-oil is produced by pyrolysis, a process that involves chemical decomposition of plant and other organic matter using very high heat. The modified technique is called "tail-gas reactive pyrolysis" (TGRP). It holds promise for processing and improving the bio-oil, which is ultimately processed into finished biofuel. The Energy Independence and Security Act of 2007 calls for a minimum of 36 billion gallons of advanced biofuels to be produced in the U.S. by the year 2022. This effort will require, in part, the development of a new industry that produces 21 billion gallons of new biofuels based on non-food sources. "Ideally, the biofuels added to gasoline would be identical to fuels produced at petroleum refineries," says chemical engineer Yaseen Elkasabi. The research team, which includes Elkasabi, is headed by chemical engineer Akwasi Boateng with chemist Charles Mullen, and engineers Neil Goldberg and Mark Schaffer, in the Sustainable Biofuels and Coproducts Unit at the ARS Eastern Regional Research Center. Raw material called "biomass" is the basis for producing biofuel, and it includes non-food-grade plant matter procured from agricultural or household waste. "We are using crop and forestry residue, such as wood and switchgrass, and also animal manures to produce bio-oils at an accelerated rate using a new high-output, mobile processing unit," says Mullen. "Rather than shipping large amounts of agricultural waste to a refinery plant at a cost, the mobile reactor allows us to convert the biomass into a more energy-dense bio-oil right on the farm." The goal of using TGRP on the farm is to yield a higher quality bio-oil that is more marketable to biofuel producers than bio-oil made from traditional pyrolysis methods. Construction of the mobile unit was funded by a Biomass Research and Development Initiative grant from USDA's National Institute of Food and Agriculture. At petroleum refineries, distillation is a process used for preparing crude oils into finished fuels. But traditional petroleum refineries are not equipped to distill crude pyrolysis oil because it is highly acidic and has high oxygen content, making it corrosive and thermally unstable. Petroleum is naturally deoxygenated. While crude bio-oil can be deoxygenated by adding a catalyst, that approach is expensive and complex. The ARS team's studies have shown that the new TGRP process provides bio-oils that are similar in composition and properties to those produced by adding the catalyst. "The quality of TGRP deoxygenated liquids is equal to or better than the bio-oil produced by catalytic pyrolysis," says Elkasabi. "TGRP is an important step toward the ultimate goal of producing cleaner bio-oils that can be distilled at existing petroleum refineries."

Schneider M.J.,Eastern Regional Research Center | Mastovska K.,Eastern Regional Research Center | Solomon M.B.,Beltsville Area Research Center
Journal of Agricultural and Food Chemistry | Year: 2010

The U.S. Food and Drug Administration sets tolerances for veterinary drug residues in muscle but does not specify which type of muscle should be analyzed. To determine if antibiotic residue levels are dependent upon muscle type, seven culled dairy cows were dosed with penicillin G (Pen G) from 1 to 3 days and then sacrificed on day 1, 2, or 5 of withdrawal. A variety (9-15) of muscle samples were collected, along with liver and kidney samples. In addition, corresponding muscle juice samples were prepared. All samples were extracted and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) to determine Pen G levels. Results showed that Pen G residue levels can vary between and within different muscles, although no reproducible pattern was identified between cows or withdrawal times. Muscle juice appeared to be a promising substitute for muscle as a matrix for screening purposes. Because of the potential for variation within muscles, all samples taken need to be large enough to be representative. © This article not subject to U.S. Copyright. Published 2010 by the American Chemical Society.

The shrub willow plantation is part of a broader five-year program called NEWBio, which is aimed at investigating and promoting sustainable production of woody biomass and warmseason grasses for energy in the Northeast. Planted in 2012 on land formerly owned by the State Correctional Institution at Rockview, the biomass crop will regrow and will be harvested every three years from now on. NEWBio, a regional consortium of institutions lead by Penn State and funded by the U.S. Department of Agriculture's National Institute of Food and Agriculture, is one of seven regional projects across the United States. Other consortium partners are Cornell University, SUNY College of Environmental Science and Forestry, West Virginia University, Delaware State University, Ohio State University, Rutgers University, USDA's Eastern Regional Research Center, and the U.S. Department of Energy's Oak Ridge National Laboratory and Idaho National Laboratory. Researchers involved in the project include plant scientists, agricultural and biological engineers, agricultural safety and health specialists, agronomists, agricultural and forest economists, rural sociologists, supply-chain and business-development experts, and extension educators. "The shrub willow stand at Rockview can continue producing biomass for more than 20 years, and we hope to use it both as a source of renewable energy and as a platform for sustainability research," said Armen Kemanian, associate professor of production systems and modeling in the Department of Plant Science, one of the lead researchers in the project. "This is an excellent site to investigate impacts on soil and water quality, biodiversity, avoided carbon dioxide emissions, and the potential for growing a regional bio-based economy," he said. "Students from our college visit the site and have a firsthand and close-up view of this new crop for the region." Why shrub willow? Because the woody perennial likes to be cut, explained Kemanian. He noted that visitors to Grand Teton National Park in Wyoming may remember the "willow flats," grazed to a uniform height by moose and elk. "At the Rockview site we don't have moose, but we do take advantage of shrub willow's vigorous regrowth to harvest for multiple cycles," he said. "As perennial plants, they establish a root system that stabilizes the soil and stores substantial amounts of carbon that otherwise would be lost to the atmosphere." Perennial biomass crops shrub willow, switchgrass and miscanthus—all of which are being investigated at other experimental sites around the Northeast—also store and recycle nutrients, so they do not require much fertilizer and can improve water quality in streams, rivers and estuaries, such as the Chesapeake Bay. Increasing perennial vegetation is a critical component of Pennsylvania's water quality strategy, and these biomass crops allow vulnerable parts of the landscape to remain economically productive while protecting water quality. Shrub willow can produce the same amount of biomass as a corn crop with only a third of the nitrogen fertilizer, Kemanian pointed out. When the plants grow, they take carbon dioxide from the atmosphere. After harvest, when the biomass is combusted either as wood chips or as a liquid biofuel, the carbon dioxide returns to the atmosphere to complete the cycle. Felipe Montes, a research associate in the Department of Plant Science, established an array of sensors to measure carbon dioxide and water vapor fluxes, which are giving a vivid picture of the growth potential in the region. Shrub willow is one the first plants to leaf out in early spring and dies back late in the fall, and this long growing season makes it extremely efficient in converting sunlight and nutrients to a bioenergy feedstock. "We estimate that we can harvest 20 to 30 units of energy per unit of fossil energy invested in producing the crop, leading to fuel with a very low carbon footprint," Montes said. "The fact that this biomass can be converted to liquid fuel is one of the main advantages of shrub willow and other biomass crops. Low carbon liquid fuels are especially important for long distance transportation, shipping and aviation, where electric vehicles are not practical." Biomass energy could provide the social, economic and ecological drivers for a sustainable rural renaissance in the Northeast, according to NEWBio project leader Tom Richard, professor of agricultural and biological engineering and director of the Penn State Institutes of Energy and the Environment. He believes perennial energy crops are particularly well suited for the region, where forests and pasture long have dominated the landscape. Rocky and sloped soils are more compatible with perennial crops, while perennial root systems better tolerate wet springs and occasional summer drought, Richard said. Northeast biomass production has high water-use efficiency (biomass produced per unit of water transpired by plants) owing to the region's moderate temperatures and relatively high humidity. These perennial crops also increase organic matter in the soil, and coupled with efficient refining and manufacturing processes can produce carbon-negative energy and materials. "Concerns about energy, environmental and human health, rural economic development, and the need to diversify agricultural products and markets have made the development of sustainably produced biomass feedstocks for biofuels, bioproducts and bioenergy a critical national priority," said Richard. "Perennial bioenergy systems, such as the shrub willow demonstrated at Penn State, appear to hold an important key to future economic development for our region. But to unlock that future, we need to learn how to economically handle the harvesting, transportation and storage of massive volumes, which constitutes 40 to 60 percent of the cost of biomass. This project is providing the knowledge and experience needed for a regional bioeconomy to achieve commercial success." Explore further: Bioenergy crops could store more carbon in soil

Renye Jr. J.A.,Eastern Regional Research Center | Somkuti G.A.,Eastern Regional Research Center
Biotechnology Letters | Year: 2012

The integrative vector, pINTRS, was used to transfer glutamate decarboxylase (GAD) activity to Streptococcus thermophilus ST128 thereby allowing for the production of γ-aminobutyric acid (GABA). In pINTRS, the gene encoding glutamate decarboxylase, gadB, was flanked by DNA fragments homologous to a S. thermophilus pseudogene to allow for integration at a non-essential locus on the chromosome. Screening techniques confirmed the insertion of gadB with either its endogenous promoter or the S. thermophilus P2201 promoter, resulting in the generation of recombinant strains, ST128/gadB or ST128/P2201-gadB. Following the integration event unwanted plasmid DNA, specifically the erythromycin resistance gene, was eliminated from the recombinant strains. Based on the production of GABA, activities of GAD for ST128/gadB and ST128/P2201-gadB were 30. 6 ± 6 and 27. 9 ± 7. 2 μM/mg dry cell wt, respectively. © 2011 Springer Science+Business Media B.V. (outside the USA).

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